Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-β

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Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-β. / Dieriks, Birger; De Vos, Winnok; Baatout, Sarah; Van Oostveldt, Patrick; Jacquet, Paul (Peer reviewer).

In: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 715, No. 1-2, 08.07.2011, p. 19-24.

Research output: Contribution to journalArticlepeer-review

Harvard

Dieriks, B, De Vos, W, Baatout, S, Van Oostveldt, P & Jacquet, P 2011, 'Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-β', Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, vol. 715, no. 1-2, pp. 19-24. https://doi.org/10.1016/j.mrfmmm.2011.07.002

APA

Dieriks, B., De Vos, W., Baatout, S., Van Oostveldt, P., & Jacquet, P. (2011). Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-β. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 715(1-2), 19-24. https://doi.org/10.1016/j.mrfmmm.2011.07.002

Vancouver

Dieriks B, De Vos W, Baatout S, Van Oostveldt P, Jacquet P. Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-β. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2011 Jul 8;715(1-2):19-24. https://doi.org/10.1016/j.mrfmmm.2011.07.002

Author

Dieriks, Birger ; De Vos, Winnok ; Baatout, Sarah ; Van Oostveldt, Patrick ; Jacquet, Paul. / Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-β. In: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2011 ; Vol. 715, No. 1-2. pp. 19-24.

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@article{203c527351a14f2e802546a1e4f4c3a4,
title = "Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-β",
abstract = "Exposing cells to a low dose can protect them against a subsequent higher exposure. This phenomenon is known as adaptive response and is frequently observed in a variety of cells. Even though similarities are suspected with other non-targeted effects, such as bystander effects, the exact mechanism behind adaptive response is not fully clarified. In this study human primary fibroblasts were tested for their response to ionizing radiation (IR) after administrating a low priming dose (0.1–0.5 Gy). Both the abundance of γH2AX as a marker for double-stranded breaks and the levels of cytokines, secreted in the medium, were monitored in time. Upon challenge, IR-primed cells showed modified γH2AX spot size distributions and altered repair kinetics, consistent with an adaptive response. In addition, 24 h after priming with IR, four cytokines were significantly upregulated in the medium – GM-CSF (1.33×); IL6 (4.24×); IL8 (1.33×); TGF-β (1.46×). In order to mimick the protective effect of IR priming, we primed the cells with either IL6 or TGF-β. This did not elicit an altered γH2AX response as observed in IR-primed cells, indicating that the adaptive response in these primary fibroblasts is regulated in an IL-6 and TGF-β independent manner.",
keywords = "Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-β",
author = "Birger Dieriks and {De Vos}, Winnok and Sarah Baatout and {Van Oostveldt}, Patrick and Paul Jacquet",
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year = "2011",
month = jul,
day = "8",
doi = "10.1016/j.mrfmmm.2011.07.002",
language = "English",
volume = "715",
pages = "19--24",
journal = "Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis",
issn = "0027-5107",
publisher = "Elsevier",
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}

RIS - Download

TY - JOUR

T1 - Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-β

AU - Dieriks, Birger

AU - De Vos, Winnok

AU - Baatout, Sarah

AU - Van Oostveldt, Patrick

A2 - Jacquet, Paul

N1 - Score = 10

PY - 2011/7/8

Y1 - 2011/7/8

N2 - Exposing cells to a low dose can protect them against a subsequent higher exposure. This phenomenon is known as adaptive response and is frequently observed in a variety of cells. Even though similarities are suspected with other non-targeted effects, such as bystander effects, the exact mechanism behind adaptive response is not fully clarified. In this study human primary fibroblasts were tested for their response to ionizing radiation (IR) after administrating a low priming dose (0.1–0.5 Gy). Both the abundance of γH2AX as a marker for double-stranded breaks and the levels of cytokines, secreted in the medium, were monitored in time. Upon challenge, IR-primed cells showed modified γH2AX spot size distributions and altered repair kinetics, consistent with an adaptive response. In addition, 24 h after priming with IR, four cytokines were significantly upregulated in the medium – GM-CSF (1.33×); IL6 (4.24×); IL8 (1.33×); TGF-β (1.46×). In order to mimick the protective effect of IR priming, we primed the cells with either IL6 or TGF-β. This did not elicit an altered γH2AX response as observed in IR-primed cells, indicating that the adaptive response in these primary fibroblasts is regulated in an IL-6 and TGF-β independent manner.

AB - Exposing cells to a low dose can protect them against a subsequent higher exposure. This phenomenon is known as adaptive response and is frequently observed in a variety of cells. Even though similarities are suspected with other non-targeted effects, such as bystander effects, the exact mechanism behind adaptive response is not fully clarified. In this study human primary fibroblasts were tested for their response to ionizing radiation (IR) after administrating a low priming dose (0.1–0.5 Gy). Both the abundance of γH2AX as a marker for double-stranded breaks and the levels of cytokines, secreted in the medium, were monitored in time. Upon challenge, IR-primed cells showed modified γH2AX spot size distributions and altered repair kinetics, consistent with an adaptive response. In addition, 24 h after priming with IR, four cytokines were significantly upregulated in the medium – GM-CSF (1.33×); IL6 (4.24×); IL8 (1.33×); TGF-β (1.46×). In order to mimick the protective effect of IR priming, we primed the cells with either IL6 or TGF-β. This did not elicit an altered γH2AX response as observed in IR-primed cells, indicating that the adaptive response in these primary fibroblasts is regulated in an IL-6 and TGF-β independent manner.

KW - Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-β

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_116440

UR - http://knowledgecentre.sckcen.be/so2/bibref/8393

U2 - 10.1016/j.mrfmmm.2011.07.002

DO - 10.1016/j.mrfmmm.2011.07.002

M3 - Article

VL - 715

SP - 19

EP - 24

JO - Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis

SN - 0027-5107

IS - 1-2

ER -

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