Molecular Structure and Metal-binding Properties of the Periplasmic CopK Protein Expressed in Cupriavidus metallidurans CH34 During Copper Challenge

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Molecular Structure and Metal-binding Properties of the Periplasmic CopK Protein Expressed in Cupriavidus metallidurans CH34 During Copper Challenge. / Bersch, Beate; Favier, Adrien; Schanda, Paul; van Aelst, Sébastien; Vallaeys, Tatiana; Covès, Jacques; Mergeay, Max; Wattiez, Ruddy; Van Houdt, Rob (Peer reviewer).

In: Journal of Molecular Biology, Vol. 380, No. 2, 04.07.2008, p. 386-403.

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Bersch, Beate ; Favier, Adrien ; Schanda, Paul ; van Aelst, Sébastien ; Vallaeys, Tatiana ; Covès, Jacques ; Mergeay, Max ; Wattiez, Ruddy ; Van Houdt, Rob. / Molecular Structure and Metal-binding Properties of the Periplasmic CopK Protein Expressed in Cupriavidus metallidurans CH34 During Copper Challenge. In: Journal of Molecular Biology. 2008 ; Vol. 380, No. 2. pp. 386-403.

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@article{f63de03a5614454dbd0c7a740154df62,
title = "Molecular Structure and Metal-binding Properties of the Periplasmic CopK Protein Expressed in Cupriavidus metallidurans CH34 During Copper Challenge",
abstract = "The copK gene is localized on the pMOL30 plasmid of Cupriavidus metallidurans CH34 within the complex cop cluster of genes, for which 21 genes have been identified. The expression of the corresponding periplasmic CopK protein is strongly upregulated in the presence of copper, leading to a high periplasmic accumulation. The structure and metal-binding properties of CopK were investigated by NMR and mass spectrometry.",
keywords = "Cupriavidus metallidurans, mass spectrometry, nuclear magnetic resonance, protein structure, copper-resistance, plasmid pMOL30, methionine-residues",
author = "Beate Bersch and Adrien Favier and Paul Schanda and {van Aelst}, S{\'e}bastien and Tatiana Vallaeys and Jacques Cov{\`e}s and Max Mergeay and Ruddy Wattiez and {Van Houdt}, Rob",
note = "Score = 10",
year = "2008",
month = jul,
day = "4",
doi = "10.1016/j.jmb.2008.05.017",
language = "English",
volume = "380",
pages = "386--403",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Elsevier",
number = "2",

}

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TY - JOUR

T1 - Molecular Structure and Metal-binding Properties of the Periplasmic CopK Protein Expressed in Cupriavidus metallidurans CH34 During Copper Challenge

AU - Bersch, Beate

AU - Favier, Adrien

AU - Schanda, Paul

AU - van Aelst, Sébastien

AU - Vallaeys, Tatiana

AU - Covès, Jacques

AU - Mergeay, Max

AU - Wattiez, Ruddy

A2 - Van Houdt, Rob

N1 - Score = 10

PY - 2008/7/4

Y1 - 2008/7/4

N2 - The copK gene is localized on the pMOL30 plasmid of Cupriavidus metallidurans CH34 within the complex cop cluster of genes, for which 21 genes have been identified. The expression of the corresponding periplasmic CopK protein is strongly upregulated in the presence of copper, leading to a high periplasmic accumulation. The structure and metal-binding properties of CopK were investigated by NMR and mass spectrometry.

AB - The copK gene is localized on the pMOL30 plasmid of Cupriavidus metallidurans CH34 within the complex cop cluster of genes, for which 21 genes have been identified. The expression of the corresponding periplasmic CopK protein is strongly upregulated in the presence of copper, leading to a high periplasmic accumulation. The structure and metal-binding properties of CopK were investigated by NMR and mass spectrometry.

KW - Cupriavidus metallidurans

KW - mass spectrometry

KW - nuclear magnetic resonance

KW - protein structure

KW - copper-resistance

KW - plasmid pMOL30

KW - methionine-residues

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_95018

UR - http://knowledgecentre.sckcen.be/so2/bibref/5631

U2 - 10.1016/j.jmb.2008.05.017

DO - 10.1016/j.jmb.2008.05.017

M3 - Article

VL - 380

SP - 386

EP - 403

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 2

ER -

ID: 360879