Plasmids captured in C. metallidurans CH34: defining the PromA family of broad-host-range plasmids

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Plasmids captured in C. metallidurans CH34: defining the PromA family of broad-host-range plasmids. / Van der Auwera, Géraldine A.; Krol, Jaroslaw E.; Suzuki, Haruo; Foster, Brian; Van Houdt, Rob; Brown, Celeste J.; Mergeay, Max; Top, Eva M.

In: Antonie van Leeuwenhoek, Vol. 96, No. 2, 08.2009, p. 193-204.

Research output: Contribution to journalArticle

Harvard

Van der Auwera, GA, Krol, JE, Suzuki, H, Foster, B, Van Houdt, R, Brown, CJ, Mergeay, M & Top, EM 2009, 'Plasmids captured in C. metallidurans CH34: defining the PromA family of broad-host-range plasmids', Antonie van Leeuwenhoek, vol. 96, no. 2, pp. 193-204. https://doi.org/10.1007/s10482-009-9316-9

APA

Van der Auwera, G. A., Krol, J. E., Suzuki, H., Foster, B., Van Houdt, R., Brown, C. J., ... Top, E. M. (2009). Plasmids captured in C. metallidurans CH34: defining the PromA family of broad-host-range plasmids. Antonie van Leeuwenhoek, 96(2), 193-204. https://doi.org/10.1007/s10482-009-9316-9

Vancouver

Van der Auwera GA, Krol JE, Suzuki H, Foster B, Van Houdt R, Brown CJ et al. Plasmids captured in C. metallidurans CH34: defining the PromA family of broad-host-range plasmids. Antonie van Leeuwenhoek. 2009 Aug;96(2):193-204. https://doi.org/10.1007/s10482-009-9316-9

Author

Van der Auwera, Géraldine A. ; Krol, Jaroslaw E. ; Suzuki, Haruo ; Foster, Brian ; Van Houdt, Rob ; Brown, Celeste J. ; Mergeay, Max ; Top, Eva M. / Plasmids captured in C. metallidurans CH34: defining the PromA family of broad-host-range plasmids. In: Antonie van Leeuwenhoek. 2009 ; Vol. 96, No. 2. pp. 193-204.

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@article{553d2aef129f4dfeb4aa5e7d1d5941fa,
title = "Plasmids captured in C. metallidurans CH34: defining the PromA family of broad-host-range plasmids",
abstract = "The self-transmissible, broad-host-range (BHR) plasmid pMOL98 was previously isolated from polluted soil using a triparental plasmid capture approach and shown to possess a replicon similar to that of the BHR plasmids pSB102 and pIPO2. Here, complete sequence analysis and comparative genomics reveal that the 55.5 kb nucleotide sequence of pMOL98 shows extensive sequence similarity and synteny with the BHR plasmid family that now includes pIPO2, pSB102, pTER331, and pMRAD02. They share a plasmid backbone comprising replication, partitioning and conjugative transfer functions. Comparison of the variable accessory regions of these plasmids shows that the majority of natural transposons, as well as the mini-transposon used to mark the plasmids, are inserted in the parA locus. The transposon unique to pMOL98 appears to have inserted from the chromosome of the recipient strain used in the plasmid capture procedure. This demonstrates the necessity for careful screening of plasmids and host chromosomes to avoid mis-interpretation of plasmid genome content. The presence of very similar BHR plasmids with different accessory genes in geographically distinct locations suggests an important role in horizontal gene exchange and bacterial adaptation for this recently defined plasmid group, which we propose to name {"}PromA{"}.",
keywords = "plasmid, horizontal gene transfer, broad host range, transposon",
author = "{Van der Auwera}, {G{\'e}raldine A.} and Krol, {Jaroslaw E.} and Haruo Suzuki and Brian Foster and {Van Houdt}, Rob and Brown, {Celeste J.} and Max Mergeay and Top, {Eva M.}",
note = "Score = 10",
year = "2009",
month = "8",
doi = "10.1007/s10482-009-9316-9",
language = "English",
volume = "96",
pages = "193--204",
journal = "Antonie van Leeuwenhoek",
issn = "0003-6072",
publisher = "Springer",
number = "2",

}

RIS - Download

TY - JOUR

T1 - Plasmids captured in C. metallidurans CH34: defining the PromA family of broad-host-range plasmids

AU - Van der Auwera, Géraldine A.

AU - Krol, Jaroslaw E.

AU - Suzuki, Haruo

AU - Foster, Brian

AU - Van Houdt, Rob

AU - Brown, Celeste J.

AU - Mergeay, Max

AU - Top, Eva M.

N1 - Score = 10

PY - 2009/8

Y1 - 2009/8

N2 - The self-transmissible, broad-host-range (BHR) plasmid pMOL98 was previously isolated from polluted soil using a triparental plasmid capture approach and shown to possess a replicon similar to that of the BHR plasmids pSB102 and pIPO2. Here, complete sequence analysis and comparative genomics reveal that the 55.5 kb nucleotide sequence of pMOL98 shows extensive sequence similarity and synteny with the BHR plasmid family that now includes pIPO2, pSB102, pTER331, and pMRAD02. They share a plasmid backbone comprising replication, partitioning and conjugative transfer functions. Comparison of the variable accessory regions of these plasmids shows that the majority of natural transposons, as well as the mini-transposon used to mark the plasmids, are inserted in the parA locus. The transposon unique to pMOL98 appears to have inserted from the chromosome of the recipient strain used in the plasmid capture procedure. This demonstrates the necessity for careful screening of plasmids and host chromosomes to avoid mis-interpretation of plasmid genome content. The presence of very similar BHR plasmids with different accessory genes in geographically distinct locations suggests an important role in horizontal gene exchange and bacterial adaptation for this recently defined plasmid group, which we propose to name "PromA".

AB - The self-transmissible, broad-host-range (BHR) plasmid pMOL98 was previously isolated from polluted soil using a triparental plasmid capture approach and shown to possess a replicon similar to that of the BHR plasmids pSB102 and pIPO2. Here, complete sequence analysis and comparative genomics reveal that the 55.5 kb nucleotide sequence of pMOL98 shows extensive sequence similarity and synteny with the BHR plasmid family that now includes pIPO2, pSB102, pTER331, and pMRAD02. They share a plasmid backbone comprising replication, partitioning and conjugative transfer functions. Comparison of the variable accessory regions of these plasmids shows that the majority of natural transposons, as well as the mini-transposon used to mark the plasmids, are inserted in the parA locus. The transposon unique to pMOL98 appears to have inserted from the chromosome of the recipient strain used in the plasmid capture procedure. This demonstrates the necessity for careful screening of plasmids and host chromosomes to avoid mis-interpretation of plasmid genome content. The presence of very similar BHR plasmids with different accessory genes in geographically distinct locations suggests an important role in horizontal gene exchange and bacterial adaptation for this recently defined plasmid group, which we propose to name "PromA".

KW - plasmid

KW - horizontal gene transfer

KW - broad host range

KW - transposon

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_98405

UR - http://knowledgecentre.sckcen.be/so2/bibref/5922

U2 - 10.1007/s10482-009-9316-9

DO - 10.1007/s10482-009-9316-9

M3 - Article

VL - 96

SP - 193

EP - 204

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

SN - 0003-6072

IS - 2

ER -

ID: 187891