Molecular characterization of the life support bacterium Rhodospirillum rubrum S1H cultivated under space related environmental conditions

Research output: ThesisDoctoral thesis

Institutes & Expert groups

Abstract

R. rubrum S1H inoculated on solid agar medium in dark aerobic conditions was sent to the International space station. Moreover, in an effort to identify a specific response of R. rubrum to space flight, ground simulation of space ionizing radiation and space gravity were performed under identical culture setup and growth conditions encountered during the actual space flight experiment. The cultures were analysed and compared with their corresponding control at both the transcriptomic and proteomic level. In addition, R. rubrum whole protein content was further characterized using a multidimensional protein identification technology (MudPIT). We could put forward the importance of the medium composition and the culture setup on the response of the bacterium to the space flight related stress. We showed for the first time that a low dose of ionizing radiation (2 mGy) can induce a significant response at the transcriptomic level, although no change in cell viability and only a few significant differentially expressed proteins were observed. On the other hand, the MudPIT approach permitted us to identify about 25 % (i.e. 1,007 proteins) of R. rubrum's proteome.

Details

Original languageEnglish
Awarding Institution
  • UMons - University of Mons-Hainaut
Supervisors/Advisors
  • Wattiez, Ruddy, Supervisor, External person
  • Van Houdt, Rob, SCK•CEN Mentor
Place of PublicationMons, Belgium
Publisher
  • HELHa - Haute Ecole Louvain en Hainaut
Publication statusPublished - 23 Jan 2009

Keywords

  • Rhodospirillum rubrum S1H, transcriptomics, proteomics, space flight, ionizing radiation, random positioning machine, rotating wall vessel, MELiSSA

ID: 95178