Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells

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Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells. / Abouelaradat, Khalil; Monsieurs, Pieter; Anastasov, Natasa; Atkinson, Mike; Derradji, Hanane; De Meyer, Tim; Bekaert, Sofie; Van Criekinge, Wim; Baatout, Sarah.

In: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 731, No. 1-2, 03.2012, p. 27-40.

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Harvard

Abouelaradat, K, Monsieurs, P, Anastasov, N, Atkinson, M, Derradji, H, De Meyer, T, Bekaert, S, Van Criekinge, W & Baatout, S 2012, 'Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells', Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, vol. 731, no. 1-2, pp. 27-40. https://doi.org/10.1016/j.mrfmmm.2011.10.006

Vancouver

Abouelaradat K, Monsieurs P, Anastasov N, Atkinson M, Derradji H, De Meyer T et al. Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2012 Mar;731(1-2):27-40. https://doi.org/10.1016/j.mrfmmm.2011.10.006

Author

Abouelaradat, Khalil ; Monsieurs, Pieter ; Anastasov, Natasa ; Atkinson, Mike ; Derradji, Hanane ; De Meyer, Tim ; Bekaert, Sofie ; Van Criekinge, Wim ; Baatout, Sarah. / Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells. In: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2012 ; Vol. 731, No. 1-2. pp. 27-40.

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@article{d42ec48e87be443786b2ff3a2c45a837,
title = "Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells",
abstract = "The high doses of radiation received in the wake of the Chernobyl incident have been linked to the increased appearance of thyroid cancer in the children living in the vicinity of the site. However, the data gathered on the effect of low doses of radiation on the thyroid remain limited. We have examined the genome wide transcriptional response of a culture of TPC-1 human cell line of papillary thyroid carcinoma origin with a RET/PTC1 translocation to various doses (0.0625, 0.5, and 4 Gy) of X-rays and compared it to response of thyroids with a RET/PTC3 translocation and against wild-type mouse thyroids irradiated with the same doses using microarrays. We have found considerable overlap at a high dose of 4 Gy in both RET/PTC-positive systems but no common genes at 62.5 mGy. In addition, the response of RET/PTC-positive system at all doses was distinct from the response of wild-type thyroids with both systems signaling down different pathways. Analysis of the response of microRNAs in TPC-1 cells revealed a radiation-responsive signature of microRNAs in addition to dose-responsive microRNAs. Our results point to the fact that a low dose of X-rays seems to have a significant proliferative effect on normal thyroids.",
keywords = "radiation, thyroid, epigenetics, methylomics, Chernobyl",
author = "Khalil Abouelaradat and Pieter Monsieurs and Natasa Anastasov and Mike Atkinson and Hanane Derradji and {De Meyer}, Tim and Sofie Bekaert and {Van Criekinge}, Wim and Sarah Baatout",
note = "Score = 10",
year = "2012",
month = "3",
doi = "10.1016/j.mrfmmm.2011.10.006",
language = "English",
volume = "731",
pages = "27--40",
journal = "Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis",
issn = "0027-5107",
publisher = "Elsevier",
number = "1-2",

}

RIS - Download

TY - JOUR

T1 - Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells

AU - Abouelaradat, Khalil

AU - Monsieurs, Pieter

AU - Anastasov, Natasa

AU - Atkinson, Mike

AU - Derradji, Hanane

AU - De Meyer, Tim

AU - Bekaert, Sofie

AU - Van Criekinge, Wim

AU - Baatout, Sarah

N1 - Score = 10

PY - 2012/3

Y1 - 2012/3

N2 - The high doses of radiation received in the wake of the Chernobyl incident have been linked to the increased appearance of thyroid cancer in the children living in the vicinity of the site. However, the data gathered on the effect of low doses of radiation on the thyroid remain limited. We have examined the genome wide transcriptional response of a culture of TPC-1 human cell line of papillary thyroid carcinoma origin with a RET/PTC1 translocation to various doses (0.0625, 0.5, and 4 Gy) of X-rays and compared it to response of thyroids with a RET/PTC3 translocation and against wild-type mouse thyroids irradiated with the same doses using microarrays. We have found considerable overlap at a high dose of 4 Gy in both RET/PTC-positive systems but no common genes at 62.5 mGy. In addition, the response of RET/PTC-positive system at all doses was distinct from the response of wild-type thyroids with both systems signaling down different pathways. Analysis of the response of microRNAs in TPC-1 cells revealed a radiation-responsive signature of microRNAs in addition to dose-responsive microRNAs. Our results point to the fact that a low dose of X-rays seems to have a significant proliferative effect on normal thyroids.

AB - The high doses of radiation received in the wake of the Chernobyl incident have been linked to the increased appearance of thyroid cancer in the children living in the vicinity of the site. However, the data gathered on the effect of low doses of radiation on the thyroid remain limited. We have examined the genome wide transcriptional response of a culture of TPC-1 human cell line of papillary thyroid carcinoma origin with a RET/PTC1 translocation to various doses (0.0625, 0.5, and 4 Gy) of X-rays and compared it to response of thyroids with a RET/PTC3 translocation and against wild-type mouse thyroids irradiated with the same doses using microarrays. We have found considerable overlap at a high dose of 4 Gy in both RET/PTC-positive systems but no common genes at 62.5 mGy. In addition, the response of RET/PTC-positive system at all doses was distinct from the response of wild-type thyroids with both systems signaling down different pathways. Analysis of the response of microRNAs in TPC-1 cells revealed a radiation-responsive signature of microRNAs in addition to dose-responsive microRNAs. Our results point to the fact that a low dose of X-rays seems to have a significant proliferative effect on normal thyroids.

KW - radiation

KW - thyroid

KW - epigenetics

KW - methylomics

KW - Chernobyl

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_118605

UR - http://knowledgecentre.sckcen.be/so2/bibref/8888

U2 - 10.1016/j.mrfmmm.2011.10.006

DO - 10.1016/j.mrfmmm.2011.10.006

M3 - Article

VL - 731

SP - 27

EP - 40

JO - Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

SN - 0027-5107

IS - 1-2

ER -

ID: 242115