Properties of vasoactive‐intestinal‐peptide receptors and β‐adrenoceptors in the murine radiation leukemia‐virus‐induced lymphoma cell line BL/VL3

Research output: Contribution to journalArticle

Authors

  • J. Abello
  • C. Damien
  • Philippe De Neef
  • M. Tastenoy
  • Robert Hooghe
  • P. Robberecht
  • J. Christophe

Institutes & Expert groups

  • ULB - Université Libre de Bruxelles

Documents & links

Abstract

1. Based on radioligand binding and adenylate cyclase activation, functional receptors to vasoactive intestinal peptide(VIP)/helodermin, were shown to coexist with beta 2-adrenoceptors and prostaglandin receptors in membranes from a cultured cloned BL/VL3 cell line of murine T-cell lymphoma induced by a radiation leukemia virus. 2. The relative potency of VIP-related peptides to stimulate adenylate cyclase activity was: helodermin greater than VIP greater than peptide histidine isoleucinamide. Five VIP analogs inhibited 125I-iodo-VIP binding and stimulated adenylate cyclase activity, their decreasing order of potency being: VIP greater than [D-Asp3]VIP greater than [D-Ser2]VIP greater than [D-Ala4]VIP = [D-His1]VIP = [D-Phe2]VIP. [D-Phe2]VIP acted as a partial agonist (with an intrinsic activity of 0.1 as compared to that of VIP = 1.0) and competitively inhibited helodermin- and VIP-stimulated adenylate cyclase activity with a similar Ki (0.07-0.10 microM). These data suggest the existence, in this murine T-cell lymphoma, of VIP receptors of the 'helodermin-preferring' subtype that are coupled to adenylate cyclase.

Details

Original languageEnglish
Pages (from-to)263-267
Number of pages5
JournalEuropean Journal of Biochemistry
Volume183
Issue number2
DOIs
Publication statusPublished - 1 Aug 1989

ID: 4785321