Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations

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Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations. / Belmans, Niels; Gilles, Liese; Piroska, Virag; Hedisu, Mihaela; Salmon, Benjamin; Baatout, Sarah; Lucas, Stéphane; Jacobs, Reinhilde; Lambrichts, Ivo; Moreels, Marjan.

In: Dentomaxillofacial Radiology, Vol. 48, No. 20180428, 20180428, 05.04.2019, p. 1-11.

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Belmans, N, Gilles, L, Piroska, V, Hedisu, M, Salmon, B, Baatout, S, Lucas, S, Jacobs, R, Lambrichts, I & Moreels, M 2019, 'Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations', Dentomaxillofacial Radiology, vol. 48, no. 20180428, 20180428, pp. 1-11. https://doi.org/10.1259/dmfr.20180428

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Belmans, Niels ; Gilles, Liese ; Piroska, Virag ; Hedisu, Mihaela ; Salmon, Benjamin ; Baatout, Sarah ; Lucas, Stéphane ; Jacobs, Reinhilde ; Lambrichts, Ivo ; Moreels, Marjan. / Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations. In: Dentomaxillofacial Radiology. 2019 ; Vol. 48, No. 20180428. pp. 1-11.

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@article{14c344982a1445fb80249598a39bc8a6,
title = "Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations",
abstract = "Objectives: Cone-beam CT (CBCT) is a medical imaging technique used in dental medicine. However, there are no conclusive data available indicating that exposure to X-ray doses used by CBCT are harmless. We aim, for the first time, to characterize the potential age-dependent cellular and subcellular effects related to exposure to CBCT imaging. Current objective is to describe and validate the protocol for characterization of cellular and subcellular changes after diagnostic CBCT. Methods: Development and validation of a dedicated two-part protocol: 1) assessing DNA double strand breaks (DSBs) in buccal mucosal (BM) cells and 2) oxidative stress measurements in saliva samples. BM cells and saliva samples are collected prior to and 0.5 h after CBCT examination. BM cells are also collected 24 h after CBCT examination. DNA DSBs are monitored in BM cells via immunocytochemical staining for γH2AX and 53BP1. 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dG) and total antioxidant capacity are measured in saliva to assess oxidative damage. Results: Validation experiments show that sufficient BM cells are collected (97.1 ± 1.4 {\%}) and that γH2AX/53BP1 foci can be detected before and after CBCT examination. Collection and analysis of saliva samples, either sham exposed or exposed to IR, show that changes in 8-oxo-dG and total antioxidant capacity can be detected in saliva samples after CBCT examination. Conclusion: The DIMITRA Research Group presents a two-part protocol to analyze potential age-related biological differences following CBCT examinations. This protocol was validated for collecting BM cells and saliva and for analyzing these samples for DNA DSBs and oxidative stress markers, respectively.",
keywords = "dental cone beam CT, DNA double strand breaks, Oxidative stress, buccal mucosal cells, saliva",
author = "Niels Belmans and Liese Gilles and Virag Piroska and Mihaela Hedisu and Benjamin Salmon and Sarah Baatout and St{\'e}phane Lucas and Reinhilde Jacobs and Ivo Lambrichts and Marjan Moreels",
note = "Score=10",
year = "2019",
month = "4",
day = "5",
doi = "10.1259/dmfr.20180428",
language = "English",
volume = "48",
pages = "1--11",
journal = "Dentomaxillofacial Radiology",
issn = "0250-832X",
publisher = "British Institute of Radiology",
number = "20180428",

}

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TY - JOUR

T1 - Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations

AU - Belmans, Niels

AU - Gilles, Liese

AU - Piroska, Virag

AU - Hedisu, Mihaela

AU - Salmon, Benjamin

AU - Baatout, Sarah

AU - Lucas, Stéphane

AU - Jacobs, Reinhilde

AU - Lambrichts, Ivo

AU - Moreels, Marjan

N1 - Score=10

PY - 2019/4/5

Y1 - 2019/4/5

N2 - Objectives: Cone-beam CT (CBCT) is a medical imaging technique used in dental medicine. However, there are no conclusive data available indicating that exposure to X-ray doses used by CBCT are harmless. We aim, for the first time, to characterize the potential age-dependent cellular and subcellular effects related to exposure to CBCT imaging. Current objective is to describe and validate the protocol for characterization of cellular and subcellular changes after diagnostic CBCT. Methods: Development and validation of a dedicated two-part protocol: 1) assessing DNA double strand breaks (DSBs) in buccal mucosal (BM) cells and 2) oxidative stress measurements in saliva samples. BM cells and saliva samples are collected prior to and 0.5 h after CBCT examination. BM cells are also collected 24 h after CBCT examination. DNA DSBs are monitored in BM cells via immunocytochemical staining for γH2AX and 53BP1. 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dG) and total antioxidant capacity are measured in saliva to assess oxidative damage. Results: Validation experiments show that sufficient BM cells are collected (97.1 ± 1.4 %) and that γH2AX/53BP1 foci can be detected before and after CBCT examination. Collection and analysis of saliva samples, either sham exposed or exposed to IR, show that changes in 8-oxo-dG and total antioxidant capacity can be detected in saliva samples after CBCT examination. Conclusion: The DIMITRA Research Group presents a two-part protocol to analyze potential age-related biological differences following CBCT examinations. This protocol was validated for collecting BM cells and saliva and for analyzing these samples for DNA DSBs and oxidative stress markers, respectively.

AB - Objectives: Cone-beam CT (CBCT) is a medical imaging technique used in dental medicine. However, there are no conclusive data available indicating that exposure to X-ray doses used by CBCT are harmless. We aim, for the first time, to characterize the potential age-dependent cellular and subcellular effects related to exposure to CBCT imaging. Current objective is to describe and validate the protocol for characterization of cellular and subcellular changes after diagnostic CBCT. Methods: Development and validation of a dedicated two-part protocol: 1) assessing DNA double strand breaks (DSBs) in buccal mucosal (BM) cells and 2) oxidative stress measurements in saliva samples. BM cells and saliva samples are collected prior to and 0.5 h after CBCT examination. BM cells are also collected 24 h after CBCT examination. DNA DSBs are monitored in BM cells via immunocytochemical staining for γH2AX and 53BP1. 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dG) and total antioxidant capacity are measured in saliva to assess oxidative damage. Results: Validation experiments show that sufficient BM cells are collected (97.1 ± 1.4 %) and that γH2AX/53BP1 foci can be detected before and after CBCT examination. Collection and analysis of saliva samples, either sham exposed or exposed to IR, show that changes in 8-oxo-dG and total antioxidant capacity can be detected in saliva samples after CBCT examination. Conclusion: The DIMITRA Research Group presents a two-part protocol to analyze potential age-related biological differences following CBCT examinations. This protocol was validated for collecting BM cells and saliva and for analyzing these samples for DNA DSBs and oxidative stress markers, respectively.

KW - dental cone beam CT

KW - DNA double strand breaks

KW - Oxidative stress

KW - buccal mucosal cells

KW - saliva

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/33584022

UR - https://www.ncbi.nlm.nih.gov/pubmed/30912976

U2 - 10.1259/dmfr.20180428

DO - 10.1259/dmfr.20180428

M3 - Article

VL - 48

SP - 1

EP - 11

JO - Dentomaxillofacial Radiology

JF - Dentomaxillofacial Radiology

SN - 0250-832X

IS - 20180428

M1 - 20180428

ER -

ID: 5057528