Temporal Gene Expression of the Cyanobacterium Arthrospira in Response to Gamma Rays

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Temporal Gene Expression of the Cyanobacterium Arthrospira in Response to Gamma Rays. / Badri, Hanène; Monsieurs, Pieter; Coninx, Ilse; Nauts, Robin; Wattiez, Ruddy; Leys, Natalie.

In: PLOS ONE, Vol. 10, No. 8, e0135565, 08.2015, p. 1-29.

Research output: Contribution to journalArticlepeer-review

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@article{05851d251f174a30ae93dd438392d896,
title = "Temporal Gene Expression of the Cyanobacterium Arthrospira in Response to Gamma Rays",
abstract = "The edible cyanobacterium Arthrospira sp. PCC 8005 is resistant to ionising radiation. The cellular mechanisms underlying this radiation resistance are, however, still largely unknown. Therefore, additional molecular analysis was performed to investigate how these cells can escape from, protect against, or repair the radiation damage. Arthrospira cells were shortly exposed to different doses of 60Co gamma rays and the dynamic response was investigated by monitoring its gene expression and cell physiology at different time points after irradiation. The results revealed a fast switch from an active growth state to a kind of 'survival modus' during which the cells put photosynthesis, carbon and nitrogen assimilation on hold and activate pathways for cellular protection, detoxification, and repair. The higher the radiation dose, the more pronounced this global emergency response is expressed. This study provides new insights into phasic response and the cellular pathways involved in the radiation resistance of microbial cells, in particularly for photosynthetic organisms as the cyanobacterium Arthrospira.",
keywords = "arthrospira, cyanobacteria, ionizing radiation, microarray, proteomics",
author = "Han{\`e}ne Badri and Pieter Monsieurs and Ilse Coninx and Robin Nauts and Ruddy Wattiez and Natalie Leys",
note = "Score = 10",
year = "2015",
month = aug,
doi = "10.1371/journal.pone.0135565",
language = "English",
volume = "10",
pages = "1--29",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "PLOS - Public Library of Science",
number = "8",

}

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TY - JOUR

T1 - Temporal Gene Expression of the Cyanobacterium Arthrospira in Response to Gamma Rays

AU - Badri, Hanène

AU - Monsieurs, Pieter

AU - Coninx, Ilse

AU - Nauts, Robin

AU - Wattiez, Ruddy

AU - Leys, Natalie

N1 - Score = 10

PY - 2015/8

Y1 - 2015/8

N2 - The edible cyanobacterium Arthrospira sp. PCC 8005 is resistant to ionising radiation. The cellular mechanisms underlying this radiation resistance are, however, still largely unknown. Therefore, additional molecular analysis was performed to investigate how these cells can escape from, protect against, or repair the radiation damage. Arthrospira cells were shortly exposed to different doses of 60Co gamma rays and the dynamic response was investigated by monitoring its gene expression and cell physiology at different time points after irradiation. The results revealed a fast switch from an active growth state to a kind of 'survival modus' during which the cells put photosynthesis, carbon and nitrogen assimilation on hold and activate pathways for cellular protection, detoxification, and repair. The higher the radiation dose, the more pronounced this global emergency response is expressed. This study provides new insights into phasic response and the cellular pathways involved in the radiation resistance of microbial cells, in particularly for photosynthetic organisms as the cyanobacterium Arthrospira.

AB - The edible cyanobacterium Arthrospira sp. PCC 8005 is resistant to ionising radiation. The cellular mechanisms underlying this radiation resistance are, however, still largely unknown. Therefore, additional molecular analysis was performed to investigate how these cells can escape from, protect against, or repair the radiation damage. Arthrospira cells were shortly exposed to different doses of 60Co gamma rays and the dynamic response was investigated by monitoring its gene expression and cell physiology at different time points after irradiation. The results revealed a fast switch from an active growth state to a kind of 'survival modus' during which the cells put photosynthesis, carbon and nitrogen assimilation on hold and activate pathways for cellular protection, detoxification, and repair. The higher the radiation dose, the more pronounced this global emergency response is expressed. This study provides new insights into phasic response and the cellular pathways involved in the radiation resistance of microbial cells, in particularly for photosynthetic organisms as the cyanobacterium Arthrospira.

KW - arthrospira

KW - cyanobacteria

KW - ionizing radiation

KW - microarray

KW - proteomics

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_140780

UR - http://knowledgecentre.sckcen.be/so2/bibref/13034

U2 - 10.1371/journal.pone.0135565

DO - 10.1371/journal.pone.0135565

M3 - Article

VL - 10

SP - 1

EP - 29

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 8

M1 - e0135565

ER -

ID: 155843