Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans

Research output: Contribution to journalArticle

Authors

  • Max Mergeay
  • Catherine Tricot
  • Sébastien van Aelst
  • Ruddy Wattiez
  • Victor Stalon
  • Johan Wouters

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Abstract

CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A ˚ , and diffract to 2.2 A ˚ resolution using synchrotron radiation.

Details

Original languageEnglish
Pages (from-to)825-827
JournalActa Crystallographica Section F
Volume61
Issue number9
DOIs
Publication statusPublished - Aug 2005

Keywords

  • crystallography, copper resistance, protein, Cupriavidus metallidurans

ID: 296039