Stress-induced mutagenesis in Cupriavidus metallidurans strains

Research output: ThesisMaster's thesis

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Stress-induced mutagenesis in Cupriavidus metallidurans strains. / Poppe, Michaël; Vandecraen, Joachim; Van Houdt, Rob; Mijnendonckx, Kristel (Peer reviewer); Moors, Hugo (Peer reviewer).

Leuven, Belgium : KUL - Katholieke Universiteit Leuven, 2014. 96 p.

Research output: ThesisMaster's thesis

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Poppe M, Vandecraen J, Van Houdt R, Mijnendonckx K, Moors H. Stress-induced mutagenesis in Cupriavidus metallidurans strains. Leuven, Belgium: KUL - Katholieke Universiteit Leuven, 2014. 96 p.

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@phdthesis{08ddec69fb664d16861791a480e39cad,
title = "Stress-induced mutagenesis in Cupriavidus metallidurans strains",
abstract = "This thesis had a two-sided purpose: The first was to determine which processes & molecules are responsible for the TIMM-phenotype. TIMM stands for temperature-induced mortality and mutagenesis and was first seen when incubating Cupriavidus metallidurans CH34 on rich medium at 37 °C. Narrowing down the components that could induce TIMM was a first objective. Several amino acids were able to induce TIMM per tested strain, but different patterns arose between the tested strains. Inductors of TIMM seem independent of each other, one being the metabolization of the amino acids and the other being related to osmotic potential. Mitigation of TIMM was induced by adding neutral osmolytes, while the use of reactive oxygen scavengers scavengers had little to no effect. A time-lapse microscope experiment was performed to see if changes could be detected while TIMM was induced. Characteristics like cell-elongation, reproduction halting and cell-lysis were induced. The second purpose was to increase zinc-resistance in the C. metallidurans CH34 and AE104 strains. This was done by directed evolution, or growing the bacterium in medium with zinc concentrations above their respective minimal inhibitory concentration (MIC). Both strains were successfully evolved to resistances of 0,5 mM for AE104 and 26 mM for CH34 respectively.",
keywords = "Cupriavidus metallidurans, TIMM, Zinc, Mutagenesis",
author = "Micha{\"e}l Poppe and Joachim Vandecraen and {Van Houdt}, Rob and Kristel Mijnendonckx and Hugo Moors",
note = "Score = 2",
year = "2014",
month = "6",
language = "English",
publisher = "KUL - Katholieke Universiteit Leuven",
school = "KUL - Katholieke Universiteit Leuven",

}

RIS - Download

TY - THES

T1 - Stress-induced mutagenesis in Cupriavidus metallidurans strains

AU - Poppe, Michaël

AU - Vandecraen, Joachim

AU - Van Houdt, Rob

A2 - Mijnendonckx, Kristel

A2 - Moors, Hugo

N1 - Score = 2

PY - 2014/6

Y1 - 2014/6

N2 - This thesis had a two-sided purpose: The first was to determine which processes & molecules are responsible for the TIMM-phenotype. TIMM stands for temperature-induced mortality and mutagenesis and was first seen when incubating Cupriavidus metallidurans CH34 on rich medium at 37 °C. Narrowing down the components that could induce TIMM was a first objective. Several amino acids were able to induce TIMM per tested strain, but different patterns arose between the tested strains. Inductors of TIMM seem independent of each other, one being the metabolization of the amino acids and the other being related to osmotic potential. Mitigation of TIMM was induced by adding neutral osmolytes, while the use of reactive oxygen scavengers scavengers had little to no effect. A time-lapse microscope experiment was performed to see if changes could be detected while TIMM was induced. Characteristics like cell-elongation, reproduction halting and cell-lysis were induced. The second purpose was to increase zinc-resistance in the C. metallidurans CH34 and AE104 strains. This was done by directed evolution, or growing the bacterium in medium with zinc concentrations above their respective minimal inhibitory concentration (MIC). Both strains were successfully evolved to resistances of 0,5 mM for AE104 and 26 mM for CH34 respectively.

AB - This thesis had a two-sided purpose: The first was to determine which processes & molecules are responsible for the TIMM-phenotype. TIMM stands for temperature-induced mortality and mutagenesis and was first seen when incubating Cupriavidus metallidurans CH34 on rich medium at 37 °C. Narrowing down the components that could induce TIMM was a first objective. Several amino acids were able to induce TIMM per tested strain, but different patterns arose between the tested strains. Inductors of TIMM seem independent of each other, one being the metabolization of the amino acids and the other being related to osmotic potential. Mitigation of TIMM was induced by adding neutral osmolytes, while the use of reactive oxygen scavengers scavengers had little to no effect. A time-lapse microscope experiment was performed to see if changes could be detected while TIMM was induced. Characteristics like cell-elongation, reproduction halting and cell-lysis were induced. The second purpose was to increase zinc-resistance in the C. metallidurans CH34 and AE104 strains. This was done by directed evolution, or growing the bacterium in medium with zinc concentrations above their respective minimal inhibitory concentration (MIC). Both strains were successfully evolved to resistances of 0,5 mM for AE104 and 26 mM for CH34 respectively.

KW - Cupriavidus metallidurans

KW - TIMM

KW - Zinc

KW - Mutagenesis

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_135650

M3 - Master's thesis

PB - KUL - Katholieke Universiteit Leuven

CY - Leuven, Belgium

ER -

ID: 164196