Research output: Contribution to journal › Article › peer-review
Introduction to the principles and methods underlying the recovery of metagenome‐assembled genomes from metagenomic data. / Goussarov, Gleb; Ahmed, Mohamed; Vandamme, Peter; Van Houdt, Rob.
In: Microbiology Open, Vol. 11, No. 3, e1298, 09.06.2022, p. 1-19.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Introduction to the principles and methods underlying the recovery of metagenome‐assembled genomes from metagenomic data
AU - Goussarov, Gleb
AU - Ahmed, Mohamed
AU - Vandamme, Peter
AU - Van Houdt, Rob
N1 - Score=10
PY - 2022/6/9
Y1 - 2022/6/9
N2 - The rise of metagenomics offers a leap forward for understanding the genetic diversity of microorganisms in many different complex environments by providing a platform that can identify potentially unlimited numbers of known and novel microorganisms. As such, it is impossible to imagine new major initiatives without metagenomics. Nevertheless, it represents a relatively new discipline with various levels of complexity and demands on bioinformatics. The underlying principles and methods used in metagenomics are often seen as common knowledge and often not detailed or fragmented. Therefore, we reviewed these to guide microbiologists in taking the first steps into metagenomics. We specifically focus on a workflow aimed at reconstructing individual genomes, that is, metagenome‐assembled genomes, integrating DNA sequencing, assembly, binning, identification and annotation
AB - The rise of metagenomics offers a leap forward for understanding the genetic diversity of microorganisms in many different complex environments by providing a platform that can identify potentially unlimited numbers of known and novel microorganisms. As such, it is impossible to imagine new major initiatives without metagenomics. Nevertheless, it represents a relatively new discipline with various levels of complexity and demands on bioinformatics. The underlying principles and methods used in metagenomics are often seen as common knowledge and often not detailed or fragmented. Therefore, we reviewed these to guide microbiologists in taking the first steps into metagenomics. We specifically focus on a workflow aimed at reconstructing individual genomes, that is, metagenome‐assembled genomes, integrating DNA sequencing, assembly, binning, identification and annotation
KW - Annotation
KW - Assembly
KW - Binning
KW - Metagenome‐assembled genome
KW - Metagenomics
KW - Sequencing
UR - https://ecm.sckcen.be/OTCS/llisapi.dll/open/49593257
U2 - 10.1002/mbo3.1298
DO - 10.1002/mbo3.1298
M3 - Article
VL - 11
SP - 1
EP - 19
JO - Microbiology Open
JF - Microbiology Open
SN - 2045-8827
IS - 3
M1 - e1298
ER -
ID: 7723514