Low dose radiation response in endothelial cells as biological model for radiation-related cardiovascular risks

Research output: Contribution to report/book/conference proceedingsIn-proceedings paper

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Low dose radiation response in endothelial cells as biological model for radiation-related cardiovascular risks. / Rombouts, Charlotte; Aerts, An; Beck, Michaël; De Vos, Winnok; Vanmarcke, Hans; Van Oostveldt, Patrick; Benotmane, Rafi; Baatout, Sarah.

Annalen van de Belgische Vereniging voor Stralingsbescherming. Belgium, 2014. p. 289-298.

Research output: Contribution to report/book/conference proceedingsIn-proceedings paper

Harvard

Rombouts, C, Aerts, A, Beck, M, De Vos, W, Vanmarcke, H, Van Oostveldt, P, Benotmane, R & Baatout, S 2014, Low dose radiation response in endothelial cells as biological model for radiation-related cardiovascular risks. in Annalen van de Belgische Vereniging voor Stralingsbescherming. Belgium, pp. 289-298, International Symposium 50 Years BVS - ABR: Challenges for Radiological Protection for the next 50 Years, Brussels, Belgium, 2013-04-08.

APA

Rombouts, C., Aerts, A., Beck, M., De Vos, W., Vanmarcke, H., Van Oostveldt, P., ... Baatout, S. (2014). Low dose radiation response in endothelial cells as biological model for radiation-related cardiovascular risks. In Annalen van de Belgische Vereniging voor Stralingsbescherming (pp. 289-298). Belgium.

Vancouver

Rombouts C, Aerts A, Beck M, De Vos W, Vanmarcke H, Van Oostveldt P et al. Low dose radiation response in endothelial cells as biological model for radiation-related cardiovascular risks. In Annalen van de Belgische Vereniging voor Stralingsbescherming. Belgium. 2014. p. 289-298

Author

Bibtex - Download

@inproceedings{b5eedbc5b2014c348d0cb770a616d000,
title = "Low dose radiation response in endothelial cells as biological model for radiation-related cardiovascular risks",
abstract = "Below 0.5 Gy there is no clear dose-risk relationship for cardiovascular disease (CVD) risk. Biological understanding of radiation-related CVD is required for defining accurately the dose-risk relationship. The endothelium forms the inner layer of the cardiovascular system and its physiological actions maintain normal cardiovascular functioning. Endothelial cells are thus considered a critical target in radiation-related CVD. Therefore, we evaluated the low (<0.5 Gy) and high (2; 5 Gy) dose radiation response, with respect to DNA damage and repair, cell cycle progression and apoptosis, in primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line. We demonstrated for the first time that acute low doses of X-rays induce DNA damage and apoptosis in endothelial cells. More radiation-induced apoptosis occurred in EA.hy926 cells implying a higher radiosensitivity of EA.hy926 compared to HUVEC for this endpoint, which should be taken into account when using these cells as models for studying the endothelium radiation response. Furthermore, our results point to a non-linear dose-response relationship for DNA damage in endothelial cells. Overall, our findings indicating cellular effects below 0.5 Gy, may influence on the long term the current radio protection system that assumes a threshold dose for non-cancer effects",
keywords = "endothelial cells, low dose radiation, cardiovascular disease",
author = "Charlotte Rombouts and An Aerts and Micha{\"e}l Beck and {De Vos}, Winnok and Hans Vanmarcke and {Van Oostveldt}, Patrick and Rafi Benotmane and Sarah Baatout",
note = "Score = 2",
year = "2014",
month = "1",
day = "29",
language = "English",
pages = "289--298",
booktitle = "Annalen van de Belgische Vereniging voor Stralingsbescherming",

}

RIS - Download

TY - GEN

T1 - Low dose radiation response in endothelial cells as biological model for radiation-related cardiovascular risks

AU - Rombouts, Charlotte

AU - Aerts, An

AU - Beck, Michaël

AU - De Vos, Winnok

AU - Vanmarcke, Hans

AU - Van Oostveldt, Patrick

AU - Benotmane, Rafi

AU - Baatout, Sarah

N1 - Score = 2

PY - 2014/1/29

Y1 - 2014/1/29

N2 - Below 0.5 Gy there is no clear dose-risk relationship for cardiovascular disease (CVD) risk. Biological understanding of radiation-related CVD is required for defining accurately the dose-risk relationship. The endothelium forms the inner layer of the cardiovascular system and its physiological actions maintain normal cardiovascular functioning. Endothelial cells are thus considered a critical target in radiation-related CVD. Therefore, we evaluated the low (<0.5 Gy) and high (2; 5 Gy) dose radiation response, with respect to DNA damage and repair, cell cycle progression and apoptosis, in primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line. We demonstrated for the first time that acute low doses of X-rays induce DNA damage and apoptosis in endothelial cells. More radiation-induced apoptosis occurred in EA.hy926 cells implying a higher radiosensitivity of EA.hy926 compared to HUVEC for this endpoint, which should be taken into account when using these cells as models for studying the endothelium radiation response. Furthermore, our results point to a non-linear dose-response relationship for DNA damage in endothelial cells. Overall, our findings indicating cellular effects below 0.5 Gy, may influence on the long term the current radio protection system that assumes a threshold dose for non-cancer effects

AB - Below 0.5 Gy there is no clear dose-risk relationship for cardiovascular disease (CVD) risk. Biological understanding of radiation-related CVD is required for defining accurately the dose-risk relationship. The endothelium forms the inner layer of the cardiovascular system and its physiological actions maintain normal cardiovascular functioning. Endothelial cells are thus considered a critical target in radiation-related CVD. Therefore, we evaluated the low (<0.5 Gy) and high (2; 5 Gy) dose radiation response, with respect to DNA damage and repair, cell cycle progression and apoptosis, in primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line. We demonstrated for the first time that acute low doses of X-rays induce DNA damage and apoptosis in endothelial cells. More radiation-induced apoptosis occurred in EA.hy926 cells implying a higher radiosensitivity of EA.hy926 compared to HUVEC for this endpoint, which should be taken into account when using these cells as models for studying the endothelium radiation response. Furthermore, our results point to a non-linear dose-response relationship for DNA damage in endothelial cells. Overall, our findings indicating cellular effects below 0.5 Gy, may influence on the long term the current radio protection system that assumes a threshold dose for non-cancer effects

KW - endothelial cells

KW - low dose radiation

KW - cardiovascular disease

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_133731

UR - http://knowledgecentre.sckcen.be/so2/bibref/11166

M3 - In-proceedings paper

SP - 289

EP - 298

BT - Annalen van de Belgische Vereniging voor Stralingsbescherming

CY - Belgium

ER -

ID: 95006