Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy

Research output: Contribution to journal › Article › peer-review

Standard

Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy. / Gelder, JD; Willemse-Erix, D.; Scholtes, MJ; Sanchez, JI; Maquelin, K.; Vandenabeele, P.; De Boever, Patrick; Puppels, GJ; Moens, L.; Vos, PD.

In: Analytical chemistry, Vol. 80, No. 6, 15.03.2008, p. 2155-2160.

Research output: Contribution to journal › Article › peer-review

Harvard

Gelder, JD, Willemse-Erix, D, Scholtes, MJ, Sanchez, JI, Maquelin, K, Vandenabeele, P, De Boever, P, Puppels, GJ, Moens, L & Vos, PD 2008, 'Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy', Analytical chemistry, vol. 80, no. 6, pp. 2155-2160. https://doi.org/10.1021/ac702185d

APA

Gelder, JD., Willemse-Erix, D., Scholtes, MJ., Sanchez, JI., Maquelin, K., Vandenabeele, P., De Boever, P., Puppels, GJ., Moens, L., & Vos, PD. (2008). Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy. Analytical chemistry, 80(6), 2155-2160. https://doi.org/10.1021/ac702185d

Vancouver

Gelder JD, Willemse-Erix D, Scholtes MJ, Sanchez JI, Maquelin K, Vandenabeele P et al. Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy. Analytical chemistry. 2008 Mar 15;80(6):2155-2160. https://doi.org/10.1021/ac702185d

Author

Gelder, JD ; Willemse-Erix, D. ; Scholtes, MJ ; Sanchez, JI ; Maquelin, K. ; Vandenabeele, P. ; De Boever, Patrick ; Puppels, GJ ; Moens, L. ; Vos, PD. / Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy. In: Analytical chemistry. 2008 ; Vol. 80, No. 6. pp. 2155-2160.

Bibtex - Download

@article{774d5a07d4614b2489f06845cf79e5dc,

title = "Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy",

abstract = "This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm-1 appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.",

keywords = "Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy",

author = "JD Gelder and D. Willemse-Erix and MJ Scholtes and JI Sanchez and K. Maquelin and P. Vandenabeele and {De Boever}, Patrick and GJ Puppels and L. Moens and PD Vos",

note = "Score = 10",

year = "2008",

month = mar,

day = "15",

doi = "10.1021/ac702185d",

language = "English",

volume = "80",

pages = "2155--2160",

journal = "Analytical chemistry",

issn = "0003-2700",

publisher = "ACS - American Chemical Society",

number = "6",

}

RIS - Download

TY - JOUR

T1 - Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy

AU - Gelder, JD

AU - Willemse-Erix, D.

AU - Scholtes, MJ

AU - Sanchez, JI

AU - Maquelin, K.

AU - Vandenabeele, P.

AU - De Boever, Patrick

AU - Puppels, GJ

AU - Moens, L.

AU - Vos, PD

N1 - Score = 10

PY - 2008/3/15

Y1 - 2008/3/15

N2 - This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm-1 appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.

AB - This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm-1 appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.

KW - Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_94930

UR - http://knowledgecentre.sckcen.be/so2/bibref/5604

U2 - 10.1021/ac702185d

DO - 10.1021/ac702185d

M3 - Article

VL - 80

SP - 2155

EP - 2160

JO - Analytical chemistry

JF - Analytical chemistry

SN - 0003-2700

IS - 6

ER -

ID: 62262