Molecular investigation of radiation resistant cyanobacterium Arthrospira sp. PCC8005

Research output: ThesisDoctoral thesis

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Abstract

The cyanobacterium Arthrospira sp.PCC 8005 was selected by European Space Agency (ESA) for producing oxygen and food during future long-duration manned space missions, as part of the bioregenerative life support system 'MELiSSA'. For this task, it is essential that Arthrospira sp. PCC 8005 continues to produce oxygen and conserves high nutritive value while exposed to cosmic radiation in space. Our study showed that the Arthrospira cells are resistant to high doses of ionizing radiation, including both electromagnetic and particle irradiation. The live planktonic cells of Arthrospira sp. PCC 8005 are able to survive and fully recover their photosynthetic growth after exposure to doses of 6400 Gy of gamma irradiation, and 1000 and 2000 Gy of He and Fe particle radiation. Using a newly designed microarray chip and an optimized RNA extraction and protein analysis procedure, the molecular response of Arthrospira to high doses of gamma rays was investigated. These results, confirm that Arthrospira sp. PCC 8005 is valuable candidate for biotechnological applications in environments exposed to ionizing radiation, in space and on Earth.

Details

Original languageEnglish
Awarding Institution
  • UMons - University of Mons-Hainaut
Supervisors/Advisors
  • Leys, Natalie, Supervisor
  • Wattiez, Ruddy, Supervisor, External person
Place of PublicationMons, Belgium
Publisher
  • UMONS - Université de Mons
Publication statusPublished - 23 Jan 2015

Keywords

  • MELiSSA, Arthrospira, radiation

ID: 65474