Modelled microgravity cultivation modulates N-acylhomoserine lactone production in Rhodospirillum rubrum S1H independently of cell density

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@article{f6023c2ca12c4d9680595271c549a9ff,
title = "Modelled microgravity cultivation modulates N-acylhomoserine lactone production in Rhodospirillum rubrum S1H independently of cell density",
abstract = "The photosynthetic alphaproteobacterium Rhodospirillum rubrum S1H is part of the Micro-Ecological Life Support System Alternative (MELiSSA) project that is aiming to develop a closed life support system for oxygen, water and food production to support human life in space in forthcoming long-term space exploration missions. In the present study, Rs. rubrum S1H was cultured in a rotating wall vessel (RWV), simulating partial microgravity conditions on Earth. The bacterium showed a significant response to cultivation in simulated microgravity at the transcriptomic, proteomic and metabolic levels. In simulated microgravity conditions three N-acyl-L-homoserine lactones (C10-HSL, C12-HSL and 3-OH-C14-HSL) were detected in concentrations that were twice those detected under normal gravity, while no differences in cell density was detected. In addition, Rs. rubrum cultivated in modelled microgravity showed higher pigmentation than the normal gravity control, without change in culture oxygenation. When compared to randomized microgravity cultivation using a random positioning machine (RPM), significant overlap for the top differentially expressed genes and proteins was observed. Cultivation in this new artificial environment of simulated microgravity showed new properties of this well-known bacterium including its first complete quorum-sensing-related N-acylhomoserine lactones profile.",
keywords = "Rhodospirillum rubrum, MELiSSA, Quorum sensing, rotating wall vessel, RWV, simulated microgravity",
author = "Felice Mastroleo and {Van Houdt}, Rob and Steve Atkison and Max Mergeay and Larissa Hendrickx and Ruddy Wattiez and Natalie Leys and {Condori Catachura}, Sandra",
note = "Score = 10",
year = "2013",
month = "9",
day = "11",
doi = "10.1099/mic.0.066415-0",
language = "English",
volume = "159",
pages = "2456--2466",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "10",

}

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TY - JOUR

T1 - Modelled microgravity cultivation modulates N-acylhomoserine lactone production in Rhodospirillum rubrum S1H independently of cell density

AU - Mastroleo, Felice

AU - Van Houdt, Rob

AU - Atkison, Steve

AU - Mergeay, Max

AU - Hendrickx, Larissa

AU - Wattiez, Ruddy

AU - Leys, Natalie

A2 - Condori Catachura, Sandra

N1 - Score = 10

PY - 2013/9/11

Y1 - 2013/9/11

N2 - The photosynthetic alphaproteobacterium Rhodospirillum rubrum S1H is part of the Micro-Ecological Life Support System Alternative (MELiSSA) project that is aiming to develop a closed life support system for oxygen, water and food production to support human life in space in forthcoming long-term space exploration missions. In the present study, Rs. rubrum S1H was cultured in a rotating wall vessel (RWV), simulating partial microgravity conditions on Earth. The bacterium showed a significant response to cultivation in simulated microgravity at the transcriptomic, proteomic and metabolic levels. In simulated microgravity conditions three N-acyl-L-homoserine lactones (C10-HSL, C12-HSL and 3-OH-C14-HSL) were detected in concentrations that were twice those detected under normal gravity, while no differences in cell density was detected. In addition, Rs. rubrum cultivated in modelled microgravity showed higher pigmentation than the normal gravity control, without change in culture oxygenation. When compared to randomized microgravity cultivation using a random positioning machine (RPM), significant overlap for the top differentially expressed genes and proteins was observed. Cultivation in this new artificial environment of simulated microgravity showed new properties of this well-known bacterium including its first complete quorum-sensing-related N-acylhomoserine lactones profile.

AB - The photosynthetic alphaproteobacterium Rhodospirillum rubrum S1H is part of the Micro-Ecological Life Support System Alternative (MELiSSA) project that is aiming to develop a closed life support system for oxygen, water and food production to support human life in space in forthcoming long-term space exploration missions. In the present study, Rs. rubrum S1H was cultured in a rotating wall vessel (RWV), simulating partial microgravity conditions on Earth. The bacterium showed a significant response to cultivation in simulated microgravity at the transcriptomic, proteomic and metabolic levels. In simulated microgravity conditions three N-acyl-L-homoserine lactones (C10-HSL, C12-HSL and 3-OH-C14-HSL) were detected in concentrations that were twice those detected under normal gravity, while no differences in cell density was detected. In addition, Rs. rubrum cultivated in modelled microgravity showed higher pigmentation than the normal gravity control, without change in culture oxygenation. When compared to randomized microgravity cultivation using a random positioning machine (RPM), significant overlap for the top differentially expressed genes and proteins was observed. Cultivation in this new artificial environment of simulated microgravity showed new properties of this well-known bacterium including its first complete quorum-sensing-related N-acylhomoserine lactones profile.

KW - Rhodospirillum rubrum

KW - MELiSSA

KW - Quorum sensing

KW - rotating wall vessel

KW - RWV

KW - simulated microgravity

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_131636

UR - http://knowledgecentre.sckcen.be/so2/bibref/10656

U2 - 10.1099/mic.0.066415-0

DO - 10.1099/mic.0.066415-0

M3 - Article

VL - 159

SP - 2456

EP - 2466

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 10

ER -

ID: 145709