Molecular Structure and Metal-binding Properties of the Periplasmic CopK Protein Expressed in Cupriavidus metallidurans CH34 During Copper Challenge
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Abstract
The copK gene is localized on the pMOL30 plasmid of Cupriavidus metallidurans
CH34 within the complex cop cluster of genes, for which 21 genes
have been identified. The expression of the corresponding periplasmic CopK
protein is strongly upregulated in the presence of copper, leading to a high
periplasmic accumulation. The structure and metal-binding properties of
CopK were investigated by NMR and mass spectrometry.
Details
| Original language | English |
|---|---|
| Pages (from-to) | 386-403 |
| Journal | Journal of Molecular Biology |
| Volume | 380 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 4 Jul 2008 |
Keywords
- Cupriavidus metallidurans, mass spectrometry, nuclear magnetic resonance, protein structure, copper-resistance, plasmid pMOL30, methionine-residues
ID: 360879