Molecular Structure and Metal-binding Properties of the Periplasmic CopK Protein Expressed in Cupriavidus metallidurans CH34 During Copper Challenge

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Abstract

The copK gene is localized on the pMOL30 plasmid of Cupriavidus metallidurans CH34 within the complex cop cluster of genes, for which 21 genes have been identified. The expression of the corresponding periplasmic CopK protein is strongly upregulated in the presence of copper, leading to a high periplasmic accumulation. The structure and metal-binding properties of CopK were investigated by NMR and mass spectrometry.

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Original languageEnglish
Pages (from-to)386-403
JournalJournal of Molecular Biology
Volume380
Issue number2
DOIs
Publication statusPublished - 4 Jul 2008

Keywords

  • Cupriavidus metallidurans, mass spectrometry, nuclear magnetic resonance, protein structure, copper-resistance, plasmid pMOL30, methionine-residues

ID: 360879