Histone H1 and CDK1 Kinase Activities in Early Embryos of Four Mouse Strains after X-irradiation

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@article{2f9dcd84e58c4ae8a6b3803b52652500,
title = "Histone H1 and CDK1 Kinase Activities in Early Embryos of Four Mouse Strains after X-irradiation",
abstract = "The cdk1/cyclin B1 complex is a universal regulator known to be responsible for driving the cell cycle from the G2- to the M-phase. To investigate the effects of irradiation on the activity of this complex in preimplantation embryos, we irradiated one- and two-cell mouse embryos with X-rays, and measured the fluctuations of histone H1 and cdk1 kinase activity. Four mouse strains with different radiation sensitivities were chosen: the BALB/c and the Heiligenberger (radiation-sensitive) and the C57BL and the CF1 (radiation-resistant) strains. Embryos irradiated in the first cell cycle arrested in the G2-phase. However, the dynamics of this radiation-induced G2-block was different between the mouse strains tested. X-irradiation is known to induce a change in the phosphorylation state of the cdk1 protein kinase in adult somatic cells. In embryos from the BALB/c and C57BL strains, the histone H1 kinase activities were confirmed by the cdk1 phosphorylation pattern. X-irradiation at the 2-cell stage only induced a short G2-arrest in all tested mouse strains. In conclusion, cell cycle effects in early embryos under normal conditions and after irradiation are strictly paralleled by changes in the activity of the central cell cycle driving enzyme complex.",
keywords = "histone H1 kinase activity, cdk1, oocyte, embryo, irradiation, mouse, BALB/c, C57BL, Heiligenberger, CF1.",
author = "Sarah Baatout and Wolfgang M{\"u}ller and Arlette Michaux and Jasmine Buset and Werner Schoonjans and Paul Jacquet and Hanane Derradji and Myriam Ghardi and Khalil Abouelaradat",
note = "Score = 10",
year = "2007",
month = aug,
day = "20",
language = "English",
volume = "21",
pages = "571--582",
journal = "In Vivo",
issn = "0258-851X",
publisher = "IIAR - International Institute of Anticancer Research",
number = "4",

}

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TY - JOUR

T1 - Histone H1 and CDK1 Kinase Activities in Early Embryos of Four Mouse Strains after X-irradiation

AU - Baatout, Sarah

AU - Müller, Wolfgang

AU - Michaux, Arlette

AU - Buset, Jasmine

AU - Schoonjans, Werner

AU - Jacquet, Paul

A2 - Derradji, Hanane

A2 - Ghardi, Myriam

A2 - Abouelaradat, Khalil

N1 - Score = 10

PY - 2007/8/20

Y1 - 2007/8/20

N2 - The cdk1/cyclin B1 complex is a universal regulator known to be responsible for driving the cell cycle from the G2- to the M-phase. To investigate the effects of irradiation on the activity of this complex in preimplantation embryos, we irradiated one- and two-cell mouse embryos with X-rays, and measured the fluctuations of histone H1 and cdk1 kinase activity. Four mouse strains with different radiation sensitivities were chosen: the BALB/c and the Heiligenberger (radiation-sensitive) and the C57BL and the CF1 (radiation-resistant) strains. Embryos irradiated in the first cell cycle arrested in the G2-phase. However, the dynamics of this radiation-induced G2-block was different between the mouse strains tested. X-irradiation is known to induce a change in the phosphorylation state of the cdk1 protein kinase in adult somatic cells. In embryos from the BALB/c and C57BL strains, the histone H1 kinase activities were confirmed by the cdk1 phosphorylation pattern. X-irradiation at the 2-cell stage only induced a short G2-arrest in all tested mouse strains. In conclusion, cell cycle effects in early embryos under normal conditions and after irradiation are strictly paralleled by changes in the activity of the central cell cycle driving enzyme complex.

AB - The cdk1/cyclin B1 complex is a universal regulator known to be responsible for driving the cell cycle from the G2- to the M-phase. To investigate the effects of irradiation on the activity of this complex in preimplantation embryos, we irradiated one- and two-cell mouse embryos with X-rays, and measured the fluctuations of histone H1 and cdk1 kinase activity. Four mouse strains with different radiation sensitivities were chosen: the BALB/c and the Heiligenberger (radiation-sensitive) and the C57BL and the CF1 (radiation-resistant) strains. Embryos irradiated in the first cell cycle arrested in the G2-phase. However, the dynamics of this radiation-induced G2-block was different between the mouse strains tested. X-irradiation is known to induce a change in the phosphorylation state of the cdk1 protein kinase in adult somatic cells. In embryos from the BALB/c and C57BL strains, the histone H1 kinase activities were confirmed by the cdk1 phosphorylation pattern. X-irradiation at the 2-cell stage only induced a short G2-arrest in all tested mouse strains. In conclusion, cell cycle effects in early embryos under normal conditions and after irradiation are strictly paralleled by changes in the activity of the central cell cycle driving enzyme complex.

KW - histone H1 kinase activity

KW - cdk1

KW - oocyte

KW - embryo

KW - irradiation

KW - mouse

KW - BALB/c

KW - C57BL

KW - Heiligenberger

KW - CF1.

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_82290

UR - http://knowledgecentre.sckcen.be/so2/bibref/4378

M3 - Article

VL - 21

SP - 571

EP - 582

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 4

ER -

ID: 381484