Large plasmids governing multiple resistances to heavy metals: a genetic approach

Research output: Contribution to journalArticle

Standard

Large plasmids governing multiple resistances to heavy metals: a genetic approach. / Diels, Ludovicus; Sadouk, A.; Mergeay, Max.

In: Toxicological and Environmental Chemistry, Vol. 23, No. 1-4, 1989, p. 79-89.

Research output: Contribution to journalArticle

Harvard

Author

Diels, Ludovicus ; Sadouk, A. ; Mergeay, Max. / Large plasmids governing multiple resistances to heavy metals: a genetic approach. In: Toxicological and Environmental Chemistry. 1989 ; Vol. 23, No. 1-4. pp. 79-89.

Bibtex - Download

@article{ca878c728957460193dd7cb60d924cce,
title = "Large plasmids governing multiple resistances to heavy metals: a genetic approach",
abstract = "Gram negative bacteria classified as Alcaligenes eutrophus and carrying large resistance plasmids (generally two) were found in various industrial sites highly contaminated by heavy metals (Zn++, Cu++, Co++,...). These strains were detected by DNA hybridization with a probe made with a 9kb fragment (ccz+ fragment) encoding for resistances to Cd++, Co++ and Zn++, and cloned from plasmid pMOL30. This plasmid was isolated from the representative strain A. eutrophus CH34 which harbours the plasmids pMOL30 (240 kb) and pMOL28 (165 kb). Phenotypes related to pMOL28 and pMOL30 include the tolerance to Cd++, Co++, Cr04 =, Cu++, Hg++, Ni++, Pb++ and Zn++. The described genetic properties of these plasmids refer to some cloned or mapped functions and to some plasmid rearrangements. Plasmid pMOL85 (250 kb) which is related to pMOL30 was also described. Its host (A. eutrophus DS185) was isolated from a zinc desert. pMOL85 can efficiently self transfer in plasmidfree derivatives.",
keywords = "Alcaligenes eutrophus, Heavy metals, Large plasmids, Resistance, Soil bacteria, Genotypes, Hypersensitivity, Nickel, Mercury, Zinc",
author = "Ludovicus Diels and A. Sadouk and Max Mergeay",
year = "1989",
doi = "10.1080/02772248909357453",
language = "English",
volume = "23",
pages = "79--89",
journal = "Toxicological and Environmental Chemistry",
issn = "0277-2248",
publisher = "Taylor & Francis (CRC)",
number = "1-4",

}

RIS - Download

TY - JOUR

T1 - Large plasmids governing multiple resistances to heavy metals: a genetic approach

AU - Diels, Ludovicus

AU - Sadouk, A.

AU - Mergeay, Max

PY - 1989

Y1 - 1989

N2 - Gram negative bacteria classified as Alcaligenes eutrophus and carrying large resistance plasmids (generally two) were found in various industrial sites highly contaminated by heavy metals (Zn++, Cu++, Co++,...). These strains were detected by DNA hybridization with a probe made with a 9kb fragment (ccz+ fragment) encoding for resistances to Cd++, Co++ and Zn++, and cloned from plasmid pMOL30. This plasmid was isolated from the representative strain A. eutrophus CH34 which harbours the plasmids pMOL30 (240 kb) and pMOL28 (165 kb). Phenotypes related to pMOL28 and pMOL30 include the tolerance to Cd++, Co++, Cr04 =, Cu++, Hg++, Ni++, Pb++ and Zn++. The described genetic properties of these plasmids refer to some cloned or mapped functions and to some plasmid rearrangements. Plasmid pMOL85 (250 kb) which is related to pMOL30 was also described. Its host (A. eutrophus DS185) was isolated from a zinc desert. pMOL85 can efficiently self transfer in plasmidfree derivatives.

AB - Gram negative bacteria classified as Alcaligenes eutrophus and carrying large resistance plasmids (generally two) were found in various industrial sites highly contaminated by heavy metals (Zn++, Cu++, Co++,...). These strains were detected by DNA hybridization with a probe made with a 9kb fragment (ccz+ fragment) encoding for resistances to Cd++, Co++ and Zn++, and cloned from plasmid pMOL30. This plasmid was isolated from the representative strain A. eutrophus CH34 which harbours the plasmids pMOL30 (240 kb) and pMOL28 (165 kb). Phenotypes related to pMOL28 and pMOL30 include the tolerance to Cd++, Co++, Cr04 =, Cu++, Hg++, Ni++, Pb++ and Zn++. The described genetic properties of these plasmids refer to some cloned or mapped functions and to some plasmid rearrangements. Plasmid pMOL85 (250 kb) which is related to pMOL30 was also described. Its host (A. eutrophus DS185) was isolated from a zinc desert. pMOL85 can efficiently self transfer in plasmidfree derivatives.

KW - Alcaligenes eutrophus

KW - Heavy metals

KW - Large plasmids

KW - Resistance

KW - Soil bacteria

KW - Genotypes

KW - Hypersensitivity

KW - Nickel

KW - Mercury

KW - Zinc

UR - https://ecm.sckcen.be/OTCS/llisapi.dll/overview/39652435

U2 - 10.1080/02772248909357453

DO - 10.1080/02772248909357453

M3 - Article

VL - 23

SP - 79

EP - 89

JO - Toxicological and Environmental Chemistry

JF - Toxicological and Environmental Chemistry

SN - 0277-2248

IS - 1-4

ER -

ID: 6901173