Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes

Research output: Contribution to journalArticlepeer-review

Standard

Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes. / El Saghire, Houssein; Michaux, Arlette; Thierens, Hubert; Baatout, Sarah.

In: International Journal of Molecular Medicine, Vol. 32, No. 6, 10.2013, p. 1407-1414.

Research output: Contribution to journalArticlepeer-review

Author

El Saghire, Houssein ; Michaux, Arlette ; Thierens, Hubert ; Baatout, Sarah. / Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes. In: International Journal of Molecular Medicine. 2013 ; Vol. 32, No. 6. pp. 1407-1414.

Bibtex - Download

@article{c526249e230c4e5197f12c3fb90c9bf3,
title = "Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes",
abstract = "Monocytes are key players in the induction of an immune response. Within the context of this study, we investigated the activa¬tion of toll-like receptors, mitogen activated protein kinases and NF-κB signaling in isolated human primary monocytes in response to low doses (0.05 and 0.1 Gy) and a high dose (1 Gy) of ionizing radiation. Using quantita¬tive RT-PCR and ELISA techniques, our results showed a positive regulation of TLR signaling in response to low doses but a less significant response at high doses. This activa¬tion was demonstrated via the activation of TLR signaling molecules. Furthermore, and in contrast to the high dose, the low doses showed increased phosphorylation levels of the protein IκBα, and therefore positive signaling of the NF-κB pathway. This result denotes pro-survival and pro-inflammatory responses. Additionally, MAPKs were activated in response to 0.05 Gy, while 0.1 and 1 Gy showed a downregulatory trend that may be related to activation of the PF4 gene. On the other hand, there was highly significant involvement of activated p53 and damaged genes in response to high but not low doses.",
keywords = "low dose, ionizing radiation, human primary monocytes, inflammatory response, NF-κB, MAPK, TLR, high dose, damage",
author = "{El Saghire}, Houssein and Arlette Michaux and Hubert Thierens and Sarah Baatout",
note = "Score = 10",
year = "2013",
month = oct,
doi = "10.3892/ijmm.2013.1514",
language = "English",
volume = "32",
pages = "1407--1414",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "6",

}

RIS - Download

TY - JOUR

T1 - Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes

AU - El Saghire, Houssein

AU - Michaux, Arlette

AU - Thierens, Hubert

AU - Baatout, Sarah

N1 - Score = 10

PY - 2013/10

Y1 - 2013/10

N2 - Monocytes are key players in the induction of an immune response. Within the context of this study, we investigated the activa¬tion of toll-like receptors, mitogen activated protein kinases and NF-κB signaling in isolated human primary monocytes in response to low doses (0.05 and 0.1 Gy) and a high dose (1 Gy) of ionizing radiation. Using quantita¬tive RT-PCR and ELISA techniques, our results showed a positive regulation of TLR signaling in response to low doses but a less significant response at high doses. This activa¬tion was demonstrated via the activation of TLR signaling molecules. Furthermore, and in contrast to the high dose, the low doses showed increased phosphorylation levels of the protein IκBα, and therefore positive signaling of the NF-κB pathway. This result denotes pro-survival and pro-inflammatory responses. Additionally, MAPKs were activated in response to 0.05 Gy, while 0.1 and 1 Gy showed a downregulatory trend that may be related to activation of the PF4 gene. On the other hand, there was highly significant involvement of activated p53 and damaged genes in response to high but not low doses.

AB - Monocytes are key players in the induction of an immune response. Within the context of this study, we investigated the activa¬tion of toll-like receptors, mitogen activated protein kinases and NF-κB signaling in isolated human primary monocytes in response to low doses (0.05 and 0.1 Gy) and a high dose (1 Gy) of ionizing radiation. Using quantita¬tive RT-PCR and ELISA techniques, our results showed a positive regulation of TLR signaling in response to low doses but a less significant response at high doses. This activa¬tion was demonstrated via the activation of TLR signaling molecules. Furthermore, and in contrast to the high dose, the low doses showed increased phosphorylation levels of the protein IκBα, and therefore positive signaling of the NF-κB pathway. This result denotes pro-survival and pro-inflammatory responses. Additionally, MAPKs were activated in response to 0.05 Gy, while 0.1 and 1 Gy showed a downregulatory trend that may be related to activation of the PF4 gene. On the other hand, there was highly significant involvement of activated p53 and damaged genes in response to high but not low doses.

KW - low dose

KW - ionizing radiation

KW - human primary monocytes

KW - inflammatory response

KW - NF-κB

KW - MAPK

KW - TLR

KW - high dose

KW - damage

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_131736

UR - http://knowledgecentre.sckcen.be/so2/bibref/10682

U2 - 10.3892/ijmm.2013.1514

DO - 10.3892/ijmm.2013.1514

M3 - Article

VL - 32

SP - 1407

EP - 1414

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 6

ER -

ID: 134580