Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions

Research output: Contribution to journalArticlepeer-review

Standard

Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions. / Taghavi, Safiyh; Lesaulnier, Celine; Monchy, Sebastien; Wattiez, Ruddy; Mergeay, Max; van der Lelie, Daniel.

In: Antonie van Leeuwenhoek, Vol. 96, No. 2, 01.08.2009, p. 171-182.

Research output: Contribution to journalArticlepeer-review

Harvard

Taghavi, S, Lesaulnier, C, Monchy, S, Wattiez, R, Mergeay, M & van der Lelie, D 2009, 'Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions', Antonie van Leeuwenhoek, vol. 96, no. 2, pp. 171-182. https://doi.org/10.1007/s10482-008-9289-0

APA

Taghavi, S., Lesaulnier, C., Monchy, S., Wattiez, R., Mergeay, M., & van der Lelie, D. (2009). Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions. Antonie van Leeuwenhoek, 96(2), 171-182. https://doi.org/10.1007/s10482-008-9289-0

Vancouver

Taghavi S, Lesaulnier C, Monchy S, Wattiez R, Mergeay M, van der Lelie D. Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions. Antonie van Leeuwenhoek. 2009 Aug 1;96(2):171-182. https://doi.org/10.1007/s10482-008-9289-0

Author

Taghavi, Safiyh ; Lesaulnier, Celine ; Monchy, Sebastien ; Wattiez, Ruddy ; Mergeay, Max ; van der Lelie, Daniel. / Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions. In: Antonie van Leeuwenhoek. 2009 ; Vol. 96, No. 2. pp. 171-182.

Bibtex - Download

@article{d0bc7edac0b44281902293bc33ac7e6d,
title = "Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions",
abstract = "Proteome and transcriptome analysis, combined with mutagenesis, were used to better understand the response of Cupriavidus metallidurans CH34 against lead(II). Structural Pb(II)-resistance genes of the pMOL30-encoded pbrUTRABCD operon formed the major line of defense against Pb(II). Several general stress response mechanisms under the control of alternative sigma factors such as r24/rpoK, r32/rpoH and r28/fliA were induced. The expression of the pbrR2 cadA pbrC2 operon of the CMGI-1 region and the chromosomally encoded zntA were clearly induced in the presence of Pb(II), although their respective gene products were not detected via proteomics. After inactivation of the pbrA, pbrB or pbrD genes, the expression of the pbrR2 cadA pbrC2 operon went up considerably. This points towards synergistic interactions between pbrUTRABCD and pbrR2 cadA pbrC2 to maintain a low intracellular Pb(II) concentration, where pbrR2 cadA pbrC2 gene functions can complement and compensate for the mutations in the pbrA and pbrD genes. This role of zntA and cadA to complement for the loss of pbrA was further confirmed by mutation analysis. The pbrB::Tn(Km2) mutation resulted in the most significant decrease of Pb(II) resistance, indicating that Pb(II) sequestration, avoiding re-entry of this toxic metal ion, forms critical step in the pbr-encoded Pb(II) resistance mechanism.",
keywords = "Cupriavidus metallidurans CH34 - Lead (II) resistance pbrUTRABCD operon - zntA - pbrR2 cadA pbrC2 - Gene expression",
author = "Safiyh Taghavi and Celine Lesaulnier and Sebastien Monchy and Ruddy Wattiez and Max Mergeay and {van der Lelie}, Daniel",
note = "Score = 10",
year = "2009",
month = aug,
day = "1",
doi = "10.1007/s10482-008-9289-0",
language = "English",
volume = "96",
pages = "171--182",
journal = "Antonie van Leeuwenhoek",
issn = "0003-6072",
publisher = "Springer",
number = "2",

}

RIS - Download

TY - JOUR

T1 - Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions

AU - Taghavi, Safiyh

AU - Lesaulnier, Celine

AU - Monchy, Sebastien

AU - Wattiez, Ruddy

AU - Mergeay, Max

AU - van der Lelie, Daniel

N1 - Score = 10

PY - 2009/8/1

Y1 - 2009/8/1

N2 - Proteome and transcriptome analysis, combined with mutagenesis, were used to better understand the response of Cupriavidus metallidurans CH34 against lead(II). Structural Pb(II)-resistance genes of the pMOL30-encoded pbrUTRABCD operon formed the major line of defense against Pb(II). Several general stress response mechanisms under the control of alternative sigma factors such as r24/rpoK, r32/rpoH and r28/fliA were induced. The expression of the pbrR2 cadA pbrC2 operon of the CMGI-1 region and the chromosomally encoded zntA were clearly induced in the presence of Pb(II), although their respective gene products were not detected via proteomics. After inactivation of the pbrA, pbrB or pbrD genes, the expression of the pbrR2 cadA pbrC2 operon went up considerably. This points towards synergistic interactions between pbrUTRABCD and pbrR2 cadA pbrC2 to maintain a low intracellular Pb(II) concentration, where pbrR2 cadA pbrC2 gene functions can complement and compensate for the mutations in the pbrA and pbrD genes. This role of zntA and cadA to complement for the loss of pbrA was further confirmed by mutation analysis. The pbrB::Tn(Km2) mutation resulted in the most significant decrease of Pb(II) resistance, indicating that Pb(II) sequestration, avoiding re-entry of this toxic metal ion, forms critical step in the pbr-encoded Pb(II) resistance mechanism.

AB - Proteome and transcriptome analysis, combined with mutagenesis, were used to better understand the response of Cupriavidus metallidurans CH34 against lead(II). Structural Pb(II)-resistance genes of the pMOL30-encoded pbrUTRABCD operon formed the major line of defense against Pb(II). Several general stress response mechanisms under the control of alternative sigma factors such as r24/rpoK, r32/rpoH and r28/fliA were induced. The expression of the pbrR2 cadA pbrC2 operon of the CMGI-1 region and the chromosomally encoded zntA were clearly induced in the presence of Pb(II), although their respective gene products were not detected via proteomics. After inactivation of the pbrA, pbrB or pbrD genes, the expression of the pbrR2 cadA pbrC2 operon went up considerably. This points towards synergistic interactions between pbrUTRABCD and pbrR2 cadA pbrC2 to maintain a low intracellular Pb(II) concentration, where pbrR2 cadA pbrC2 gene functions can complement and compensate for the mutations in the pbrA and pbrD genes. This role of zntA and cadA to complement for the loss of pbrA was further confirmed by mutation analysis. The pbrB::Tn(Km2) mutation resulted in the most significant decrease of Pb(II) resistance, indicating that Pb(II) sequestration, avoiding re-entry of this toxic metal ion, forms critical step in the pbr-encoded Pb(II) resistance mechanism.

KW - Cupriavidus metallidurans CH34 - Lead (II) resistance pbrUTRABCD operon - zntA - pbrR2 cadA pbrC2 - Gene expression

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_102022

UR - http://knowledgecentre.sckcen.be/so2/bibref/6458

U2 - 10.1007/s10482-008-9289-0

DO - 10.1007/s10482-008-9289-0

M3 - Article

VL - 96

SP - 171

EP - 182

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

SN - 0003-6072

IS - 2

ER -

ID: 141407