Iodine dose of administered contrast media affects the level of radiation-induced DNA damage during cardiac CT scans

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Iodine dose of administered contrast media affects the level of radiation-induced DNA damage during cardiac CT scans. / Van Cauteren, T. ; Honorio da Silva, Edilaine; Van Gompel, G.; Kersemans, V.; Sermon, K.; De Mey, J.; Buls, Nico.

In: American Journal of Roentgenology, Vol. 213, No. 2, 08.2019, p. 404-409.

Research output: Contribution to journalArticle

Harvard

Van Cauteren, T, Honorio da Silva, E, Van Gompel, G, Kersemans, V, Sermon, K, De Mey, J & Buls, N 2019, 'Iodine dose of administered contrast media affects the level of radiation-induced DNA damage during cardiac CT scans', American Journal of Roentgenology, vol. 213, no. 2, pp. 404-409. https://doi.org/10.2214/AJR.18.20950

APA

Van Cauteren, T., Honorio da Silva, E., Van Gompel, G., Kersemans, V., Sermon, K., De Mey, J., & Buls, N. (2019). Iodine dose of administered contrast media affects the level of radiation-induced DNA damage during cardiac CT scans. American Journal of Roentgenology, 213(2), 404-409. https://doi.org/10.2214/AJR.18.20950

Vancouver

Van Cauteren T, Honorio da Silva E, Van Gompel G, Kersemans V, Sermon K, De Mey J et al. Iodine dose of administered contrast media affects the level of radiation-induced DNA damage during cardiac CT scans. American Journal of Roentgenology. 2019 Aug;213(2):404-409. https://doi.org/10.2214/AJR.18.20950

Author

Van Cauteren, T. ; Honorio da Silva, Edilaine ; Van Gompel, G. ; Kersemans, V. ; Sermon, K. ; De Mey, J. ; Buls, Nico. / Iodine dose of administered contrast media affects the level of radiation-induced DNA damage during cardiac CT scans. In: American Journal of Roentgenology. 2019 ; Vol. 213, No. 2. pp. 404-409.

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@article{a780590b9d57440d817ea78cfb1845e6,
title = "Iodine dose of administered contrast media affects the level of radiation-induced DNA damage during cardiac CT scans",
abstract = "OBJECTIVE. The purpose of this study is to investigate the contributing effect of contrast media (CM) iodine dose on radiation-induced DNA damage in blood lymphocytes during a cardiac CT scan. MATERIALS AND METHODS. The minipigs were exposed 12 times in total to a fixed cardiac CT scan protocol. An unenhanced and two CM injection protocols were considered, the latter with 50{\%} saline diluted (160 mg I/mL) and standard iodixanol. Blood samples were collected before and after CT, and radiation-induced DNA double-strand breaks were assessed using γ-H2AX (H2A histone family member X) immunofluorescent staining of the blood lymphocytes. Significant differences in foci numbers were investigated with an independent sample t test. In addition, a numeric dosimetry model was applied that simulates the cardiac CT scan, with the heart represented by a blood volume containing a mixture of six iodine concentrations (0, 10, 20, 30, 40, and 50 mg I/mL). RESULTS. Compared with the unenhanced (0 mg I/mL) protocol, the number of γ-H2AX foci per cell increased significantly (p < 0.038), by 56.1{\%} for the reduced iodine dose (160 mg I/mL) and by 141.1{\%} for the standard iodine dose (320 mg I/mL) protocols. These in vivo results are confirmed by the dosimetry simulation model, in which 78.8{\%} and 133.7{\%} increases in locally absorbed blood dose in the left ventricle were observed for the reduced and standard iodine dose protocols, respectively. CONCLUSION. Administration of CM during a cardiac CT examination significantly increases radiation-induced DNA damage in blood lymphocytes. Moreover, a lower CM iodine dose results in a reduced level of DNA damage, at constant radiation exposure.",
keywords = "Cardiac CT, Iodinated contrast media, Minipigs, Patient safety, Radiation-induced DNA damage",
author = "{Van Cauteren}, T. and {Honorio da Silva}, Edilaine and {Van Gompel}, G. and V. Kersemans and K. Sermon and {De Mey}, J. and Nico Buls",
note = "Score=10",
year = "2019",
month = "8",
doi = "10.2214/AJR.18.20950",
language = "English",
volume = "213",
pages = "404--409",
journal = "American Journal of Roentgenology",
issn = "0361-803X",
publisher = "ARRS - American Roentgen Ray Society",
number = "2",

}

RIS - Download

TY - JOUR

T1 - Iodine dose of administered contrast media affects the level of radiation-induced DNA damage during cardiac CT scans

AU - Van Cauteren, T.

AU - Honorio da Silva, Edilaine

AU - Van Gompel, G.

AU - Kersemans, V.

AU - Sermon, K.

AU - De Mey, J.

AU - Buls, Nico

N1 - Score=10

PY - 2019/8

Y1 - 2019/8

N2 - OBJECTIVE. The purpose of this study is to investigate the contributing effect of contrast media (CM) iodine dose on radiation-induced DNA damage in blood lymphocytes during a cardiac CT scan. MATERIALS AND METHODS. The minipigs were exposed 12 times in total to a fixed cardiac CT scan protocol. An unenhanced and two CM injection protocols were considered, the latter with 50% saline diluted (160 mg I/mL) and standard iodixanol. Blood samples were collected before and after CT, and radiation-induced DNA double-strand breaks were assessed using γ-H2AX (H2A histone family member X) immunofluorescent staining of the blood lymphocytes. Significant differences in foci numbers were investigated with an independent sample t test. In addition, a numeric dosimetry model was applied that simulates the cardiac CT scan, with the heart represented by a blood volume containing a mixture of six iodine concentrations (0, 10, 20, 30, 40, and 50 mg I/mL). RESULTS. Compared with the unenhanced (0 mg I/mL) protocol, the number of γ-H2AX foci per cell increased significantly (p < 0.038), by 56.1% for the reduced iodine dose (160 mg I/mL) and by 141.1% for the standard iodine dose (320 mg I/mL) protocols. These in vivo results are confirmed by the dosimetry simulation model, in which 78.8% and 133.7% increases in locally absorbed blood dose in the left ventricle were observed for the reduced and standard iodine dose protocols, respectively. CONCLUSION. Administration of CM during a cardiac CT examination significantly increases radiation-induced DNA damage in blood lymphocytes. Moreover, a lower CM iodine dose results in a reduced level of DNA damage, at constant radiation exposure.

AB - OBJECTIVE. The purpose of this study is to investigate the contributing effect of contrast media (CM) iodine dose on radiation-induced DNA damage in blood lymphocytes during a cardiac CT scan. MATERIALS AND METHODS. The minipigs were exposed 12 times in total to a fixed cardiac CT scan protocol. An unenhanced and two CM injection protocols were considered, the latter with 50% saline diluted (160 mg I/mL) and standard iodixanol. Blood samples were collected before and after CT, and radiation-induced DNA double-strand breaks were assessed using γ-H2AX (H2A histone family member X) immunofluorescent staining of the blood lymphocytes. Significant differences in foci numbers were investigated with an independent sample t test. In addition, a numeric dosimetry model was applied that simulates the cardiac CT scan, with the heart represented by a blood volume containing a mixture of six iodine concentrations (0, 10, 20, 30, 40, and 50 mg I/mL). RESULTS. Compared with the unenhanced (0 mg I/mL) protocol, the number of γ-H2AX foci per cell increased significantly (p < 0.038), by 56.1% for the reduced iodine dose (160 mg I/mL) and by 141.1% for the standard iodine dose (320 mg I/mL) protocols. These in vivo results are confirmed by the dosimetry simulation model, in which 78.8% and 133.7% increases in locally absorbed blood dose in the left ventricle were observed for the reduced and standard iodine dose protocols, respectively. CONCLUSION. Administration of CM during a cardiac CT examination significantly increases radiation-induced DNA damage in blood lymphocytes. Moreover, a lower CM iodine dose results in a reduced level of DNA damage, at constant radiation exposure.

KW - Cardiac CT

KW - Iodinated contrast media

KW - Minipigs

KW - Patient safety

KW - Radiation-induced DNA damage

UR - https://ecm.sckcen.be/OTCS/llisapi.dll/overview/37611155

U2 - 10.2214/AJR.18.20950

DO - 10.2214/AJR.18.20950

M3 - Article

VL - 213

SP - 404

EP - 409

JO - American Journal of Roentgenology

JF - American Journal of Roentgenology

SN - 0361-803X

IS - 2

ER -

ID: 6743820