In Utero Low Dose Irradiation Leads to Persistent Alterations in the Mouse Heart Proteome

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In Utero Low Dose Irradiation Leads to Persistent Alterations in the Mouse Heart Proteome. / Bakshi, Mayur V; Azimzadee"h, Omid; Merl-Pham, Juliane; Verreet, Tine; Hauck, Stefanie M; Benotmane, Rafi; Atkinson, Michael J; Tapio, Soile; Baatout, Sarah (Peer reviewer).

In: PLOS ONE, Vol. 11, No. 6, 08.06.2016.

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Bakshi, Mayur V ; Azimzadee"h, Omid ; Merl-Pham, Juliane ; Verreet, Tine ; Hauck, Stefanie M ; Benotmane, Rafi ; Atkinson, Michael J ; Tapio, Soile ; Baatout, Sarah. / In Utero Low Dose Irradiation Leads to Persistent Alterations in the Mouse Heart Proteome. In: PLOS ONE. 2016 ; Vol. 11, No. 6.

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@article{0b985c5888d54d0c8be4fe872a578fce,
title = "In Utero Low Dose Irradiation Leads to Persistent Alterations in the Mouse Heart Proteome",
abstract = "Prenatal exposure to stress such as increased level of reactive oxygen species or antiviraltherapy are known factors leading to adult heart defects. The risks following a radiationexposure during fetal period are unknown, as are the mechanisms of any potential cardiacdamage. The aim of this study was to gather evidence for possible damage by investigatinglong-term changes in the mouse heart proteome after prenatal exposure to low and moderateradiation doses. Pregnant C57Bl/6J mice received on embryonic day 11 (E11) a singletotal body dose of ionizing radiation that ranged from 0.02 Gy to 1.0 Gy. The offspring weresacrificed at the age of 6 months or 2 years. Quantitative proteomic analysis of heart tissuewas performed using Isotope Coded Protein Label technology and tandem mass spectrometry.The proteomics data were analyzed by bioinformatics and key changes were validatedby immunoblotting. Persistent changes were observed in the expression of proteins representingmitochondrial respiratory complexes, redox and heat shock response, and the cytoskeleton,even at the low dose of 0.1 Gy. The level of total and active form of the kinaseMAP4K4 that is essential for the embryonic development of mouse heart was",
keywords = "in utero, heart, proteome",
author = "Bakshi, {Mayur V} and Omid Azimzadee{"}h and Juliane Merl-Pham and Tine Verreet and Hauck, {Stefanie M} and Rafi Benotmane and Atkinson, {Michael J} and Soile Tapio and Sarah Baatout",
note = "Score=10",
year = "2016",
month = "6",
day = "8",
doi = "10.1371/journal.pone.0156952",
language = "English",
volume = "11",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "PLOS - Public Library of Science",
number = "6",

}

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TY - JOUR

T1 - In Utero Low Dose Irradiation Leads to Persistent Alterations in the Mouse Heart Proteome

AU - Bakshi, Mayur V

AU - Azimzadee"h, Omid

AU - Merl-Pham, Juliane

AU - Verreet, Tine

AU - Hauck, Stefanie M

AU - Benotmane, Rafi

AU - Atkinson, Michael J

AU - Tapio, Soile

A2 - Baatout, Sarah

N1 - Score=10

PY - 2016/6/8

Y1 - 2016/6/8

N2 - Prenatal exposure to stress such as increased level of reactive oxygen species or antiviraltherapy are known factors leading to adult heart defects. The risks following a radiationexposure during fetal period are unknown, as are the mechanisms of any potential cardiacdamage. The aim of this study was to gather evidence for possible damage by investigatinglong-term changes in the mouse heart proteome after prenatal exposure to low and moderateradiation doses. Pregnant C57Bl/6J mice received on embryonic day 11 (E11) a singletotal body dose of ionizing radiation that ranged from 0.02 Gy to 1.0 Gy. The offspring weresacrificed at the age of 6 months or 2 years. Quantitative proteomic analysis of heart tissuewas performed using Isotope Coded Protein Label technology and tandem mass spectrometry.The proteomics data were analyzed by bioinformatics and key changes were validatedby immunoblotting. Persistent changes were observed in the expression of proteins representingmitochondrial respiratory complexes, redox and heat shock response, and the cytoskeleton,even at the low dose of 0.1 Gy. The level of total and active form of the kinaseMAP4K4 that is essential for the embryonic development of mouse heart was

AB - Prenatal exposure to stress such as increased level of reactive oxygen species or antiviraltherapy are known factors leading to adult heart defects. The risks following a radiationexposure during fetal period are unknown, as are the mechanisms of any potential cardiacdamage. The aim of this study was to gather evidence for possible damage by investigatinglong-term changes in the mouse heart proteome after prenatal exposure to low and moderateradiation doses. Pregnant C57Bl/6J mice received on embryonic day 11 (E11) a singletotal body dose of ionizing radiation that ranged from 0.02 Gy to 1.0 Gy. The offspring weresacrificed at the age of 6 months or 2 years. Quantitative proteomic analysis of heart tissuewas performed using Isotope Coded Protein Label technology and tandem mass spectrometry.The proteomics data were analyzed by bioinformatics and key changes were validatedby immunoblotting. Persistent changes were observed in the expression of proteins representingmitochondrial respiratory complexes, redox and heat shock response, and the cytoskeleton,even at the low dose of 0.1 Gy. The level of total and active form of the kinaseMAP4K4 that is essential for the embryonic development of mouse heart was

KW - in utero

KW - heart

KW - proteome

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/19600859

U2 - 10.1371/journal.pone.0156952

DO - 10.1371/journal.pone.0156952

M3 - Article

VL - 11

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 6

ER -

ID: 1452074