Modulation of VEGF expression and oxidative stress response by iodine deficiency in irradiated cancerous and non-cancerous breast cells

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Modulation of VEGF expression and oxidative stress response by iodine deficiency in irradiated cancerous and non-cancerous breast cells. / Vanderstraeten, Jessica; Baselet, Bjorn; Buset, Jasmine; Ben Said, Naziha; de Ville de Goyet, Christine; Gérard, Anne-Catherine; Derradji, Hanane.

In: International Journal of Molecular Sciences, Vol. 21, No. 3963, 3963, 31.05.2020, p. 1-18.

Research output: Contribution to journalArticlepeer-review

Harvard

Vanderstraeten, J, Baselet, B, Buset, J, Ben Said, N, de Ville de Goyet, C, Gérard, A-C & Derradji, H 2020, 'Modulation of VEGF expression and oxidative stress response by iodine deficiency in irradiated cancerous and non-cancerous breast cells', International Journal of Molecular Sciences, vol. 21, no. 3963, 3963, pp. 1-18. https://doi.org/10.3390/ijms21113963

APA

Vanderstraeten, J., Baselet, B., Buset, J., Ben Said, N., de Ville de Goyet, C., Gérard, A-C., & Derradji, H. (2020). Modulation of VEGF expression and oxidative stress response by iodine deficiency in irradiated cancerous and non-cancerous breast cells. International Journal of Molecular Sciences, 21(3963), 1-18. [3963]. https://doi.org/10.3390/ijms21113963

Vancouver

Vanderstraeten J, Baselet B, Buset J, Ben Said N, de Ville de Goyet C, Gérard A-C et al. Modulation of VEGF expression and oxidative stress response by iodine deficiency in irradiated cancerous and non-cancerous breast cells. International Journal of Molecular Sciences. 2020 May 31;21(3963):1-18. 3963. https://doi.org/10.3390/ijms21113963

Author

Vanderstraeten, Jessica ; Baselet, Bjorn ; Buset, Jasmine ; Ben Said, Naziha ; de Ville de Goyet, Christine ; Gérard, Anne-Catherine ; Derradji, Hanane. / Modulation of VEGF expression and oxidative stress response by iodine deficiency in irradiated cancerous and non-cancerous breast cells. In: International Journal of Molecular Sciences. 2020 ; Vol. 21, No. 3963. pp. 1-18.

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@article{4dedded3a0794fdda067f2ad7b20d4ab,
title = "Modulation of VEGF expression and oxidative stress response by iodine deficiency in irradiated cancerous and non-cancerous breast cells",
abstract = "Breast cancer remains a major concern and its physiopathology is influenced by iodine deficiency (ID) and radiation exposure. Since radiation and ID can separately induce oxidative stress (OS) and microvascular responses in breast, their combination could additively increase these responses. Therefore, ID was induced in MCF7 and MCF12A breast cell lines by medium change. Cells were then X-irradiated with doses of 0.05, 0.1, or 3 Gy. In MCF12A cells, both ID and radiation (0.1 and 3 Gy) increased OS and vascular endothelial growth factor (VEGF) expression, with an additive effect when the highest dose was combined with ID. However, in MCF7 cells no additive effect was observed. VEGF mRNA up-regulation was reactive oxygen species (ROS)-dependent, involving radiation-induced mitochondrial ROS. Results on total VEGF mRNA hold true for the pro-angiogenic isoform VEGF165 mRNA, but the treatments did not modulate the anti-angiogenic isoform VEGF165b. Radiation-induced antioxidant response was differentially regulated upon ID in both cell lines. Thus, radiation response is modulated according to iodine status and cell type and can lead to additive effects on ROS and VEGF. As these are often involved in cancer initiation and progression, we believe that iodine status should be taken into account in radiation prevention policies",
keywords = "Radiation, Iodine deficiency, Breast, VEGF, ROS, Oxidative stress, MCF12A, MCF7",
author = "Jessica Vanderstraeten and Bjorn Baselet and Jasmine Buset and {Ben Said}, Naziha and {de Ville de Goyet}, Christine and Anne-Catherine G{\'e}rard and Hanane Derradji",
note = "Score=10",
year = "2020",
month = may,
day = "31",
doi = "10.3390/ijms21113963",
language = "English",
volume = "21",
pages = "1--18",
journal = "International Journal of Molecular Sciences",
issn = "1422-0067",
publisher = "MDPI",
number = "3963",

}

RIS - Download

TY - JOUR

T1 - Modulation of VEGF expression and oxidative stress response by iodine deficiency in irradiated cancerous and non-cancerous breast cells

AU - Vanderstraeten, Jessica

AU - Baselet, Bjorn

AU - Buset, Jasmine

AU - Ben Said, Naziha

AU - de Ville de Goyet, Christine

AU - Gérard, Anne-Catherine

AU - Derradji, Hanane

N1 - Score=10

PY - 2020/5/31

Y1 - 2020/5/31

N2 - Breast cancer remains a major concern and its physiopathology is influenced by iodine deficiency (ID) and radiation exposure. Since radiation and ID can separately induce oxidative stress (OS) and microvascular responses in breast, their combination could additively increase these responses. Therefore, ID was induced in MCF7 and MCF12A breast cell lines by medium change. Cells were then X-irradiated with doses of 0.05, 0.1, or 3 Gy. In MCF12A cells, both ID and radiation (0.1 and 3 Gy) increased OS and vascular endothelial growth factor (VEGF) expression, with an additive effect when the highest dose was combined with ID. However, in MCF7 cells no additive effect was observed. VEGF mRNA up-regulation was reactive oxygen species (ROS)-dependent, involving radiation-induced mitochondrial ROS. Results on total VEGF mRNA hold true for the pro-angiogenic isoform VEGF165 mRNA, but the treatments did not modulate the anti-angiogenic isoform VEGF165b. Radiation-induced antioxidant response was differentially regulated upon ID in both cell lines. Thus, radiation response is modulated according to iodine status and cell type and can lead to additive effects on ROS and VEGF. As these are often involved in cancer initiation and progression, we believe that iodine status should be taken into account in radiation prevention policies

AB - Breast cancer remains a major concern and its physiopathology is influenced by iodine deficiency (ID) and radiation exposure. Since radiation and ID can separately induce oxidative stress (OS) and microvascular responses in breast, their combination could additively increase these responses. Therefore, ID was induced in MCF7 and MCF12A breast cell lines by medium change. Cells were then X-irradiated with doses of 0.05, 0.1, or 3 Gy. In MCF12A cells, both ID and radiation (0.1 and 3 Gy) increased OS and vascular endothelial growth factor (VEGF) expression, with an additive effect when the highest dose was combined with ID. However, in MCF7 cells no additive effect was observed. VEGF mRNA up-regulation was reactive oxygen species (ROS)-dependent, involving radiation-induced mitochondrial ROS. Results on total VEGF mRNA hold true for the pro-angiogenic isoform VEGF165 mRNA, but the treatments did not modulate the anti-angiogenic isoform VEGF165b. Radiation-induced antioxidant response was differentially regulated upon ID in both cell lines. Thus, radiation response is modulated according to iodine status and cell type and can lead to additive effects on ROS and VEGF. As these are often involved in cancer initiation and progression, we believe that iodine status should be taken into account in radiation prevention policies

KW - Radiation

KW - Iodine deficiency

KW - Breast

KW - VEGF

KW - ROS

KW - Oxidative stress

KW - MCF12A

KW - MCF7

UR - https://ecm.sckcen.be/OTCS/llisapi.dll/open/38883403

U2 - 10.3390/ijms21113963

DO - 10.3390/ijms21113963

M3 - Article

VL - 21

SP - 1

EP - 18

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 3963

M1 - 3963

ER -

ID: 6824218