Metal transport ATPase genes from Cupriavidus metallidurans CH34: a transcriptomic approach

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Metal transport ATPase genes from Cupriavidus metallidurans CH34: a transcriptomic approach. / Monchy, Sébastien; Vallaeys, Tatiana; Bossus, Albert; Mergeay, Max.

In: International Journal of Environmental Analytical Chemistry, Vol. 86, No. 9, 10.08.2006, p. 677-692.

Research output: Contribution to journalArticlepeer-review

Harvard

Monchy, S, Vallaeys, T, Bossus, A & Mergeay, M 2006, 'Metal transport ATPase genes from Cupriavidus metallidurans CH34: a transcriptomic approach', International Journal of Environmental Analytical Chemistry, vol. 86, no. 9, pp. 677-692. https://doi.org/10.1080/03067310600583824

APA

Monchy, S., Vallaeys, T., Bossus, A., & Mergeay, M. (2006). Metal transport ATPase genes from Cupriavidus metallidurans CH34: a transcriptomic approach. International Journal of Environmental Analytical Chemistry, 86(9), 677-692. https://doi.org/10.1080/03067310600583824

Vancouver

Monchy S, Vallaeys T, Bossus A, Mergeay M. Metal transport ATPase genes from Cupriavidus metallidurans CH34: a transcriptomic approach. International Journal of Environmental Analytical Chemistry. 2006 Aug 10;86(9):677-692. https://doi.org/10.1080/03067310600583824

Author

Monchy, Sébastien ; Vallaeys, Tatiana ; Bossus, Albert ; Mergeay, Max. / Metal transport ATPase genes from Cupriavidus metallidurans CH34: a transcriptomic approach. In: International Journal of Environmental Analytical Chemistry. 2006 ; Vol. 86, No. 9. pp. 677-692.

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@article{aea95b60c21e45fc8aa7f54270dfd163,
title = "Metal transport ATPase genes from Cupriavidus metallidurans CH34: a transcriptomic approach",
abstract = "Cupriavidus metallidurans strain CH34 is a multiple metal resistant -proteobacterium isolated from the sediments of a zinc metallurgical plant. This strain possesses a large diversity of heavy metal resistance genes that are located mostly on two large plasmids, pMOL28 and pMOL30. The metal efflux ATPases constitute the major mechanism by which metal ions are removed and pumped out from the cytoplasmic pool of thiol groups. The genome of C. metallidurans CH34 contains eight such P1-ATPases. This high number of ATPases, compared to the P1-ATPase content in other microorganisms, is a typical feature of the adaptation of C. metallidurans CH34 to metal-rich biotopes. We performed phylogenetic analysis, and used quantitative PCR to study the metal specific induction of the ATPases genes and, for some of them, their neighbouring genes. One of the ATPases, a homologue of the E. coli zntA gene, was characterized in detail, by analyzing the phenotypes of zntA mutants in different genetic backgrounds. In addition to the already reported sensitivity to Zn(II), Cd(II) and Pb(II), zntA mutants also displayed a higher sensitivity to Tl(I) and Bi(III), indicating that the ZntA protein might play role in the tolerance of very large cations.",
keywords = "Cupriavidus metallidurans, ralstonia, P-type ATPase, heavy metal, resistance, plasmids, quantitative PCR",
author = "S{\'e}bastien Monchy and Tatiana Vallaeys and Albert Bossus and Max Mergeay",
note = "Score = 10",
year = "2006",
month = aug,
day = "10",
doi = "10.1080/03067310600583824",
language = "English",
volume = "86",
pages = "677--692",
journal = "International Journal of Environmental Analytical Chemistry",
issn = "1029-0397",
publisher = "Taylor & Francis (CRC)",
number = "9",

}

RIS - Download

TY - JOUR

T1 - Metal transport ATPase genes from Cupriavidus metallidurans CH34: a transcriptomic approach

AU - Monchy, Sébastien

AU - Vallaeys, Tatiana

AU - Bossus, Albert

AU - Mergeay, Max

N1 - Score = 10

PY - 2006/8/10

Y1 - 2006/8/10

N2 - Cupriavidus metallidurans strain CH34 is a multiple metal resistant -proteobacterium isolated from the sediments of a zinc metallurgical plant. This strain possesses a large diversity of heavy metal resistance genes that are located mostly on two large plasmids, pMOL28 and pMOL30. The metal efflux ATPases constitute the major mechanism by which metal ions are removed and pumped out from the cytoplasmic pool of thiol groups. The genome of C. metallidurans CH34 contains eight such P1-ATPases. This high number of ATPases, compared to the P1-ATPase content in other microorganisms, is a typical feature of the adaptation of C. metallidurans CH34 to metal-rich biotopes. We performed phylogenetic analysis, and used quantitative PCR to study the metal specific induction of the ATPases genes and, for some of them, their neighbouring genes. One of the ATPases, a homologue of the E. coli zntA gene, was characterized in detail, by analyzing the phenotypes of zntA mutants in different genetic backgrounds. In addition to the already reported sensitivity to Zn(II), Cd(II) and Pb(II), zntA mutants also displayed a higher sensitivity to Tl(I) and Bi(III), indicating that the ZntA protein might play role in the tolerance of very large cations.

AB - Cupriavidus metallidurans strain CH34 is a multiple metal resistant -proteobacterium isolated from the sediments of a zinc metallurgical plant. This strain possesses a large diversity of heavy metal resistance genes that are located mostly on two large plasmids, pMOL28 and pMOL30. The metal efflux ATPases constitute the major mechanism by which metal ions are removed and pumped out from the cytoplasmic pool of thiol groups. The genome of C. metallidurans CH34 contains eight such P1-ATPases. This high number of ATPases, compared to the P1-ATPase content in other microorganisms, is a typical feature of the adaptation of C. metallidurans CH34 to metal-rich biotopes. We performed phylogenetic analysis, and used quantitative PCR to study the metal specific induction of the ATPases genes and, for some of them, their neighbouring genes. One of the ATPases, a homologue of the E. coli zntA gene, was characterized in detail, by analyzing the phenotypes of zntA mutants in different genetic backgrounds. In addition to the already reported sensitivity to Zn(II), Cd(II) and Pb(II), zntA mutants also displayed a higher sensitivity to Tl(I) and Bi(III), indicating that the ZntA protein might play role in the tolerance of very large cations.

KW - Cupriavidus metallidurans

KW - ralstonia

KW - P-type ATPase

KW - heavy metal

KW - resistance

KW - plasmids

KW - quantitative PCR

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_34242

UR - http://knowledgecentre.sckcen.be/so2/bibref/3776

U2 - 10.1080/03067310600583824

DO - 10.1080/03067310600583824

M3 - Article

VL - 86

SP - 677

EP - 692

JO - International Journal of Environmental Analytical Chemistry

JF - International Journal of Environmental Analytical Chemistry

SN - 1029-0397

IS - 9

ER -

ID: 102758