Simulated microgravity decreases apoptosis in fetal fibroblasts

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Simulated microgravity decreases apoptosis in fetal fibroblasts. / Beck, Michaël; Tabury, Kevin; Moreels, Marjan; Jacquet, Paul; Van Oostveldt, Patrick; De Vos, Winnok H.; Baatout, Sarah.

In: International Journal of Molecular Medicine, Vol. 30, No. 2, 20.02.2012, p. 309-313.

Research output: Contribution to journalArticlepeer-review

Harvard

Beck, M, Tabury, K, Moreels, M, Jacquet, P, Van Oostveldt, P, De Vos, WH & Baatout, S 2012, 'Simulated microgravity decreases apoptosis in fetal fibroblasts', International Journal of Molecular Medicine, vol. 30, no. 2, pp. 309-313. https://doi.org/10.3892/ijmm.2012.1001

APA

Vancouver

Beck M, Tabury K, Moreels M, Jacquet P, Van Oostveldt P, De Vos WH et al. Simulated microgravity decreases apoptosis in fetal fibroblasts. International Journal of Molecular Medicine. 2012 Feb 20;30(2):309-313. https://doi.org/10.3892/ijmm.2012.1001

Author

Beck, Michaël ; Tabury, Kevin ; Moreels, Marjan ; Jacquet, Paul ; Van Oostveldt, Patrick ; De Vos, Winnok H. ; Baatout, Sarah. / Simulated microgravity decreases apoptosis in fetal fibroblasts. In: International Journal of Molecular Medicine. 2012 ; Vol. 30, No. 2. pp. 309-313.

Bibtex - Download

@article{ddb8df2975a8411298e7d0c96eba79e0,
title = "Simulated microgravity decreases apoptosis in fetal fibroblasts",
abstract = "Space travel is a major challenge for human beings. Especially, the mechanisms through which space conditions might alter animal development have been questioned for a long time. The two major physical stress factors that are of relevance in this context are space radiation and weightlessness. While it has been extensively shown that high doses of ionizing radiation induce deleterious effects on embryonic development, so far, little is known about the potential harmful effects of radiation in combination with microgravity on the developing organism. In the present study, we investigated the effects of simulated microgravity on irradiated STO mouse fetal fibroblast cells using a random positioning machine (RPM). Radiation-induced cell cycle changes were not affected when cells were subjected to simulated microgravity for 24 h. Moreover, no morphological differences were observed in irradiated samples exposed to simulated microgravity compared to cells that were exclusively irradiated. However, microgravity simulation significantly decreased the level of apoptosis at all doses as measured by caspase-3 activity and it prevented cells from undergoing radiation-induced size increase up to 1 Gy.",
keywords = "X-rays, simulated microgravity, fetal mouse fibroblasts, DNA damage, cell cycle arrest, apoptosis",
author = "Micha{\"e}l Beck and Kevin Tabury and Marjan Moreels and Paul Jacquet and {Van Oostveldt}, Patrick and {De Vos}, {Winnok H.} and Sarah Baatout",
note = "Score = 10",
year = "2012",
month = feb,
day = "20",
doi = "10.3892/ijmm.2012.1001",
language = "English",
volume = "30",
pages = "309--313",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "2",

}

RIS - Download

TY - JOUR

T1 - Simulated microgravity decreases apoptosis in fetal fibroblasts

AU - Beck, Michaël

AU - Tabury, Kevin

AU - Moreels, Marjan

AU - Jacquet, Paul

AU - Van Oostveldt, Patrick

AU - De Vos, Winnok H.

AU - Baatout, Sarah

N1 - Score = 10

PY - 2012/2/20

Y1 - 2012/2/20

N2 - Space travel is a major challenge for human beings. Especially, the mechanisms through which space conditions might alter animal development have been questioned for a long time. The two major physical stress factors that are of relevance in this context are space radiation and weightlessness. While it has been extensively shown that high doses of ionizing radiation induce deleterious effects on embryonic development, so far, little is known about the potential harmful effects of radiation in combination with microgravity on the developing organism. In the present study, we investigated the effects of simulated microgravity on irradiated STO mouse fetal fibroblast cells using a random positioning machine (RPM). Radiation-induced cell cycle changes were not affected when cells were subjected to simulated microgravity for 24 h. Moreover, no morphological differences were observed in irradiated samples exposed to simulated microgravity compared to cells that were exclusively irradiated. However, microgravity simulation significantly decreased the level of apoptosis at all doses as measured by caspase-3 activity and it prevented cells from undergoing radiation-induced size increase up to 1 Gy.

AB - Space travel is a major challenge for human beings. Especially, the mechanisms through which space conditions might alter animal development have been questioned for a long time. The two major physical stress factors that are of relevance in this context are space radiation and weightlessness. While it has been extensively shown that high doses of ionizing radiation induce deleterious effects on embryonic development, so far, little is known about the potential harmful effects of radiation in combination with microgravity on the developing organism. In the present study, we investigated the effects of simulated microgravity on irradiated STO mouse fetal fibroblast cells using a random positioning machine (RPM). Radiation-induced cell cycle changes were not affected when cells were subjected to simulated microgravity for 24 h. Moreover, no morphological differences were observed in irradiated samples exposed to simulated microgravity compared to cells that were exclusively irradiated. However, microgravity simulation significantly decreased the level of apoptosis at all doses as measured by caspase-3 activity and it prevented cells from undergoing radiation-induced size increase up to 1 Gy.

KW - X-rays

KW - simulated microgravity

KW - fetal mouse fibroblasts

KW - DNA damage

KW - cell cycle arrest

KW - apoptosis

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_122675

UR - http://knowledgecentre.sckcen.be/so2/bibref/9325

U2 - 10.3892/ijmm.2012.1001

DO - 10.3892/ijmm.2012.1001

M3 - Article

VL - 30

SP - 309

EP - 313

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 2

ER -

ID: 145199