Purification and characterization of the acetone carboxylase of Cupriavidus metallidurans strain CH34

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Abstract

Acetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for the Helicobacteriaceae family, genome analyses revealed that bacterial that possess Acx are associated to the soil such as Cupriavidus metallidurans strain CH34. Acx of CH34 forms a heterohexameric complex α2β2γ2 and can only carboxylate acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.

Details

Original languageEnglish
Pages (from-to)4516-4518
JournalApplied and Environmental Microbiology
Volume78
Issue number12
DOIs
StatePublished - Jun 2012

Keywords

  • Acetone carboxylase, acetone metabolism, Cupriavidus metallidurans CH34

ID: 113000