Studies on the adaptive response in mouse female germ cells X-irradiated in vitro at two different stages of maturation
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Studies on the adaptive response in mouse female germ cells X-irradiated in vitro at two different stages of maturation. / Jacquet, Paul; Buset, Jasmine; Neefs, Mieke; Vankerkom, Jean; Baatout, Sarah (Peer reviewer).
In: In Vivo, Vol. 22, No. 2, 01.2008, p. 179-186.Research output: Contribution to journal › Article › peer-review
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T1 - Studies on the adaptive response in mouse female germ cells X-irradiated in vitro at two different stages of maturation
AU - Jacquet, Paul
AU - Buset, Jasmine
AU - Neefs, Mieke
AU - Vankerkom, Jean
A2 - Baatout, Sarah
N1 - Score = 10
PY - 2008/1
Y1 - 2008/1
N2 - Radioadaptation is a phenomenon whereby cells exposed to a low dose (conditioning dose) of ionizing radiation are more resistant to a dose in the order of a few Gy (challenge dose) delivered some time thereafter. It is generally postulated that radioadaptation could result from the activation of damage repair and/or antioxidant defence systems. We used a recently developed in vitro system to investigate the possibility of a cytogenetic adaptive response in female germ cells X-irradiated at 2 well characterized stages of maturation. Mouse ovarian follicles were cultured from an early preantral stage (1-2 layers of follicle cells) up to ovulation. Hormonal stimulation of ovulation was realized by addition of hCG and EGF to the culture medium on day 12. The follicles were X-irradiated with either 2 or 4 Gy preceded or not by 50 mGy (“conditioning dose”, 5 h earlier), on days 0 or 12 of the culture. Ovulated oocytes were collected on day 13, fixed and analyzed for the presence of chromosome aberrations. The results suggested that pre-irradiation of mouse pre-ovulatory oocytes with a low conditioning dose of X-rays could confer them some protection against radiation-induction of chromosome damage by a subsequent challenge dose of a few Gy.
AB - Radioadaptation is a phenomenon whereby cells exposed to a low dose (conditioning dose) of ionizing radiation are more resistant to a dose in the order of a few Gy (challenge dose) delivered some time thereafter. It is generally postulated that radioadaptation could result from the activation of damage repair and/or antioxidant defence systems. We used a recently developed in vitro system to investigate the possibility of a cytogenetic adaptive response in female germ cells X-irradiated at 2 well characterized stages of maturation. Mouse ovarian follicles were cultured from an early preantral stage (1-2 layers of follicle cells) up to ovulation. Hormonal stimulation of ovulation was realized by addition of hCG and EGF to the culture medium on day 12. The follicles were X-irradiated with either 2 or 4 Gy preceded or not by 50 mGy (“conditioning dose”, 5 h earlier), on days 0 or 12 of the culture. Ovulated oocytes were collected on day 13, fixed and analyzed for the presence of chromosome aberrations. The results suggested that pre-irradiation of mouse pre-ovulatory oocytes with a low conditioning dose of X-rays could confer them some protection against radiation-induction of chromosome damage by a subsequent challenge dose of a few Gy.
KW - adaptive response
KW - oocytes
KW - follicle culture
KW - ionizing radiation
KW - chromosome aberrations
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_92270
UR - http://knowledgecentre.sckcen.be/so2/bibref/5250
M3 - Article
VL - 22
SP - 179
EP - 186
JO - In Vivo
JF - In Vivo
SN - 0258-851X
IS - 2
ER -
ID: 209565