Studies on the adaptive response in mouse female germ cells X-irradiated in vitro at two different stages of maturation

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Studies on the adaptive response in mouse female germ cells X-irradiated in vitro at two different stages of maturation. / Jacquet, Paul; Buset, Jasmine; Neefs, Mieke; Vankerkom, Jean; Baatout, Sarah (Peer reviewer).

In: In Vivo, Vol. 22, No. 2, 01.2008, p. 179-186.

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@article{28e0302b8b624f949a81ba3a15ce8820,
title = "Studies on the adaptive response in mouse female germ cells X-irradiated in vitro at two different stages of maturation",
abstract = "Radioadaptation is a phenomenon whereby cells exposed to a low dose (conditioning dose) of ionizing radiation are more resistant to a dose in the order of a few Gy (challenge dose) delivered some time thereafter. It is generally postulated that radioadaptation could result from the activation of damage repair and/or antioxidant defence systems. We used a recently developed in vitro system to investigate the possibility of a cytogenetic adaptive response in female germ cells X-irradiated at 2 well characterized stages of maturation. Mouse ovarian follicles were cultured from an early preantral stage (1-2 layers of follicle cells) up to ovulation. Hormonal stimulation of ovulation was realized by addition of hCG and EGF to the culture medium on day 12. The follicles were X-irradiated with either 2 or 4 Gy preceded or not by 50 mGy (“conditioning dose”, 5 h earlier), on days 0 or 12 of the culture. Ovulated oocytes were collected on day 13, fixed and analyzed for the presence of chromosome aberrations. The results suggested that pre-irradiation of mouse pre-ovulatory oocytes with a low conditioning dose of X-rays could confer them some protection against radiation-induction of chromosome damage by a subsequent challenge dose of a few Gy.",
keywords = "adaptive response, oocytes, follicle culture, ionizing radiation, chromosome aberrations",
author = "Paul Jacquet and Jasmine Buset and Mieke Neefs and Jean Vankerkom and Sarah Baatout",
note = "Score = 10",
year = "2008",
month = jan,
language = "English",
volume = "22",
pages = "179--186",
journal = "In Vivo",
issn = "0258-851X",
publisher = "IIAR - International Institute of Anticancer Research",
number = "2",

}

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TY - JOUR

T1 - Studies on the adaptive response in mouse female germ cells X-irradiated in vitro at two different stages of maturation

AU - Jacquet, Paul

AU - Buset, Jasmine

AU - Neefs, Mieke

AU - Vankerkom, Jean

A2 - Baatout, Sarah

N1 - Score = 10

PY - 2008/1

Y1 - 2008/1

N2 - Radioadaptation is a phenomenon whereby cells exposed to a low dose (conditioning dose) of ionizing radiation are more resistant to a dose in the order of a few Gy (challenge dose) delivered some time thereafter. It is generally postulated that radioadaptation could result from the activation of damage repair and/or antioxidant defence systems. We used a recently developed in vitro system to investigate the possibility of a cytogenetic adaptive response in female germ cells X-irradiated at 2 well characterized stages of maturation. Mouse ovarian follicles were cultured from an early preantral stage (1-2 layers of follicle cells) up to ovulation. Hormonal stimulation of ovulation was realized by addition of hCG and EGF to the culture medium on day 12. The follicles were X-irradiated with either 2 or 4 Gy preceded or not by 50 mGy (“conditioning dose”, 5 h earlier), on days 0 or 12 of the culture. Ovulated oocytes were collected on day 13, fixed and analyzed for the presence of chromosome aberrations. The results suggested that pre-irradiation of mouse pre-ovulatory oocytes with a low conditioning dose of X-rays could confer them some protection against radiation-induction of chromosome damage by a subsequent challenge dose of a few Gy.

AB - Radioadaptation is a phenomenon whereby cells exposed to a low dose (conditioning dose) of ionizing radiation are more resistant to a dose in the order of a few Gy (challenge dose) delivered some time thereafter. It is generally postulated that radioadaptation could result from the activation of damage repair and/or antioxidant defence systems. We used a recently developed in vitro system to investigate the possibility of a cytogenetic adaptive response in female germ cells X-irradiated at 2 well characterized stages of maturation. Mouse ovarian follicles were cultured from an early preantral stage (1-2 layers of follicle cells) up to ovulation. Hormonal stimulation of ovulation was realized by addition of hCG and EGF to the culture medium on day 12. The follicles were X-irradiated with either 2 or 4 Gy preceded or not by 50 mGy (“conditioning dose”, 5 h earlier), on days 0 or 12 of the culture. Ovulated oocytes were collected on day 13, fixed and analyzed for the presence of chromosome aberrations. The results suggested that pre-irradiation of mouse pre-ovulatory oocytes with a low conditioning dose of X-rays could confer them some protection against radiation-induction of chromosome damage by a subsequent challenge dose of a few Gy.

KW - adaptive response

KW - oocytes

KW - follicle culture

KW - ionizing radiation

KW - chromosome aberrations

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_92270

UR - http://knowledgecentre.sckcen.be/so2/bibref/5250

M3 - Article

VL - 22

SP - 179

EP - 186

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 2

ER -

ID: 209565