Morphological and Physiological Changes in Mature In Vitro Neuronal Networks towards Exposure to Short-, Middle- or Long-Term Simulated Microgravity

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@article{a41277ae331e45679fa16f9d5db111bc,
title = "Morphological and Physiological Changes in Mature In Vitro Neuronal Networks towards Exposure to Short-, Middle- or Long-Term Simulated Microgravity",
abstract = "The aim of this work was to assess the particular effects of simulated microgravity on mature primary neuronal networks and specially their plasticity and connectivity. For this purpose, primary mouse neuronal cultures (10 DIV) were subjected to microgravity using the Random Positioning Machine (RPM). These cultures were then used to investigate the impact of short- (1 h), middle- (24 h) and long-term (10 days) exposure to microgravity at the level of neurite network density, cell morphology and motility as well as cytoskeleton properties. Image processing analysis revealed different responses depending on the duration of the exposure. After short- and middle-term exposures to simulated microgravity, changes in neurite network, neuron morphology and viability were observed with significant alterations followed by fast recovery processes. Long exposure to simulated microgravity revealed a high adaptation of single neurons to the new gravity conditions as well as a partial adaptation of neuronal networks. However, neurons and neuronal networks exposed for long-term to simulated microgravity required longer recovery time to re-adapt to the ground gravity. In conclusion, a clear modulation in neuronal plasticity was evidenced through morphological and physiological changes in primary neuronal cultures during and after simulated microgravity exposure.",
keywords = "microgravity, in vitro, neurite network",
author = "Giuseppe Pani and Nada Samari and Roel Quintens and {de Saint-Georges}, Louis and MariAntonia Meloni and Sarah Baatout and {Van Oostveldt}, Patrick and Rafi Benotmane and Marjan Moreels",
note = "Score = 10",
year = "2013",
month = "9",
doi = "10.1371/journal.pone.0073857",
language = "English",
volume = "8",
pages = "e73857--e73857",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "PLOS - Public Library of Science",
number = "9",

}

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TY - JOUR

T1 - Morphological and Physiological Changes in Mature In Vitro Neuronal Networks towards Exposure to Short-, Middle- or Long-Term Simulated Microgravity

AU - Pani, Giuseppe

AU - Samari, Nada

AU - Quintens, Roel

AU - de Saint-Georges, Louis

AU - Meloni, MariAntonia

AU - Baatout, Sarah

AU - Van Oostveldt, Patrick

AU - Benotmane, Rafi

A2 - Moreels, Marjan

N1 - Score = 10

PY - 2013/9

Y1 - 2013/9

N2 - The aim of this work was to assess the particular effects of simulated microgravity on mature primary neuronal networks and specially their plasticity and connectivity. For this purpose, primary mouse neuronal cultures (10 DIV) were subjected to microgravity using the Random Positioning Machine (RPM). These cultures were then used to investigate the impact of short- (1 h), middle- (24 h) and long-term (10 days) exposure to microgravity at the level of neurite network density, cell morphology and motility as well as cytoskeleton properties. Image processing analysis revealed different responses depending on the duration of the exposure. After short- and middle-term exposures to simulated microgravity, changes in neurite network, neuron morphology and viability were observed with significant alterations followed by fast recovery processes. Long exposure to simulated microgravity revealed a high adaptation of single neurons to the new gravity conditions as well as a partial adaptation of neuronal networks. However, neurons and neuronal networks exposed for long-term to simulated microgravity required longer recovery time to re-adapt to the ground gravity. In conclusion, a clear modulation in neuronal plasticity was evidenced through morphological and physiological changes in primary neuronal cultures during and after simulated microgravity exposure.

AB - The aim of this work was to assess the particular effects of simulated microgravity on mature primary neuronal networks and specially their plasticity and connectivity. For this purpose, primary mouse neuronal cultures (10 DIV) were subjected to microgravity using the Random Positioning Machine (RPM). These cultures were then used to investigate the impact of short- (1 h), middle- (24 h) and long-term (10 days) exposure to microgravity at the level of neurite network density, cell morphology and motility as well as cytoskeleton properties. Image processing analysis revealed different responses depending on the duration of the exposure. After short- and middle-term exposures to simulated microgravity, changes in neurite network, neuron morphology and viability were observed with significant alterations followed by fast recovery processes. Long exposure to simulated microgravity revealed a high adaptation of single neurons to the new gravity conditions as well as a partial adaptation of neuronal networks. However, neurons and neuronal networks exposed for long-term to simulated microgravity required longer recovery time to re-adapt to the ground gravity. In conclusion, a clear modulation in neuronal plasticity was evidenced through morphological and physiological changes in primary neuronal cultures during and after simulated microgravity exposure.

KW - microgravity

KW - in vitro

KW - neurite network

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_134144

UR - http://knowledgecentre.sckcen.be/so2/bibref/11231

U2 - 10.1371/journal.pone.0073857

DO - 10.1371/journal.pone.0073857

M3 - Article

VL - 8

SP - e73857-e73857

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 9

ER -

ID: 213168