Oxidative stress responses induced by uranium exposure at low pH in leaves of Arabidopsis thaliana plants

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Oxidative stress responses induced by uranium exposure at low pH in leaves of Arabidopsis thaliana plants. / Saenen, Eline; Horemans, Nele; Vanhoudt, Nathalie; Vandenhove, Hildegarde; Biermans, Geert; Van Hees, May; Wannijn, Jean; Vangronsveld, Jaco; Cuypers, Ann.

In: Journal of environmental radioactivity, Vol. 150, 08.08.2015, p. 36-43.

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@article{76ecc223369f4735be0025276b2e3487,
title = "Oxidative stress responses induced by uranium exposure at low pH in leaves of Arabidopsis thaliana plants",
abstract = "Anthropogenic activities have led to a widespread uranium (U) contamination in many countries. The toxic effects of U at the cellular level have mainly been investigated at a pH around 5.5, the optimal pH for hydroponically grown plants. However, since the speciation of U, and hence its toxicity, is strongly dependent on environmental factors such as the pH, it is important to investigate the effects of U at different environmentally relevant pH levels. Although U is poorly translocated from the roots to the shoots, resulting in a low U concentration in the leaves, it has been demonstrated that toxic effects in the leaves were already visible after 1 day exposure at pH 5.5, although only when exposed to relatively high U concentrations (100 mM). Therefore, the present study aimed to analyse the effects of different U concentrations (ranging from 0 to 100 mM) at pH 4.5 in leaves of Arabidopsis thaliana plants. Results indicate that U induces early senescence in A. thaliana leaves as was suggested by a decreased expression of CAT2 accompanied by an induction of CAT3 expression, a decreased CAT capacity and an increased lipid peroxidation. In addition, miRNA398b/c is involved in the regulation of the SOD response in the leaves. As such, an increased MIR398b/c expression was observed leading to a decreased transcript level of CSD1/ 2. Finally, the biosynthesis of ascorbate was induced after U exposure. This can point towards an important role for this metabolite in the scavenging of reactive oxygen species under U stress.",
keywords = "Arabidopsis thaliana, Uranium toxicity, pH, Oxidative stress, Senescence",
author = "Eline Saenen and Nele Horemans and Nathalie Vanhoudt and Hildegarde Vandenhove and Geert Biermans and {Van Hees}, May and Jean Wannijn and Jaco Vangronsveld and Ann Cuypers",
note = "Score=10",
year = "2015",
month = "8",
day = "8",
doi = "10.1016/j.jenvrad.2015.07.021",
language = "English",
volume = "150",
pages = "36--43",
journal = "Journal of environmental radioactivity",
issn = "0265-931X",
publisher = "Elsevier",

}

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TY - JOUR

T1 - Oxidative stress responses induced by uranium exposure at low pH in leaves of Arabidopsis thaliana plants

AU - Saenen, Eline

AU - Horemans, Nele

AU - Vanhoudt, Nathalie

AU - Vandenhove, Hildegarde

AU - Biermans, Geert

AU - Van Hees, May

AU - Wannijn, Jean

AU - Vangronsveld, Jaco

AU - Cuypers, Ann

N1 - Score=10

PY - 2015/8/8

Y1 - 2015/8/8

N2 - Anthropogenic activities have led to a widespread uranium (U) contamination in many countries. The toxic effects of U at the cellular level have mainly been investigated at a pH around 5.5, the optimal pH for hydroponically grown plants. However, since the speciation of U, and hence its toxicity, is strongly dependent on environmental factors such as the pH, it is important to investigate the effects of U at different environmentally relevant pH levels. Although U is poorly translocated from the roots to the shoots, resulting in a low U concentration in the leaves, it has been demonstrated that toxic effects in the leaves were already visible after 1 day exposure at pH 5.5, although only when exposed to relatively high U concentrations (100 mM). Therefore, the present study aimed to analyse the effects of different U concentrations (ranging from 0 to 100 mM) at pH 4.5 in leaves of Arabidopsis thaliana plants. Results indicate that U induces early senescence in A. thaliana leaves as was suggested by a decreased expression of CAT2 accompanied by an induction of CAT3 expression, a decreased CAT capacity and an increased lipid peroxidation. In addition, miRNA398b/c is involved in the regulation of the SOD response in the leaves. As such, an increased MIR398b/c expression was observed leading to a decreased transcript level of CSD1/ 2. Finally, the biosynthesis of ascorbate was induced after U exposure. This can point towards an important role for this metabolite in the scavenging of reactive oxygen species under U stress.

AB - Anthropogenic activities have led to a widespread uranium (U) contamination in many countries. The toxic effects of U at the cellular level have mainly been investigated at a pH around 5.5, the optimal pH for hydroponically grown plants. However, since the speciation of U, and hence its toxicity, is strongly dependent on environmental factors such as the pH, it is important to investigate the effects of U at different environmentally relevant pH levels. Although U is poorly translocated from the roots to the shoots, resulting in a low U concentration in the leaves, it has been demonstrated that toxic effects in the leaves were already visible after 1 day exposure at pH 5.5, although only when exposed to relatively high U concentrations (100 mM). Therefore, the present study aimed to analyse the effects of different U concentrations (ranging from 0 to 100 mM) at pH 4.5 in leaves of Arabidopsis thaliana plants. Results indicate that U induces early senescence in A. thaliana leaves as was suggested by a decreased expression of CAT2 accompanied by an induction of CAT3 expression, a decreased CAT capacity and an increased lipid peroxidation. In addition, miRNA398b/c is involved in the regulation of the SOD response in the leaves. As such, an increased MIR398b/c expression was observed leading to a decreased transcript level of CSD1/ 2. Finally, the biosynthesis of ascorbate was induced after U exposure. This can point towards an important role for this metabolite in the scavenging of reactive oxygen species under U stress.

KW - Arabidopsis thaliana

KW - Uranium toxicity

KW - pH

KW - Oxidative stress

KW - Senescence

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/12037568

U2 - 10.1016/j.jenvrad.2015.07.021

DO - 10.1016/j.jenvrad.2015.07.021

M3 - Article

VL - 150

SP - 36

EP - 43

JO - Journal of environmental radioactivity

JF - Journal of environmental radioactivity

SN - 0265-931X

ER -

ID: 875411