Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth

Research output: Contribution to journalArticlepeer-review

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Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth. / Piroska, Virag; Hedisu, Mihaela; Soritau, Olga; Perde-Schrepler, Mari; Brie, Ioana; Pall, Emoke; Fischer-Fodor, Eva; Bogdan, Loredana; Lucaciu, Ondine; Belmans, Niels; Moreels, Marjan; Salmon, Benjamin; Jacobs, Reinhilde.

In: Dentomaxillofacial Radiology, Vol. 47, 20170462, 02.07.2018.

Research output: Contribution to journalArticlepeer-review

Harvard

Piroska, V, Hedisu, M, Soritau, O, Perde-Schrepler, M, Brie, I, Pall, E, Fischer-Fodor, E, Bogdan, L, Lucaciu, O, Belmans, N, Moreels, M, Salmon, B & Jacobs, R 2018, 'Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth', Dentomaxillofacial Radiology, vol. 47, 20170462. https://doi.org/10.1259/dmfr.20170462

APA

Piroska, V., Hedisu, M., Soritau, O., Perde-Schrepler, M., Brie, I., Pall, E., Fischer-Fodor, E., Bogdan, L., Lucaciu, O., Belmans, N., Moreels, M., Salmon, B., & Jacobs, R. (2018). Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth. Dentomaxillofacial Radiology, 47, [20170462]. https://doi.org/10.1259/dmfr.20170462

Vancouver

Piroska V, Hedisu M, Soritau O, Perde-Schrepler M, Brie I, Pall E et al. Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth. Dentomaxillofacial Radiology. 2018 Jul 2;47. 20170462. https://doi.org/10.1259/dmfr.20170462

Author

Piroska, Virag ; Hedisu, Mihaela ; Soritau, Olga ; Perde-Schrepler, Mari ; Brie, Ioana ; Pall, Emoke ; Fischer-Fodor, Eva ; Bogdan, Loredana ; Lucaciu, Ondine ; Belmans, Niels ; Moreels, Marjan ; Salmon, Benjamin ; Jacobs, Reinhilde. / Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth. In: Dentomaxillofacial Radiology. 2018 ; Vol. 47.

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@article{479076fe03e042aa8a755258216bbc72,
title = "Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth",
abstract = "Objectives: Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practice, was introduced also in pediatric radiology, especially orthodontics. Such patients subjected to repetitive X-rays examinations may receive substantial levels of radiation doses. Ionizing radiation (IR), a recognized carcinogenic factor causing DNA double-strand breaks (DSBs) could be harmful to undifferentiated cells such as dental pulp stem cells (DPSCs) since inaccurately repaired or unrepaired DSBs may lead to malignant transformation. The H2AX and MRE11 proteins generated following DSBs formation and pro-inflammatory cytokines (CKs) secreted after irradiation are relevant candidates to monitor the cellular responses induced by CBCT. Methods: DPSCs were extracted from human exfoliated deciduous teeth and their phenotype was assessed by immunocytochemistry and flow-cytometry. Cells were exposed to IR doses: 5.4–107.7 mGy, corresponding to 0.5–8 consecutive skull exposures, respectively. H2AX and MRE11 were detected in whole cells, while IL-1α, IL-6, IL-8, TNFα in supernatants, using enzyme-linked immunosorbent assay (ELISA) at different time points after exposure. Results: The phosphorylation level of H2AX in DPSCs increased considerably at 0.5 h after exposure (p <0.001 for 3, 5, 8 skull exposures and p <0.05 for 1 skull exposure, respectively). MRE11 response could only be detected for the highest IR dose (p <0.001) in the same interval. CKs secretion increased upon CBCT exposure according to doses and time. Conclusions: The DPSCs exposure to CBCT induces transient DNA damage and persistent inflammatory reaction in DPSCs drawing the attention on the potential risks of IR exposures and on the importance of dose monitoring in pediatric population.",
keywords = "Cone beam CT, DNA damage, dental stem cells",
author = "Virag Piroska and Mihaela Hedisu and Olga Soritau and Mari Perde-Schrepler and Ioana Brie and Emoke Pall and Eva Fischer-Fodor and Loredana Bogdan and Ondine Lucaciu and Niels Belmans and Marjan Moreels and Benjamin Salmon and Reinhilde Jacobs",
note = "Score=10",
year = "2018",
month = jul,
day = "2",
doi = "10.1259/dmfr.20170462",
language = "English",
volume = "47",
journal = "Dentomaxillofacial Radiology",
issn = "0250-832X",
publisher = "British Institute of Radiology",

}

RIS - Download

TY - JOUR

T1 - Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth

AU - Piroska, Virag

AU - Hedisu, Mihaela

AU - Soritau, Olga

AU - Perde-Schrepler, Mari

AU - Brie, Ioana

AU - Pall, Emoke

AU - Fischer-Fodor, Eva

AU - Bogdan, Loredana

AU - Lucaciu, Ondine

AU - Belmans, Niels

AU - Moreels, Marjan

AU - Salmon, Benjamin

AU - Jacobs, Reinhilde

N1 - Score=10

PY - 2018/7/2

Y1 - 2018/7/2

N2 - Objectives: Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practice, was introduced also in pediatric radiology, especially orthodontics. Such patients subjected to repetitive X-rays examinations may receive substantial levels of radiation doses. Ionizing radiation (IR), a recognized carcinogenic factor causing DNA double-strand breaks (DSBs) could be harmful to undifferentiated cells such as dental pulp stem cells (DPSCs) since inaccurately repaired or unrepaired DSBs may lead to malignant transformation. The H2AX and MRE11 proteins generated following DSBs formation and pro-inflammatory cytokines (CKs) secreted after irradiation are relevant candidates to monitor the cellular responses induced by CBCT. Methods: DPSCs were extracted from human exfoliated deciduous teeth and their phenotype was assessed by immunocytochemistry and flow-cytometry. Cells were exposed to IR doses: 5.4–107.7 mGy, corresponding to 0.5–8 consecutive skull exposures, respectively. H2AX and MRE11 were detected in whole cells, while IL-1α, IL-6, IL-8, TNFα in supernatants, using enzyme-linked immunosorbent assay (ELISA) at different time points after exposure. Results: The phosphorylation level of H2AX in DPSCs increased considerably at 0.5 h after exposure (p <0.001 for 3, 5, 8 skull exposures and p <0.05 for 1 skull exposure, respectively). MRE11 response could only be detected for the highest IR dose (p <0.001) in the same interval. CKs secretion increased upon CBCT exposure according to doses and time. Conclusions: The DPSCs exposure to CBCT induces transient DNA damage and persistent inflammatory reaction in DPSCs drawing the attention on the potential risks of IR exposures and on the importance of dose monitoring in pediatric population.

AB - Objectives: Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practice, was introduced also in pediatric radiology, especially orthodontics. Such patients subjected to repetitive X-rays examinations may receive substantial levels of radiation doses. Ionizing radiation (IR), a recognized carcinogenic factor causing DNA double-strand breaks (DSBs) could be harmful to undifferentiated cells such as dental pulp stem cells (DPSCs) since inaccurately repaired or unrepaired DSBs may lead to malignant transformation. The H2AX and MRE11 proteins generated following DSBs formation and pro-inflammatory cytokines (CKs) secreted after irradiation are relevant candidates to monitor the cellular responses induced by CBCT. Methods: DPSCs were extracted from human exfoliated deciduous teeth and their phenotype was assessed by immunocytochemistry and flow-cytometry. Cells were exposed to IR doses: 5.4–107.7 mGy, corresponding to 0.5–8 consecutive skull exposures, respectively. H2AX and MRE11 were detected in whole cells, while IL-1α, IL-6, IL-8, TNFα in supernatants, using enzyme-linked immunosorbent assay (ELISA) at different time points after exposure. Results: The phosphorylation level of H2AX in DPSCs increased considerably at 0.5 h after exposure (p <0.001 for 3, 5, 8 skull exposures and p <0.05 for 1 skull exposure, respectively). MRE11 response could only be detected for the highest IR dose (p <0.001) in the same interval. CKs secretion increased upon CBCT exposure according to doses and time. Conclusions: The DPSCs exposure to CBCT induces transient DNA damage and persistent inflammatory reaction in DPSCs drawing the attention on the potential risks of IR exposures and on the importance of dose monitoring in pediatric population.

KW - Cone beam CT

KW - DNA damage

KW - dental stem cells

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/31022880

U2 - 10.1259/dmfr.20170462

DO - 10.1259/dmfr.20170462

M3 - Article

VL - 47

JO - Dentomaxillofacial Radiology

JF - Dentomaxillofacial Radiology

SN - 0250-832X

M1 - 20170462

ER -

ID: 4521084