MiRNA398b and miRNA398c are involved in the regulation of the SOD response in uranium-exposed Arabidopsis thaliana roots

Research output: Contribution to journalArticle

Standard

MiRNA398b and miRNA398c are involved in the regulation of the SOD response in uranium-exposed Arabidopsis thaliana roots. / Saenen, Eline; Horemans, Nele; Vanhoudt, Nathalie; Vandenhove, Hildegarde; Biermans, Geert; Van Hees, May; Wannijn, Jean; Vangronsveld, Jaco; Cuypers, Ann.

In: Environmental and Experimental Botany, Vol. 116, 02.08.2015, p. 12-19.

Research output: Contribution to journalArticle

Author

Saenen, Eline; Horemans, Nele; Vanhoudt, Nathalie; Vandenhove, Hildegarde; Biermans, Geert; Van Hees, May; Wannijn, Jean; Vangronsveld, Jaco; Cuypers, Ann / MiRNA398b and miRNA398c are involved in the regulation of the SOD response in uranium-exposed Arabidopsis thaliana roots.

In: Environmental and Experimental Botany, Vol. 116, 02.08.2015, p. 12-19.

Research output: Contribution to journalArticle

Bibtex - Download

@article{e9828c2d3da34b0bb73e923e36096f14,
title = "MiRNA398b and miRNA398c are involved in the regulation of the SOD response in uranium-exposed Arabidopsis thaliana roots",
keywords = "Arabidopsis thaliana, Uranium toxicity, pH, Oxidative stress, MicroRNA398",
author = "Eline Saenen and Nele Horemans and Nathalie Vanhoudt and Hildegarde Vandenhove and Geert Biermans and {Van Hees}, May and Jean Wannijn and Jaco Vangronsveld and Ann Cuypers",
note = "Score=10",
year = "2015",
month = "8",
doi = "10.1016/j.envexpbot.2015.03.003",
volume = "116",
pages = "12--19",
journal = "Environmental and Experimental Botany",
issn = "0098-8472",
publisher = "Elsevier",

}

RIS - Download

TY - JOUR

T1 - MiRNA398b and miRNA398c are involved in the regulation of the SOD response in uranium-exposed Arabidopsis thaliana roots

AU - Saenen,Eline

AU - Horemans,Nele

AU - Vanhoudt,Nathalie

AU - Vandenhove,Hildegarde

AU - Biermans,Geert

AU - Van Hees,May

AU - Wannijn,Jean

AU - Vangronsveld,Jaco

AU - Cuypers,Ann

N1 - Score=10

PY - 2015/8/2

Y1 - 2015/8/2

N2 - The chemical speciation of uranium (U), and hence its toxicity, is strongly dependent on pH. However, oxidative stress responses after U exposure have mainly been investigated in Arabidopsis thaliana plants at pH 5.5, the ideal pH for growing plants in a hydroponic setup. As the pH of pore water can vary strongly, the aim of this study is to investigate oxidative stress responses induced in roots of A. thaliana plants exposed to different U concentrations at pH 4.5 and hence at a high free uraynl (UO22+) concentration. In addition to analyzing growth reduction, effects were analysed at enzyme, metabolite and genetic level. Results indicate that U is highly toxic at low pH resulting in a significant decrease in fresh weight (EC50 value for root growth reduction: 28.14 ± 1.59 μM U). In addition, no intact RNA could be extracted from the roots exposed to 75 and 100 μM U and the ascorbate concentrations could not be determined in roots exposed to 50, 75 or 100 μM U indicating that the roots are seriously damaged. Concerning the antioxidative defence system, the involvement of miRNA398b/c in the regulation of the superoxide dismutase (SOD) response was observed after U exposure. As such, a significant increase in MIR398b/c expression was observed, accompanied by a decreased copper/zinc SOD (CSD1/2) expression. The latter was compensated by an increased expression of iron SOD (FSD1) to maintain the SOD capacity in the plastids. While the involvement of miRNA398b/c was already reported before under copper or cadmium stress, this is the first time that it is reported for U.

AB - The chemical speciation of uranium (U), and hence its toxicity, is strongly dependent on pH. However, oxidative stress responses after U exposure have mainly been investigated in Arabidopsis thaliana plants at pH 5.5, the ideal pH for growing plants in a hydroponic setup. As the pH of pore water can vary strongly, the aim of this study is to investigate oxidative stress responses induced in roots of A. thaliana plants exposed to different U concentrations at pH 4.5 and hence at a high free uraynl (UO22+) concentration. In addition to analyzing growth reduction, effects were analysed at enzyme, metabolite and genetic level. Results indicate that U is highly toxic at low pH resulting in a significant decrease in fresh weight (EC50 value for root growth reduction: 28.14 ± 1.59 μM U). In addition, no intact RNA could be extracted from the roots exposed to 75 and 100 μM U and the ascorbate concentrations could not be determined in roots exposed to 50, 75 or 100 μM U indicating that the roots are seriously damaged. Concerning the antioxidative defence system, the involvement of miRNA398b/c in the regulation of the superoxide dismutase (SOD) response was observed after U exposure. As such, a significant increase in MIR398b/c expression was observed, accompanied by a decreased copper/zinc SOD (CSD1/2) expression. The latter was compensated by an increased expression of iron SOD (FSD1) to maintain the SOD capacity in the plastids. While the involvement of miRNA398b/c was already reported before under copper or cadmium stress, this is the first time that it is reported for U.

KW - Arabidopsis thaliana

KW - Uranium toxicity

KW - pH

KW - Oxidative stress

KW - MicroRNA398

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/33759352

U2 - 10.1016/j.envexpbot.2015.03.003

DO - 10.1016/j.envexpbot.2015.03.003

M3 - Article

VL - 116

SP - 12

EP - 19

JO - Environmental and Experimental Botany

T2 - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

SN - 0098-8472

ER -

ID: 5102379