Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation

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Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation. / Beck, Michaël; Rombouts, Charlotte; Aerts, An; Moreels, Marjan; Quintens, Roel; Tabury, Kevin; Michaux, Arlette; Janssen, Ann; Neefs, Mieke; Ernst, Eric; Dieriks, Birger; Lee, Ryonfa; De Vos, Winnok H.; Lambert, Charles; Van Oostveldt, Patrick; Baatout, Sarah; Vanmarcke, Hans (Peer reviewer).

In: International Journal of Molecular Medicine, Vol. 34, No. 4, 11.08.2014, p. 1124-1132.

Research output: Contribution to journalArticlepeer-review

Harvard

Beck, M, Rombouts, C, Aerts, A, Moreels, M, Quintens, R, Tabury, K, Michaux, A, Janssen, A, Neefs, M, Ernst, E, Dieriks, B, Lee, R, De Vos, WH, Lambert, C, Van Oostveldt, P, Baatout, S & Vanmarcke, H 2014, 'Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation', International Journal of Molecular Medicine, vol. 34, no. 4, pp. 1124-1132. https://doi.org/10.3892/ijmm.2014.1893

APA

Beck, M., Rombouts, C., Aerts, A., Moreels, M., Quintens, R., Tabury, K., Michaux, A., Janssen, A., Neefs, M., Ernst, E., Dieriks, B., Lee, R., De Vos, W. H., Lambert, C., Van Oostveldt, P., Baatout, S., & Vanmarcke, H. (2014). Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation. International Journal of Molecular Medicine, 34(4), 1124-1132. https://doi.org/10.3892/ijmm.2014.1893

Author

Beck, Michaël ; Rombouts, Charlotte ; Aerts, An ; Moreels, Marjan ; Quintens, Roel ; Tabury, Kevin ; Michaux, Arlette ; Janssen, Ann ; Neefs, Mieke ; Ernst, Eric ; Dieriks, Birger ; Lee, Ryonfa ; De Vos, Winnok H. ; Lambert, Charles ; Van Oostveldt, Patrick ; Baatout, Sarah ; Vanmarcke, Hans. / Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation. In: International Journal of Molecular Medicine. 2014 ; Vol. 34, No. 4. pp. 1124-1132.

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@article{810ce84248f9443182aa47469689127a,
title = "Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation",
abstract = "Ionizing radiation can elicit harmful effects on the cardiovascular system at high doses. Endothelial cells are critical targets in radiation-induced cardiovascular damage. Astronauts performing a long-term deep space mission are exposed to consistently higher fluences of ionizing radiation that may accumulate to reach high effective doses. In addition, cosmic radiation contains high linear energy transfer (LET) radiation that is known to produce high values of relative biological effectiveness (RBE). The aim of this study was to broaden the understanding of the molecular response to high LET radiation by investigating the changes in gene expression in endothelial cells. For this purpose, a human endothelial cell line (EA.hy926) was irradiated with accelerated nickel ions (Ni) (LET, 183 keV/μm) at doses of 0.5, 2 and 5 Gy. DNA damage was measured 2 and 24 h following irradiation by γ-H2AX foci detection by fluorescence microscopy and gene expression changes were measured by microarrays at 8 and 24 h following irradiation. We found that exposure to accelerated nickel particles induced a persistent DNA damage response up to 24 h after treatment.",
keywords = "gene expression, radiation, high-linear energy transfer, cardiovascular system, endothelial cells",
author = "Micha{\"e}l Beck and Charlotte Rombouts and An Aerts and Marjan Moreels and Roel Quintens and Kevin Tabury and Arlette Michaux and Ann Janssen and Mieke Neefs and Eric Ernst and Birger Dieriks and Ryonfa Lee and {De Vos}, {Winnok H.} and Charles Lambert and {Van Oostveldt}, Patrick and Sarah Baatout and Hans Vanmarcke",
note = "Score = 10",
year = "2014",
month = aug,
day = "11",
doi = "10.3892/ijmm.2014.1893",
language = "English",
volume = "34",
pages = "1124--1132",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "4",

}

RIS - Download

TY - JOUR

T1 - Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation

AU - Beck, Michaël

AU - Rombouts, Charlotte

AU - Aerts, An

AU - Moreels, Marjan

AU - Quintens, Roel

AU - Tabury, Kevin

AU - Michaux, Arlette

AU - Janssen, Ann

AU - Neefs, Mieke

AU - Ernst, Eric

AU - Dieriks, Birger

AU - Lee, Ryonfa

AU - De Vos, Winnok H.

AU - Lambert, Charles

AU - Van Oostveldt, Patrick

AU - Baatout, Sarah

A2 - Vanmarcke, Hans

N1 - Score = 10

PY - 2014/8/11

Y1 - 2014/8/11

N2 - Ionizing radiation can elicit harmful effects on the cardiovascular system at high doses. Endothelial cells are critical targets in radiation-induced cardiovascular damage. Astronauts performing a long-term deep space mission are exposed to consistently higher fluences of ionizing radiation that may accumulate to reach high effective doses. In addition, cosmic radiation contains high linear energy transfer (LET) radiation that is known to produce high values of relative biological effectiveness (RBE). The aim of this study was to broaden the understanding of the molecular response to high LET radiation by investigating the changes in gene expression in endothelial cells. For this purpose, a human endothelial cell line (EA.hy926) was irradiated with accelerated nickel ions (Ni) (LET, 183 keV/μm) at doses of 0.5, 2 and 5 Gy. DNA damage was measured 2 and 24 h following irradiation by γ-H2AX foci detection by fluorescence microscopy and gene expression changes were measured by microarrays at 8 and 24 h following irradiation. We found that exposure to accelerated nickel particles induced a persistent DNA damage response up to 24 h after treatment.

AB - Ionizing radiation can elicit harmful effects on the cardiovascular system at high doses. Endothelial cells are critical targets in radiation-induced cardiovascular damage. Astronauts performing a long-term deep space mission are exposed to consistently higher fluences of ionizing radiation that may accumulate to reach high effective doses. In addition, cosmic radiation contains high linear energy transfer (LET) radiation that is known to produce high values of relative biological effectiveness (RBE). The aim of this study was to broaden the understanding of the molecular response to high LET radiation by investigating the changes in gene expression in endothelial cells. For this purpose, a human endothelial cell line (EA.hy926) was irradiated with accelerated nickel ions (Ni) (LET, 183 keV/μm) at doses of 0.5, 2 and 5 Gy. DNA damage was measured 2 and 24 h following irradiation by γ-H2AX foci detection by fluorescence microscopy and gene expression changes were measured by microarrays at 8 and 24 h following irradiation. We found that exposure to accelerated nickel particles induced a persistent DNA damage response up to 24 h after treatment.

KW - gene expression

KW - radiation

KW - high-linear energy transfer

KW - cardiovascular system

KW - endothelial cells

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_137375

UR - http://knowledgecentre.sckcen.be/so2/bibref/11968

U2 - 10.3892/ijmm.2014.1893

DO - 10.3892/ijmm.2014.1893

M3 - Article

VL - 34

SP - 1124

EP - 1132

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 4

ER -

ID: 178199