Radiation-induced double strand breaks and subsequent apoptotic DNA fragmentation in human peripheral blood mononuclear cells

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Radiation-induced double strand breaks and subsequent apoptotic DNA fragmentation in human peripheral blood mononuclear cells. / Ghardi, Myriam; Moreels, Marjan; Chatelain, Bernard; Chatelain, Christian; Baatout, Sarah.

In: International Journal of Molecular Medicine, Vol. 29, No. 5, 01.01.2012, p. 769-780.

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Ghardi, Myriam ; Moreels, Marjan ; Chatelain, Bernard ; Chatelain, Christian ; Baatout, Sarah. / Radiation-induced double strand breaks and subsequent apoptotic DNA fragmentation in human peripheral blood mononuclear cells. In: International Journal of Molecular Medicine. 2012 ; Vol. 29, No. 5. pp. 769-780.

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@article{b29908ce69a248d78f7e8bf7b1bb1e31,
title = "Radiation-induced double strand breaks and subsequent apoptotic DNA fragmentation in human peripheral blood mononuclear cells",
abstract = "In case of accidental radiation exposure or a nuclear incident, physical dosimetry is not always complete. Therefore, it is important to develop tools that allow dose estimates and determination that are based on biological markers of radiation exposure. Exposure to ionizing radiation triggers a large-scale activation of specific DNA signaling and repair mechanisms. This includes the phosphorylation of γH2AX in the vicinity of a double-strand break (DSB). A DNA DSB is a cytotoxic form of DNA damage, and if not correctly repaired can initiate genomic instability, chromosome aberrations, mutations or apoptosis. Measurements of DNA DSBs and their subsequent repair after in vitro irradiation has been suggested to be of potential use to monitor cellular responses. The bone marrow and the blood are known to be the most radiosensitive tissues of the human body and can therefore be of particular importance to find radiation-induced biological markers. In the present study, changes in H2AX phosphorylation and apoptosis of irradiated human peripheral blood mononuclear cells (PBMCs) were analyzed.",
keywords = "DNA damage, DNA repair, DSB repair kinetics, apoptosis, γH2AX, biodosimetry, ionizing radiation",
author = "Myriam Ghardi and Marjan Moreels and Bernard Chatelain and Christian Chatelain and Sarah Baatout",
note = "Score = 10",
year = "2012",
month = "1",
day = "1",
doi = "10.3892/ijmm.2012.907",
language = "English",
volume = "29",
pages = "769--780",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "5",

}

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TY - JOUR

T1 - Radiation-induced double strand breaks and subsequent apoptotic DNA fragmentation in human peripheral blood mononuclear cells

AU - Ghardi, Myriam

AU - Moreels, Marjan

AU - Chatelain, Bernard

AU - Chatelain, Christian

AU - Baatout, Sarah

N1 - Score = 10

PY - 2012/1/1

Y1 - 2012/1/1

N2 - In case of accidental radiation exposure or a nuclear incident, physical dosimetry is not always complete. Therefore, it is important to develop tools that allow dose estimates and determination that are based on biological markers of radiation exposure. Exposure to ionizing radiation triggers a large-scale activation of specific DNA signaling and repair mechanisms. This includes the phosphorylation of γH2AX in the vicinity of a double-strand break (DSB). A DNA DSB is a cytotoxic form of DNA damage, and if not correctly repaired can initiate genomic instability, chromosome aberrations, mutations or apoptosis. Measurements of DNA DSBs and their subsequent repair after in vitro irradiation has been suggested to be of potential use to monitor cellular responses. The bone marrow and the blood are known to be the most radiosensitive tissues of the human body and can therefore be of particular importance to find radiation-induced biological markers. In the present study, changes in H2AX phosphorylation and apoptosis of irradiated human peripheral blood mononuclear cells (PBMCs) were analyzed.

AB - In case of accidental radiation exposure or a nuclear incident, physical dosimetry is not always complete. Therefore, it is important to develop tools that allow dose estimates and determination that are based on biological markers of radiation exposure. Exposure to ionizing radiation triggers a large-scale activation of specific DNA signaling and repair mechanisms. This includes the phosphorylation of γH2AX in the vicinity of a double-strand break (DSB). A DNA DSB is a cytotoxic form of DNA damage, and if not correctly repaired can initiate genomic instability, chromosome aberrations, mutations or apoptosis. Measurements of DNA DSBs and their subsequent repair after in vitro irradiation has been suggested to be of potential use to monitor cellular responses. The bone marrow and the blood are known to be the most radiosensitive tissues of the human body and can therefore be of particular importance to find radiation-induced biological markers. In the present study, changes in H2AX phosphorylation and apoptosis of irradiated human peripheral blood mononuclear cells (PBMCs) were analyzed.

KW - DNA damage

KW - DNA repair

KW - DSB repair kinetics

KW - apoptosis

KW - γH2AX

KW - biodosimetry

KW - ionizing radiation

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_122808

UR - http://knowledgecentre.sckcen.be/so2/bibref/9335

U2 - 10.3892/ijmm.2012.907

DO - 10.3892/ijmm.2012.907

M3 - Article

VL - 29

SP - 769

EP - 780

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 5

ER -

ID: 110343