Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy

Research output: Contribution to journalArticle

Authors

  • JD Gelder
  • D. Willemse-Erix
  • MJ Scholtes
  • JI Sanchez
  • K. Maquelin
  • P. Vandenabeele
  • Patrick De Boever
  • GJ Puppels
  • L. Moens
  • PD Vos

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DOI

Abstract

This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm-1 appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.

Details

Original languageEnglish
Pages (from-to)2155-2160
JournalAnalytical chemistry
Volume80
Issue number6
DOIs
Publication statusPublished - 15 Mar 2008

Keywords

  • Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy

ID: 62262