Rock geochemistry induces stress and starvation responses in the bacterial proteome

Research output: Contribution to journalArticle

Authors

  • Natalie Leys
  • Casey C. Bryce
  • Thierry Le Bihan
  • Sarah F. Martin
  • Timothy Bush
  • Brian Spears
  • Alanna Moore
  • Bo Byloos
  • Charles S. Cockell

Institutes & Expert groups

  • University of Edinburgh - UK Centre for Astrobiology
  • University of Edinburgh - Centre for Synthetic and Systems Biology
  • University of Edinburgh
  • NERC-CEH - Natural Environment Research Council : Centre for Ecology and Hydrology - UK

Documents & links

DOI

Abstract

Interactions between microorganisms and rocks play an important role in Earth system processes. However, little is known about the molecular capabilities microorganisms require to live in rocky environments. Using a quantitative label‐free proteomics approach, we show that a model bacterium (Cupriavidus metallidurans CH34) can use volcanic rock to satisfy some elemental requirements, resulting in increased rates of cell division in both magnesium‐ and iron‐limited media. However, the rocks also introduced multiple new stresses via chemical changes associated with pH, elemental leaching and surface adsorption of nutrients that were reflected in the proteome. For example, the loss of bioavailable phosphorus was observed and resulted in the upregulation of diverse phosphate limitation proteins, which facilitate increase phosphate uptake and scavenging within the cell. Our results revealed that despite the provision of essential elements, rock chemistry drives complex metabolic reorganization within rock‐dwelling organisms, requiring tight regulation of cellular processes at the protein level. This study advances our ability to identify key microbial responses that enable life to persist in rock environments.

Details

Original languageEnglish
Pages (from-to)1110-1121
Number of pages12
JournalEnvironmental Microbiology
Volume18
Issue number4
DOIs
Publication statusPublished - 1 Apr 2016

Keywords

  • Dissolution, Diversity, Abundance, Minerals, Life

ID: 5655448