Medium-mediated DNA repair response after ionizing radiation is correlated with the increase of specific cytokines in human fibroblasts

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Medium-mediated DNA repair response after ionizing radiation is correlated with the increase of specific cytokines in human fibroblasts. / Dieriks, Birger; De Vos, Winnok; Derradji, Hanane; Baatout, Sarah; Van Oostveldt, Patrick; de Saint-Georges, Louis (Peer reviewer).

In: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 687, No. 1-2, 05.2010, p. 40-48.

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Dieriks, B, De Vos, W, Derradji, H, Baatout, S, Van Oostveldt, P & de Saint-Georges, L 2010, 'Medium-mediated DNA repair response after ionizing radiation is correlated with the increase of specific cytokines in human fibroblasts', Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, vol. 687, no. 1-2, pp. 40-48. https://doi.org/10.1016/J.mrfmmm.2010.01.011

Author

Dieriks, Birger ; De Vos, Winnok ; Derradji, Hanane ; Baatout, Sarah ; Van Oostveldt, Patrick ; de Saint-Georges, Louis. / Medium-mediated DNA repair response after ionizing radiation is correlated with the increase of specific cytokines in human fibroblasts. In: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2010 ; Vol. 687, No. 1-2. pp. 40-48.

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@article{3513027b59494af88dba5bfad2933319,
title = "Medium-mediated DNA repair response after ionizing radiation is correlated with the increase of specific cytokines in human fibroblasts",
abstract = "Radiation induced bystander effects, either protective or adverse, have been identified in a variety of cells and for different endpoints. They are thought to arise from communication between cells through direct cell–cell contacts and via transmissible molecules secreted into the medium by targeted cells. We have investigated medium-mediated damage response in human dermal fibroblasts (HDF) after exposure to ionizing irradiation. We show that HDF experience an dose-dependent elevated level of double stranded DNA damage repair response when incubated with conditioned growth medium of irradiated cells.Since secretion of cytokines is one of the possible pathways linking targeted and non-targeted cells a multiplex analysis was performed. Four cytokines – IL6, IL8, MCP-1 and RANTES – were identified in the growth medium of irradiated cells after exposure to X-rays (2 Gy). These cytokines were significantly upregulated and each cytokine showed differential upregulation kinetics. Finally we performed a functional analysis to see if IL6 and MCP-1 could induce γ-H2AX foci formation. IL6 caused a significant increase in spot occupancy compared to controls. The combined addition of IL-6 and MCP-1 produced no significant response. Both IL6 and MCP-1 have an effect on the γ-H2AX spot occupancy possibly linking these cytokines to the bystander response.",
keywords = "Fibroblasts, cytokines, DNA damage, Bystander effect, gamma-H2AX",
author = "Birger Dieriks and {De Vos}, Winnok and Hanane Derradji and Sarah Baatout and {Van Oostveldt}, Patrick and {de Saint-Georges}, Louis",
note = "Score = 10",
year = "2010",
month = may,
doi = "10.1016/J.mrfmmm.2010.01.011",
language = "English",
volume = "687",
pages = "40--48",
journal = "Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis",
issn = "0027-5107",
publisher = "Elsevier",
number = "1-2",

}

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TY - JOUR

T1 - Medium-mediated DNA repair response after ionizing radiation is correlated with the increase of specific cytokines in human fibroblasts

AU - Dieriks, Birger

AU - De Vos, Winnok

AU - Derradji, Hanane

AU - Baatout, Sarah

AU - Van Oostveldt, Patrick

A2 - de Saint-Georges, Louis

N1 - Score = 10

PY - 2010/5

Y1 - 2010/5

N2 - Radiation induced bystander effects, either protective or adverse, have been identified in a variety of cells and for different endpoints. They are thought to arise from communication between cells through direct cell–cell contacts and via transmissible molecules secreted into the medium by targeted cells. We have investigated medium-mediated damage response in human dermal fibroblasts (HDF) after exposure to ionizing irradiation. We show that HDF experience an dose-dependent elevated level of double stranded DNA damage repair response when incubated with conditioned growth medium of irradiated cells.Since secretion of cytokines is one of the possible pathways linking targeted and non-targeted cells a multiplex analysis was performed. Four cytokines – IL6, IL8, MCP-1 and RANTES – were identified in the growth medium of irradiated cells after exposure to X-rays (2 Gy). These cytokines were significantly upregulated and each cytokine showed differential upregulation kinetics. Finally we performed a functional analysis to see if IL6 and MCP-1 could induce γ-H2AX foci formation. IL6 caused a significant increase in spot occupancy compared to controls. The combined addition of IL-6 and MCP-1 produced no significant response. Both IL6 and MCP-1 have an effect on the γ-H2AX spot occupancy possibly linking these cytokines to the bystander response.

AB - Radiation induced bystander effects, either protective or adverse, have been identified in a variety of cells and for different endpoints. They are thought to arise from communication between cells through direct cell–cell contacts and via transmissible molecules secreted into the medium by targeted cells. We have investigated medium-mediated damage response in human dermal fibroblasts (HDF) after exposure to ionizing irradiation. We show that HDF experience an dose-dependent elevated level of double stranded DNA damage repair response when incubated with conditioned growth medium of irradiated cells.Since secretion of cytokines is one of the possible pathways linking targeted and non-targeted cells a multiplex analysis was performed. Four cytokines – IL6, IL8, MCP-1 and RANTES – were identified in the growth medium of irradiated cells after exposure to X-rays (2 Gy). These cytokines were significantly upregulated and each cytokine showed differential upregulation kinetics. Finally we performed a functional analysis to see if IL6 and MCP-1 could induce γ-H2AX foci formation. IL6 caused a significant increase in spot occupancy compared to controls. The combined addition of IL-6 and MCP-1 produced no significant response. Both IL6 and MCP-1 have an effect on the γ-H2AX spot occupancy possibly linking these cytokines to the bystander response.

KW - Fibroblasts

KW - cytokines

KW - DNA damage

KW - Bystander effect

KW - gamma-H2AX

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_105889

UR - http://knowledgecentre.sckcen.be/so2/bibref/6958

U2 - 10.1016/J.mrfmmm.2010.01.011

DO - 10.1016/J.mrfmmm.2010.01.011

M3 - Article

VL - 687

SP - 40

EP - 48

JO - Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

SN - 0027-5107

IS - 1-2

ER -

ID: 154127