Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity

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Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity. / De Gelder, Joke; Vandenabeele, Peter; De Boever, Patrick; Mergeay, Max; Moens, Luc; De Vos, Paul.

In: Microgravity - Science and Technology, 25.03.2008, p. 1-7.

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De Gelder, Joke ; Vandenabeele, Peter ; De Boever, Patrick ; Mergeay, Max ; Moens, Luc ; De Vos, Paul. / Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity. In: Microgravity - Science and Technology. 2008 ; pp. 1-7.

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@article{e27298ae79544dc680cc0cd720503814,
title = "Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity",
abstract = "In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a rotating wall vessel to simulate microgravity (SMG) and in a control setup. The differences in Raman spectra recorded from both setups after 24 h of culturing were small. The most prominent features in a difference spectrum, calculated between the mean spectra from the microgravity and the control setup separately, could be assigned to the presence of poly-β-hydroxybutyrate (PHB). SMG seems to yield a higher PHB production after 24 h of culturing. Additional processing of the spectra suggested that SMG induced also other changes in the carbon-metabolism. After 48 h, similar results were found for the carbon metabolism, while PHB concentrations were reduced in SMG compared to the control. However, these differences could also be caused by interfering effects that may occur in the bioreactors after a prolonged incubation time.",
keywords = "Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity",
author = "{De Gelder}, Joke and Peter Vandenabeele and {De Boever}, Patrick and Max Mergeay and Luc Moens and {De Vos}, Paul",
note = "Score = 10",
year = "2008",
month = "3",
day = "25",
doi = "10.1007/s12217-008-9037-0",
language = "English",
pages = "1--7",
journal = "Microgravity - Science and Technology",
issn = "0938-0108",
publisher = "Springer",

}

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TY - JOUR

T1 - Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity

AU - De Gelder, Joke

AU - Vandenabeele, Peter

AU - De Boever, Patrick

AU - Mergeay, Max

AU - Moens, Luc

AU - De Vos, Paul

N1 - Score = 10

PY - 2008/3/25

Y1 - 2008/3/25

N2 - In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a rotating wall vessel to simulate microgravity (SMG) and in a control setup. The differences in Raman spectra recorded from both setups after 24 h of culturing were small. The most prominent features in a difference spectrum, calculated between the mean spectra from the microgravity and the control setup separately, could be assigned to the presence of poly-β-hydroxybutyrate (PHB). SMG seems to yield a higher PHB production after 24 h of culturing. Additional processing of the spectra suggested that SMG induced also other changes in the carbon-metabolism. After 48 h, similar results were found for the carbon metabolism, while PHB concentrations were reduced in SMG compared to the control. However, these differences could also be caused by interfering effects that may occur in the bioreactors after a prolonged incubation time.

AB - In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a rotating wall vessel to simulate microgravity (SMG) and in a control setup. The differences in Raman spectra recorded from both setups after 24 h of culturing were small. The most prominent features in a difference spectrum, calculated between the mean spectra from the microgravity and the control setup separately, could be assigned to the presence of poly-β-hydroxybutyrate (PHB). SMG seems to yield a higher PHB production after 24 h of culturing. Additional processing of the spectra suggested that SMG induced also other changes in the carbon-metabolism. After 48 h, similar results were found for the carbon metabolism, while PHB concentrations were reduced in SMG compared to the control. However, these differences could also be caused by interfering effects that may occur in the bioreactors after a prolonged incubation time.

KW - Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_94936

UR - http://knowledgecentre.sckcen.be/so2/bibref/5608

U2 - 10.1007/s12217-008-9037-0

DO - 10.1007/s12217-008-9037-0

M3 - Article

SP - 1

EP - 7

JO - Microgravity - Science and Technology

JF - Microgravity - Science and Technology

SN - 0938-0108

ER -

ID: 137557