Morphological defects in pine trees after the Fukushima accident: studying the underlying mechanisms at a molecular level

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@phdthesis{2718baee5f1a42bfbafeb7106be811a8,
title = "Morphological defects in pine trees after the Fukushima accident: studying the underlying mechanisms at a molecular level",
abstract = "The Fukushima nuclear disaster released large quantities of radionuclides into the environment. This caused a widespread radiation contamination, affecting large regions of Japan. A high number of Pinus densiflora trees in contaminated areas displayed abnormal morphological characteristics, of which a loss of apical dominance was the most notable. In this project, the underlying mechanisms of radiation-induced loss of apical dominance are investigated by designing and testing primers for genes central to the maintenance of apical dominance such as phytohormone synthesis/transport, oxidative stress and DNA damage/repair. P. densiflora needles collected in the Fukushima Exclusion zone were used for analysis. After RNA extraction, efficiency and specificity of the primers were tested using real-time qPCR. We were able to provide potential primers for genes involved in phytohormone synthesis/transport and elevated ROS biomarkers. To fully understand the role of ionizing radiation in the loss of apical dominance, additional research is needed.",
keywords = "Pinus densiflora, auxin, cytokinin, ionizing radiation, apical dominance",
author = "Berry Abramidis and Eline Saenen and Robin Nauts and Nele Horemans and Ann Cuypers",
note = "Score=0",
year = "2020",
month = "4",
day = "10",
language = "English",
school = "Uhasselt - Hasselt University",

}

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TY - THES

T1 - Morphological defects in pine trees after the Fukushima accident: studying the underlying mechanisms at a molecular level

AU - Abramidis, Berry

AU - Saenen, Eline

AU - Nauts, Robin

AU - Horemans, Nele

AU - Cuypers, Ann

N1 - Score=0

PY - 2020/4/10

Y1 - 2020/4/10

N2 - The Fukushima nuclear disaster released large quantities of radionuclides into the environment. This caused a widespread radiation contamination, affecting large regions of Japan. A high number of Pinus densiflora trees in contaminated areas displayed abnormal morphological characteristics, of which a loss of apical dominance was the most notable. In this project, the underlying mechanisms of radiation-induced loss of apical dominance are investigated by designing and testing primers for genes central to the maintenance of apical dominance such as phytohormone synthesis/transport, oxidative stress and DNA damage/repair. P. densiflora needles collected in the Fukushima Exclusion zone were used for analysis. After RNA extraction, efficiency and specificity of the primers were tested using real-time qPCR. We were able to provide potential primers for genes involved in phytohormone synthesis/transport and elevated ROS biomarkers. To fully understand the role of ionizing radiation in the loss of apical dominance, additional research is needed.

AB - The Fukushima nuclear disaster released large quantities of radionuclides into the environment. This caused a widespread radiation contamination, affecting large regions of Japan. A high number of Pinus densiflora trees in contaminated areas displayed abnormal morphological characteristics, of which a loss of apical dominance was the most notable. In this project, the underlying mechanisms of radiation-induced loss of apical dominance are investigated by designing and testing primers for genes central to the maintenance of apical dominance such as phytohormone synthesis/transport, oxidative stress and DNA damage/repair. P. densiflora needles collected in the Fukushima Exclusion zone were used for analysis. After RNA extraction, efficiency and specificity of the primers were tested using real-time qPCR. We were able to provide potential primers for genes involved in phytohormone synthesis/transport and elevated ROS biomarkers. To fully understand the role of ionizing radiation in the loss of apical dominance, additional research is needed.

KW - Pinus densiflora

KW - auxin

KW - cytokinin

KW - ionizing radiation

KW - apical dominance

UR - https://ecm.sckcen.be/OTCS/llisapi.dll/open/39525485

M3 - Master's thesis

ER -

ID: 6882479