Properties of vasoactive‐intestinal‐peptide receptors and β‐adrenoceptors in the murine radiation leukemia‐virus‐induced lymphoma cell line BL/VL3

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Properties of vasoactive‐intestinal‐peptide receptors and β‐adrenoceptors in the murine radiation leukemia‐virus‐induced lymphoma cell line BL/VL3. / Abello, J.; Damien, C.; De Neef, Philippe; Tastenoy, M.; Hooghe, Robert; Robberecht, P.; Christophe, J.

In: European Journal of Biochemistry, Vol. 183, No. 2, 01.08.1989, p. 263-267.

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Abello, J, Damien, C, De Neef, P, Tastenoy, M, Hooghe, R, Robberecht, P & Christophe, J 1989, 'Properties of vasoactive‐intestinal‐peptide receptors and β‐adrenoceptors in the murine radiation leukemia‐virus‐induced lymphoma cell line BL/VL3', European Journal of Biochemistry, vol. 183, no. 2, pp. 263-267. https://doi.org/10.1111/j.1432-1033.1989.tb14922.x

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Abello, J. ; Damien, C. ; De Neef, Philippe ; Tastenoy, M. ; Hooghe, Robert ; Robberecht, P. ; Christophe, J. / Properties of vasoactive‐intestinal‐peptide receptors and β‐adrenoceptors in the murine radiation leukemia‐virus‐induced lymphoma cell line BL/VL3. In: European Journal of Biochemistry. 1989 ; Vol. 183, No. 2. pp. 263-267.

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@article{872960005b124e1a9501f217326f21ee,
title = "Properties of vasoactive‐intestinal‐peptide receptors and β‐adrenoceptors in the murine radiation leukemia‐virus‐induced lymphoma cell line BL/VL3",
abstract = "1. Based on radioligand binding and adenylate cyclase activation, functional receptors to vasoactive intestinal peptide(VIP)/helodermin, were shown to coexist with beta 2-adrenoceptors and prostaglandin receptors in membranes from a cultured cloned BL/VL3 cell line of murine T-cell lymphoma induced by a radiation leukemia virus. 2. The relative potency of VIP-related peptides to stimulate adenylate cyclase activity was: helodermin greater than VIP greater than peptide histidine isoleucinamide. Five VIP analogs inhibited 125I-iodo-VIP binding and stimulated adenylate cyclase activity, their decreasing order of potency being: VIP greater than [D-Asp3]VIP greater than [D-Ser2]VIP greater than [D-Ala4]VIP = [D-His1]VIP = [D-Phe2]VIP. [D-Phe2]VIP acted as a partial agonist (with an intrinsic activity of 0.1 as compared to that of VIP = 1.0) and competitively inhibited helodermin- and VIP-stimulated adenylate cyclase activity with a similar Ki (0.07-0.10 microM). These data suggest the existence, in this murine T-cell lymphoma, of VIP receptors of the 'helodermin-preferring' subtype that are coupled to adenylate cyclase.",
author = "J. Abello and C. Damien and {De Neef}, Philippe and M. Tastenoy and Robert Hooghe and P. Robberecht and J. Christophe",
year = "1989",
month = "8",
day = "1",
doi = "10.1111/j.1432-1033.1989.tb14922.x",
language = "English",
volume = "183",
pages = "263--267",
journal = "European Journal of Biochemistry",
issn = "0014-2956",
publisher = "Wiley - John Wiley & Sons, Ltd",
number = "2",

}

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TY - JOUR

T1 - Properties of vasoactive‐intestinal‐peptide receptors and β‐adrenoceptors in the murine radiation leukemia‐virus‐induced lymphoma cell line BL/VL3

AU - Abello, J.

AU - Damien, C.

AU - De Neef, Philippe

AU - Tastenoy, M.

AU - Hooghe, Robert

AU - Robberecht, P.

AU - Christophe, J.

PY - 1989/8/1

Y1 - 1989/8/1

N2 - 1. Based on radioligand binding and adenylate cyclase activation, functional receptors to vasoactive intestinal peptide(VIP)/helodermin, were shown to coexist with beta 2-adrenoceptors and prostaglandin receptors in membranes from a cultured cloned BL/VL3 cell line of murine T-cell lymphoma induced by a radiation leukemia virus. 2. The relative potency of VIP-related peptides to stimulate adenylate cyclase activity was: helodermin greater than VIP greater than peptide histidine isoleucinamide. Five VIP analogs inhibited 125I-iodo-VIP binding and stimulated adenylate cyclase activity, their decreasing order of potency being: VIP greater than [D-Asp3]VIP greater than [D-Ser2]VIP greater than [D-Ala4]VIP = [D-His1]VIP = [D-Phe2]VIP. [D-Phe2]VIP acted as a partial agonist (with an intrinsic activity of 0.1 as compared to that of VIP = 1.0) and competitively inhibited helodermin- and VIP-stimulated adenylate cyclase activity with a similar Ki (0.07-0.10 microM). These data suggest the existence, in this murine T-cell lymphoma, of VIP receptors of the 'helodermin-preferring' subtype that are coupled to adenylate cyclase.

AB - 1. Based on radioligand binding and adenylate cyclase activation, functional receptors to vasoactive intestinal peptide(VIP)/helodermin, were shown to coexist with beta 2-adrenoceptors and prostaglandin receptors in membranes from a cultured cloned BL/VL3 cell line of murine T-cell lymphoma induced by a radiation leukemia virus. 2. The relative potency of VIP-related peptides to stimulate adenylate cyclase activity was: helodermin greater than VIP greater than peptide histidine isoleucinamide. Five VIP analogs inhibited 125I-iodo-VIP binding and stimulated adenylate cyclase activity, their decreasing order of potency being: VIP greater than [D-Asp3]VIP greater than [D-Ser2]VIP greater than [D-Ala4]VIP = [D-His1]VIP = [D-Phe2]VIP. [D-Phe2]VIP acted as a partial agonist (with an intrinsic activity of 0.1 as compared to that of VIP = 1.0) and competitively inhibited helodermin- and VIP-stimulated adenylate cyclase activity with a similar Ki (0.07-0.10 microM). These data suggest the existence, in this murine T-cell lymphoma, of VIP receptors of the 'helodermin-preferring' subtype that are coupled to adenylate cyclase.

UR - https://ecm.sckcen.be/OTCS/llisapi.dll?func=ll&objaction=overview&objid=32316260

U2 - 10.1111/j.1432-1033.1989.tb14922.x

DO - 10.1111/j.1432-1033.1989.tb14922.x

M3 - Article

VL - 183

SP - 263

EP - 267

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

SN - 0014-2956

IS - 2

ER -

ID: 4785321