Radiation sensitivity of the gastrula-stage embryo: Chromosomeaberrations and mutation induction in lacZ transgenic mice: The rolesof DNA double-strand break repair systems

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Radiation sensitivity of the gastrula-stage embryo: Chromosomeaberrations and mutation induction in lacZ transgenic mice: The rolesof DNA double-strand break repair systems. / Jacquet, Paul; van Buul, Paul; van Duijn-Goedhart, Annemarie; Reynaud, Karine; Buset, Jasmine; Neefs, Mieke; Michaux, Arlette; Monsieurs, Pieter; de Boer, Peter; Baatout, Sarah.

In: Mutation Research, Vol. 792, No. 10, 10.2015, p. 26-34.

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@article{86dc498d104b4acbaa3db9d2fed8b22e,
title = "Radiation sensitivity of the gastrula-stage embryo: Chromosomeaberrations and mutation induction in lacZ transgenic mice: The rolesof DNA double-strand break repair systems",
abstract = "At the gastrula phase of development, just after the onset of implantation, the embryo proper is characterized by extremely rapid cell proliferation. The importance of DNA repair is illustrated by embryonic lethality at this stage after ablation of the genes involved. Insight into mutation induction is called for by the fact that women often do not realize they are pregnant, shortly after implantation, a circumstance which may have important consequences when women are subjected to medical imaging using ionizing radiation. We screened gastrula embryos for DNA synthesis, nuclear morphology, growth, and chromosome aberrations (CA) shortly after irradiation with doses up to 2.5 Gy. In order to obtain an insight into the importance of DNA repair for CA induction, we included mutants for the non-homologous end joining (NHEJ) and homologous recombination repair (HRR) pathways, as well as Parp1-/- and p53+/−embryos.",
keywords = "Embryo, Gastrula, Irradiation, DNA repair genes, lacZ shuttle vector",
author = "Paul Jacquet and {van Buul}, Paul and {van Duijn-Goedhart}, Annemarie and Karine Reynaud and Jasmine Buset and Mieke Neefs and Arlette Michaux and Pieter Monsieurs and {de Boer}, Peter and Sarah Baatout",
note = "Score = 10",
year = "2015",
month = oct,
doi = "10.1016/j.mrgentox.2015.09.001",
language = "English",
volume = "792",
pages = "26--34",
journal = "Mutation Research",
issn = "1383-5718",
publisher = "Elsevier",
number = "10",

}

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TY - JOUR

T1 - Radiation sensitivity of the gastrula-stage embryo: Chromosomeaberrations and mutation induction in lacZ transgenic mice: The rolesof DNA double-strand break repair systems

AU - Jacquet, Paul

AU - van Buul, Paul

AU - van Duijn-Goedhart, Annemarie

AU - Reynaud, Karine

AU - Buset, Jasmine

AU - Neefs, Mieke

AU - Michaux, Arlette

AU - Monsieurs, Pieter

AU - de Boer, Peter

AU - Baatout, Sarah

N1 - Score = 10

PY - 2015/10

Y1 - 2015/10

N2 - At the gastrula phase of development, just after the onset of implantation, the embryo proper is characterized by extremely rapid cell proliferation. The importance of DNA repair is illustrated by embryonic lethality at this stage after ablation of the genes involved. Insight into mutation induction is called for by the fact that women often do not realize they are pregnant, shortly after implantation, a circumstance which may have important consequences when women are subjected to medical imaging using ionizing radiation. We screened gastrula embryos for DNA synthesis, nuclear morphology, growth, and chromosome aberrations (CA) shortly after irradiation with doses up to 2.5 Gy. In order to obtain an insight into the importance of DNA repair for CA induction, we included mutants for the non-homologous end joining (NHEJ) and homologous recombination repair (HRR) pathways, as well as Parp1-/- and p53+/−embryos.

AB - At the gastrula phase of development, just after the onset of implantation, the embryo proper is characterized by extremely rapid cell proliferation. The importance of DNA repair is illustrated by embryonic lethality at this stage after ablation of the genes involved. Insight into mutation induction is called for by the fact that women often do not realize they are pregnant, shortly after implantation, a circumstance which may have important consequences when women are subjected to medical imaging using ionizing radiation. We screened gastrula embryos for DNA synthesis, nuclear morphology, growth, and chromosome aberrations (CA) shortly after irradiation with doses up to 2.5 Gy. In order to obtain an insight into the importance of DNA repair for CA induction, we included mutants for the non-homologous end joining (NHEJ) and homologous recombination repair (HRR) pathways, as well as Parp1-/- and p53+/−embryos.

KW - Embryo

KW - Gastrula

KW - Irradiation

KW - DNA repair genes

KW - lacZ shuttle vector

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_140801

UR - http://knowledgecentre.sckcen.be/so2/bibref/13047

U2 - 10.1016/j.mrgentox.2015.09.001

DO - 10.1016/j.mrgentox.2015.09.001

M3 - Article

VL - 792

SP - 26

EP - 34

JO - Mutation Research

JF - Mutation Research

SN - 1383-5718

IS - 10

ER -

ID: 85632