Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells.

Research output: Contribution to journalArticlepeer-review

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Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells. / Infanger, Manfred; Ulbrich, Claudia; Baatout, Sarah; Wehland, Markus; Kreutz, Reinhold; Bauer, Johann; Grosse, Jirka; Vadrucci, Sonia; Cogoli, Augusto; Derradji, Hanane; Neefs, Mieke; Kusters, Sabine; Spain, Mike; Paul, Martin; Grimm, Daniela.

In: Journal of Cellular Biochemistry, Vol. 101, No. 6, 23.08.2007, p. 1439-1455.

Research output: Contribution to journalArticlepeer-review

Harvard

Infanger, M, Ulbrich, C, Baatout, S, Wehland, M, Kreutz, R, Bauer, J, Grosse, J, Vadrucci, S, Cogoli, A, Derradji, H, Neefs, M, Kusters, S, Spain, M, Paul, M & Grimm, D 2007, 'Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells.', Journal of Cellular Biochemistry, vol. 101, no. 6, pp. 1439-1455. https://doi.org/10.1002/jcb.21261

APA

Infanger, M., Ulbrich, C., Baatout, S., Wehland, M., Kreutz, R., Bauer, J., Grosse, J., Vadrucci, S., Cogoli, A., Derradji, H., Neefs, M., Kusters, S., Spain, M., Paul, M., & Grimm, D. (2007). Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells. Journal of Cellular Biochemistry, 101(6), 1439-1455. https://doi.org/10.1002/jcb.21261

Vancouver

Infanger M, Ulbrich C, Baatout S, Wehland M, Kreutz R, Bauer J et al. Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells. Journal of Cellular Biochemistry. 2007 Aug 23;101(6):1439-1455. https://doi.org/10.1002/jcb.21261

Author

Infanger, Manfred ; Ulbrich, Claudia ; Baatout, Sarah ; Wehland, Markus ; Kreutz, Reinhold ; Bauer, Johann ; Grosse, Jirka ; Vadrucci, Sonia ; Cogoli, Augusto ; Derradji, Hanane ; Neefs, Mieke ; Kusters, Sabine ; Spain, Mike ; Paul, Martin ; Grimm, Daniela. / Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells. In: Journal of Cellular Biochemistry. 2007 ; Vol. 101, No. 6. pp. 1439-1455.

Bibtex - Download

@article{74ab2d44aad04bf79476b79b869e3440,
title = "Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells.",
abstract = "Many space missions have shown that prolonged space flights may increase the risk of cardiovascular problems. Using a three-dimensional clinostat, we investigated human endothelial EA.hy926 cells up to 10 days under conditions of simulated microgravity to distinguish transient from long-term effects of 0g. Maximum expression of all selected genes occurred after 10 minutes of clinorotation. Gene expression (osteopontin, Fas, TGF-{\ss}) declined to slightly upregulated levels or rose again (caspase-3) after the fourth day of clinorotation. Caspase-3, Bax and Bcl-2 protein content was enhanced for 10 days of microgravity. In addition, long-term accumulation of collagen type I and III and alterations of the cytoskeletal alpha- and beta-tubulins and F-actin were detectable. A significantly reduced release of soluble factors in simulated microgravity was measured for brain-derived neurotrophic factor, tissue factor, VEGF and interestingly for endothelin-1, which is important in keeping cardiovascular balances. The gene expression of endothelin-1 was suppressed under 0g conditions at day 7 and 10. Alterations of the vascular endothelium together with a decreased release of endothelin-1 may entail post-flight health hazards for astronauts.",
keywords = "microgravity, space, endothelial cells",
author = "Manfred Infanger and Claudia Ulbrich and Sarah Baatout and Markus Wehland and Reinhold Kreutz and Johann Bauer and Jirka Grosse and Sonia Vadrucci and Augusto Cogoli and Hanane Derradji and Mieke Neefs and Sabine Kusters and Mike Spain and Martin Paul and Daniela Grimm",
note = "Score = 10",
year = "2007",
month = aug,
day = "23",
doi = "10.1002/jcb.21261",
language = "English",
volume = "101",
pages = "1439--1455",
journal = "Journal of Cellular Biochemistry",
issn = "1097-4644",
publisher = "Wiley - John Wiley & Sons, Ltd",
number = "6",

}

RIS - Download

TY - JOUR

T1 - Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells.

AU - Infanger, Manfred

AU - Ulbrich, Claudia

AU - Baatout, Sarah

AU - Wehland, Markus

AU - Kreutz, Reinhold

AU - Bauer, Johann

AU - Grosse, Jirka

AU - Vadrucci, Sonia

AU - Cogoli, Augusto

AU - Derradji, Hanane

AU - Neefs, Mieke

AU - Kusters, Sabine

AU - Spain, Mike

AU - Paul, Martin

AU - Grimm, Daniela

N1 - Score = 10

PY - 2007/8/23

Y1 - 2007/8/23

N2 - Many space missions have shown that prolonged space flights may increase the risk of cardiovascular problems. Using a three-dimensional clinostat, we investigated human endothelial EA.hy926 cells up to 10 days under conditions of simulated microgravity to distinguish transient from long-term effects of 0g. Maximum expression of all selected genes occurred after 10 minutes of clinorotation. Gene expression (osteopontin, Fas, TGF-ß) declined to slightly upregulated levels or rose again (caspase-3) after the fourth day of clinorotation. Caspase-3, Bax and Bcl-2 protein content was enhanced for 10 days of microgravity. In addition, long-term accumulation of collagen type I and III and alterations of the cytoskeletal alpha- and beta-tubulins and F-actin were detectable. A significantly reduced release of soluble factors in simulated microgravity was measured for brain-derived neurotrophic factor, tissue factor, VEGF and interestingly for endothelin-1, which is important in keeping cardiovascular balances. The gene expression of endothelin-1 was suppressed under 0g conditions at day 7 and 10. Alterations of the vascular endothelium together with a decreased release of endothelin-1 may entail post-flight health hazards for astronauts.

AB - Many space missions have shown that prolonged space flights may increase the risk of cardiovascular problems. Using a three-dimensional clinostat, we investigated human endothelial EA.hy926 cells up to 10 days under conditions of simulated microgravity to distinguish transient from long-term effects of 0g. Maximum expression of all selected genes occurred after 10 minutes of clinorotation. Gene expression (osteopontin, Fas, TGF-ß) declined to slightly upregulated levels or rose again (caspase-3) after the fourth day of clinorotation. Caspase-3, Bax and Bcl-2 protein content was enhanced for 10 days of microgravity. In addition, long-term accumulation of collagen type I and III and alterations of the cytoskeletal alpha- and beta-tubulins and F-actin were detectable. A significantly reduced release of soluble factors in simulated microgravity was measured for brain-derived neurotrophic factor, tissue factor, VEGF and interestingly for endothelin-1, which is important in keeping cardiovascular balances. The gene expression of endothelin-1 was suppressed under 0g conditions at day 7 and 10. Alterations of the vascular endothelium together with a decreased release of endothelin-1 may entail post-flight health hazards for astronauts.

KW - microgravity

KW - space

KW - endothelial cells

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_82289

UR - http://knowledgecentre.sckcen.be/so2/bibref/4377

U2 - 10.1002/jcb.21261

DO - 10.1002/jcb.21261

M3 - Article

VL - 101

SP - 1439

EP - 1455

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 1097-4644

IS - 6

ER -

ID: 126703