Introduction to the principles and methods underlying the recovery of metagenome‐assembled genomes from metagenomic data

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Introduction to the principles and methods underlying the recovery of metagenome‐assembled genomes from metagenomic data. / Goussarov, Gleb; Ahmed, Mohamed; Vandamme, Peter; Van Houdt, Rob.

In: Microbiology Open, Vol. 11, No. 3, e1298, 09.06.2022, p. 1-19.

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@article{bc1cab22e962445daf04a948d464a458,
title = "Introduction to the principles and methods underlying the recovery of metagenome‐assembled genomes from metagenomic data",
abstract = "The rise of metagenomics offers a leap forward for understanding the genetic diversity of microorganisms in many different complex environments by providing a platform that can identify potentially unlimited numbers of known and novel microorganisms. As such, it is impossible to imagine new major initiatives without metagenomics. Nevertheless, it represents a relatively new discipline with various levels of complexity and demands on bioinformatics. The underlying principles and methods used in metagenomics are often seen as common knowledge and often not detailed or fragmented. Therefore, we reviewed these to guide microbiologists in taking the first steps into metagenomics. We specifically focus on a workflow aimed at reconstructing individual genomes, that is, metagenome‐assembled genomes, integrating DNA sequencing, assembly, binning, identification and annotation",
keywords = "Annotation, Assembly, Binning, Metagenome‐assembled genome, Metagenomics, Sequencing",
author = "Gleb Goussarov and Mohamed Ahmed and Peter Vandamme and {Van Houdt}, Rob",
note = "Score=10",
year = "2022",
month = jun,
day = "9",
doi = "10.1002/mbo3.1298",
language = "English",
volume = "11",
pages = "1--19",
journal = "Microbiology Open",
issn = "2045-8827",
publisher = "Wiley - John Wiley & Sons, Ltd",
number = "3",

}

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TY - JOUR

T1 - Introduction to the principles and methods underlying the recovery of metagenome‐assembled genomes from metagenomic data

AU - Goussarov, Gleb

AU - Ahmed, Mohamed

AU - Vandamme, Peter

AU - Van Houdt, Rob

N1 - Score=10

PY - 2022/6/9

Y1 - 2022/6/9

N2 - The rise of metagenomics offers a leap forward for understanding the genetic diversity of microorganisms in many different complex environments by providing a platform that can identify potentially unlimited numbers of known and novel microorganisms. As such, it is impossible to imagine new major initiatives without metagenomics. Nevertheless, it represents a relatively new discipline with various levels of complexity and demands on bioinformatics. The underlying principles and methods used in metagenomics are often seen as common knowledge and often not detailed or fragmented. Therefore, we reviewed these to guide microbiologists in taking the first steps into metagenomics. We specifically focus on a workflow aimed at reconstructing individual genomes, that is, metagenome‐assembled genomes, integrating DNA sequencing, assembly, binning, identification and annotation

AB - The rise of metagenomics offers a leap forward for understanding the genetic diversity of microorganisms in many different complex environments by providing a platform that can identify potentially unlimited numbers of known and novel microorganisms. As such, it is impossible to imagine new major initiatives without metagenomics. Nevertheless, it represents a relatively new discipline with various levels of complexity and demands on bioinformatics. The underlying principles and methods used in metagenomics are often seen as common knowledge and often not detailed or fragmented. Therefore, we reviewed these to guide microbiologists in taking the first steps into metagenomics. We specifically focus on a workflow aimed at reconstructing individual genomes, that is, metagenome‐assembled genomes, integrating DNA sequencing, assembly, binning, identification and annotation

KW - Annotation

KW - Assembly

KW - Binning

KW - Metagenome‐assembled genome

KW - Metagenomics

KW - Sequencing

UR - https://ecm.sckcen.be/OTCS/llisapi.dll/open/49593257

U2 - 10.1002/mbo3.1298

DO - 10.1002/mbo3.1298

M3 - Article

VL - 11

SP - 1

EP - 19

JO - Microbiology Open

JF - Microbiology Open

SN - 2045-8827

IS - 3

M1 - e1298

ER -

ID: 7723514