Molecular Structure and Metal-binding Properties of the Periplasmic CopK Protein Expressed in Cupriavidus metallidurans CH34 During Copper Challenge

Research output: Contribution to journal › Article

Authors

Institutes & Expert groups

Documents & links

DOI

Abstract

The copK gene is localized on the pMOL30 plasmid of Cupriavidus metallidurans CH34 within the complex cop cluster of genes, for which 21 genes have been identified. The expression of the corresponding periplasmic CopK protein is strongly upregulated in the presence of copper, leading to a high periplasmic accumulation. The structure and metal-binding properties of CopK were investigated by NMR and mass spectrometry.

Details

Original languageEnglish
Pages (from-to)386-403
JournalJournal of Molecular Biology
Volume380
Issue number2
DOIs
Publication statusPublished - 4 Jul 2008

Keywords

  • Cupriavidus metallidurans, mass spectrometry, nuclear magnetic resonance, protein structure, copper-resistance, plasmid pMOL30, methionine-residues

Related by author

  1. Cupriavidus metallidurans CH34, a historical perspective on its discovery, characterization and metal resistance

    Research output: Contribution to journal › Article

  2. Spontaneous mutation in the AgrRS two-component regulatory system of Cupriavidus metallidurans results in enhanced silver resistance

    Research output: Contribution to journal › Article

  3. Zinc-Induced Transposition of Insertion Sequence Elements Contributes to Increased Adaptability of Cupriavidus metallidurans

    Research output: Contribution to journal › Article

  4. Conjugative transfer of broad host range plasmids to an acidobacterial strain, Edaphobacter aggregans

    Research output: Contribution to journal › Article

  5. Metal Response in Cupriavidus metallidurans, Volume II: Insights into the Structure-Function Relationship of Proteins

    Research output: Report/book › Book as author

ID: 360879