Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005

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Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005. / Badri, Hanène; Monsieurs, Pieter; Coninx, Ilse; Wattiez, Ruddy; Leys, Natalie.

In: Microbiology Open, Vol. 4, No. 2, 04.2015, p. 187-207.

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@article{4948e38bb2d74683ba8d7052e57cb5f4,
title = "Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005",
abstract = "This study is the first, showing that Arthrospira is highly tolerant to gamma rays, and can survive at least 6400 Gy (dose rate of 527 Gy h _1), which identified Arthrospira sp. PCC 8005 as a radiation resistant bacterium. Biochemical, including proteomic and transcriptomic, analysis after irradiation with 3200 or 5000 Gy showed a decline in photosystem II quantum yield, reduced carbon fixation, and reduced pigment, lipid, and secondary metabolite synthesis. Transcription of photo-sensing and signalling pathways, and thiol-based antioxidant systems was induced. Transcriptomics did show significant activation of ssDNA repair systems and mobile genetic elements (MGEs) at the RNA level. Surprisingly, the cells did not induce the classical antioxidant or DNA repair systems, such superoxide dismutase (SOD) enzyme and the RecA protein. Arthrospira cells lack the catalase gene and the LexA repressor. Irradiated Arthrospira cells did induce strongly a group of conserved proteins, of which the function in radiation resistance remains to be elucidated, but which are a promising novel routes to be explored. This study revealed the radiation resistance of Arthrospira, and the molecular systems involved, paving the way for its further and better exploitation.",
keywords = "arthrospira, cyanobacteria, ionizing radiation, microarray, proteomics",
author = "Han{\`e}ne Badri and Pieter Monsieurs and Ilse Coninx and Ruddy Wattiez and Natalie Leys",
note = "Score = 10",
year = "2015",
month = apr,
doi = "10.1002/mbo3.229",
language = "English",
volume = "4",
pages = "187--207",
journal = "Microbiology Open",
issn = "2045-8827",
publisher = "Wiley - John Wiley & Sons, Ltd",
number = "2",

}

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TY - JOUR

T1 - Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005

AU - Badri, Hanène

AU - Monsieurs, Pieter

AU - Coninx, Ilse

AU - Wattiez, Ruddy

AU - Leys, Natalie

N1 - Score = 10

PY - 2015/4

Y1 - 2015/4

N2 - This study is the first, showing that Arthrospira is highly tolerant to gamma rays, and can survive at least 6400 Gy (dose rate of 527 Gy h _1), which identified Arthrospira sp. PCC 8005 as a radiation resistant bacterium. Biochemical, including proteomic and transcriptomic, analysis after irradiation with 3200 or 5000 Gy showed a decline in photosystem II quantum yield, reduced carbon fixation, and reduced pigment, lipid, and secondary metabolite synthesis. Transcription of photo-sensing and signalling pathways, and thiol-based antioxidant systems was induced. Transcriptomics did show significant activation of ssDNA repair systems and mobile genetic elements (MGEs) at the RNA level. Surprisingly, the cells did not induce the classical antioxidant or DNA repair systems, such superoxide dismutase (SOD) enzyme and the RecA protein. Arthrospira cells lack the catalase gene and the LexA repressor. Irradiated Arthrospira cells did induce strongly a group of conserved proteins, of which the function in radiation resistance remains to be elucidated, but which are a promising novel routes to be explored. This study revealed the radiation resistance of Arthrospira, and the molecular systems involved, paving the way for its further and better exploitation.

AB - This study is the first, showing that Arthrospira is highly tolerant to gamma rays, and can survive at least 6400 Gy (dose rate of 527 Gy h _1), which identified Arthrospira sp. PCC 8005 as a radiation resistant bacterium. Biochemical, including proteomic and transcriptomic, analysis after irradiation with 3200 or 5000 Gy showed a decline in photosystem II quantum yield, reduced carbon fixation, and reduced pigment, lipid, and secondary metabolite synthesis. Transcription of photo-sensing and signalling pathways, and thiol-based antioxidant systems was induced. Transcriptomics did show significant activation of ssDNA repair systems and mobile genetic elements (MGEs) at the RNA level. Surprisingly, the cells did not induce the classical antioxidant or DNA repair systems, such superoxide dismutase (SOD) enzyme and the RecA protein. Arthrospira cells lack the catalase gene and the LexA repressor. Irradiated Arthrospira cells did induce strongly a group of conserved proteins, of which the function in radiation resistance remains to be elucidated, but which are a promising novel routes to be explored. This study revealed the radiation resistance of Arthrospira, and the molecular systems involved, paving the way for its further and better exploitation.

KW - arthrospira

KW - cyanobacteria

KW - ionizing radiation

KW - microarray

KW - proteomics

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_140779

UR - http://knowledgecentre.sckcen.be/so2/bibref/13033

U2 - 10.1002/mbo3.229

DO - 10.1002/mbo3.229

M3 - Article

VL - 4

SP - 187

EP - 207

JO - Microbiology Open

JF - Microbiology Open

SN - 2045-8827

IS - 2

ER -

ID: 183048