Quorum-sensing-dependent switch to butanediol fermentation prevents lethal medium acidification in Aeromonas hydrophila AH-1N

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Quorum-sensing-dependent switch to butanediol fermentation prevents lethal medium acidification in Aeromonas hydrophila AH-1N. / Van Houdt, Rob; Aertsen, Abram; Michiels, Chris W.

In: Research in Microbiology, Vol. 158, No. 4, 05.2007, p. 379-385.

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Van Houdt, Rob ; Aertsen, Abram ; Michiels, Chris W. / Quorum-sensing-dependent switch to butanediol fermentation prevents lethal medium acidification in Aeromonas hydrophila AH-1N. In: Research in Microbiology. 2007 ; Vol. 158, No. 4. pp. 379-385.

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@article{ff90904252c04fed83568d630ed325f1,
title = "Quorum-sensing-dependent switch to butanediol fermentation prevents lethal medium acidification in Aeromonas hydrophila AH-1N",
abstract = "Aeromonas hydrophila AH-1N shows biphasic growth in glucose containing broth at 30°C. The first phase is characterized by strong acidification, while the second phase is accompanied by an increase in medium pH. Disruption of AHL production by knockout of ahyI did not affect the first growth phase, but resulted in further acidification which completely blocked the second growth phase and even caused cell death. The block could be relieved by addition of 5 mM (or less) synthetic N-butanoyl-L-homoserine lactone (C4-HSL), the major AHL produced by A. hydrophila AH-1N. Further analysis revealed that, as shown previously for Serratia plymuthica, butanediol fermentation is regulated by AHL-mediated quorum sensing in A. hydrophila AH-1N. These results indicate that A. hydrophila switches to butanediol fermentation to reduce medium acidification under certain conditions and to allow further growth if nutrient resources are not yet exhausted.",
keywords = "Microbiology, Quorum sensing, fermentation, growth",
author = "{Van Houdt}, Rob and Abram Aertsen and Michiels, {Chris W.}",
note = "Score = 10",
year = "2007",
month = may,
doi = "10.1016/j.resmic.2006.11.015",
language = "English",
volume = "158",
pages = "379--385",
journal = "Research in Microbiology",
issn = "0923-2508",
publisher = "Elsevier",
number = "4",

}

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TY - JOUR

T1 - Quorum-sensing-dependent switch to butanediol fermentation prevents lethal medium acidification in Aeromonas hydrophila AH-1N

AU - Van Houdt, Rob

AU - Aertsen, Abram

AU - Michiels, Chris W.

N1 - Score = 10

PY - 2007/5

Y1 - 2007/5

N2 - Aeromonas hydrophila AH-1N shows biphasic growth in glucose containing broth at 30°C. The first phase is characterized by strong acidification, while the second phase is accompanied by an increase in medium pH. Disruption of AHL production by knockout of ahyI did not affect the first growth phase, but resulted in further acidification which completely blocked the second growth phase and even caused cell death. The block could be relieved by addition of 5 mM (or less) synthetic N-butanoyl-L-homoserine lactone (C4-HSL), the major AHL produced by A. hydrophila AH-1N. Further analysis revealed that, as shown previously for Serratia plymuthica, butanediol fermentation is regulated by AHL-mediated quorum sensing in A. hydrophila AH-1N. These results indicate that A. hydrophila switches to butanediol fermentation to reduce medium acidification under certain conditions and to allow further growth if nutrient resources are not yet exhausted.

AB - Aeromonas hydrophila AH-1N shows biphasic growth in glucose containing broth at 30°C. The first phase is characterized by strong acidification, while the second phase is accompanied by an increase in medium pH. Disruption of AHL production by knockout of ahyI did not affect the first growth phase, but resulted in further acidification which completely blocked the second growth phase and even caused cell death. The block could be relieved by addition of 5 mM (or less) synthetic N-butanoyl-L-homoserine lactone (C4-HSL), the major AHL produced by A. hydrophila AH-1N. Further analysis revealed that, as shown previously for Serratia plymuthica, butanediol fermentation is regulated by AHL-mediated quorum sensing in A. hydrophila AH-1N. These results indicate that A. hydrophila switches to butanediol fermentation to reduce medium acidification under certain conditions and to allow further growth if nutrient resources are not yet exhausted.

KW - Microbiology

KW - Quorum sensing

KW - fermentation

KW - growth

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_81105

UR - http://knowledgecentre.sckcen.be/so2/bibref/4288

U2 - 10.1016/j.resmic.2006.11.015

DO - 10.1016/j.resmic.2006.11.015

M3 - Article

VL - 158

SP - 379

EP - 385

JO - Research in Microbiology

JF - Research in Microbiology

SN - 0923-2508

IS - 4

ER -

ID: 166218