Introduction to the principles and methods underlying the recovery of metagenome‐assembled genomes from metagenomic data

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  • UGent - Universiteit Gent

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Abstract

The rise of metagenomics offers a leap forward for understanding the genetic diversity of microorganisms in many different complex environments by providing a platform that can identify potentially unlimited numbers of known and novel microorganisms. As such, it is impossible to imagine new major initiatives without metagenomics. Nevertheless, it represents a relatively new discipline with various levels of complexity and demands on bioinformatics. The underlying principles and methods used in metagenomics are often seen as common knowledge and often not detailed or fragmented. Therefore, we reviewed these to guide microbiologists in taking the first steps into metagenomics. We specifically focus on a workflow aimed at reconstructing individual genomes, that is, metagenome‐assembled genomes, integrating DNA sequencing, assembly, binning, identification and annotation

Details

Original languageEnglish
Article numbere1298
Pages (from-to)1-19
Number of pages19
JournalMicrobiology Open
Volume11
Issue number3
DOIs
Publication statusPublished - 9 Jun 2022

Keywords

  • Annotation, Assembly, Binning, Metagenome‐assembled genome, Metagenomics, Sequencing

ID: 7723514