The antifungal plant defensin HsAFP1 from Heuchera sanguinea induces apoptosis in Candida albicans

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The antifungal plant defensin HsAFP1 from Heuchera sanguinea induces apoptosis in Candida albicans. / Aerts, An; Bammens, Leen; Govaert, Gilmer; Carmona-Gutierrez, Didac; Madeo, Frank; Cammue, Bruno P.A.; Thevissen, Karin; Baatout, Sarah (Peer reviewer).

In: Frontiers in Microbiology, Vol. 2, 03.2011, p. 1-9.

Research output: Contribution to journalArticlepeer-review

Harvard

Aerts, A, Bammens, L, Govaert, G, Carmona-Gutierrez, D, Madeo, F, Cammue, BPA, Thevissen, K & Baatout, S 2011, 'The antifungal plant defensin HsAFP1 from Heuchera sanguinea induces apoptosis in Candida albicans', Frontiers in Microbiology, vol. 2, pp. 1-9. https://doi.org/10.3389/fmicb.2011.00047

APA

Aerts, A., Bammens, L., Govaert, G., Carmona-Gutierrez, D., Madeo, F., Cammue, B. P. A., Thevissen, K., & Baatout, S. (2011). The antifungal plant defensin HsAFP1 from Heuchera sanguinea induces apoptosis in Candida albicans. Frontiers in Microbiology, 2, 1-9. https://doi.org/10.3389/fmicb.2011.00047

Vancouver

Aerts A, Bammens L, Govaert G, Carmona-Gutierrez D, Madeo F, Cammue BPA et al. The antifungal plant defensin HsAFP1 from Heuchera sanguinea induces apoptosis in Candida albicans. Frontiers in Microbiology. 2011 Mar;2:1-9. https://doi.org/10.3389/fmicb.2011.00047

Author

Aerts, An ; Bammens, Leen ; Govaert, Gilmer ; Carmona-Gutierrez, Didac ; Madeo, Frank ; Cammue, Bruno P.A. ; Thevissen, Karin ; Baatout, Sarah. / The antifungal plant defensin HsAFP1 from Heuchera sanguinea induces apoptosis in Candida albicans. In: Frontiers in Microbiology. 2011 ; Vol. 2. pp. 1-9.

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@article{cd7344508f0244a9bf9a17917d34fe2d,
title = "The antifungal plant defensin HsAFP1 from Heuchera sanguinea induces apoptosis in Candida albicans",
abstract = "Plant defensins are active against plant and human pathogenic fungi (such as Candida albicans) and baker{\textquoteright}s yeast. However, they are non-toxic to human cells, providing a possible source for treatment of fungal infections. In this study, we characterized the mode of action of the antifungal plant defensin HsAFP1 from coral bells by screening the Saccharomyces cerevisiae deletion mutant library for mutants with altered HsAFP1 sensitivity and verified the obtained genetic data by biochemical assays in S. cerevisiae and C. albicans. We identified 84 genes, which when deleted conferred at least fourfold hypersensitivity or resistance to HsAFP1. A considerable part of these genes were found to be implicated in mitochondrial functionality. In line, sodium azide, which blocks the respiratory electron transport chain, antagonized HsAFP1 antifungal activity, suggesting that a functional respiratory chain is indispensable for HsAFP1 antifungal action. Since mitochondria are the main source of cellular reactive oxygen species (ROS), we investigated the ROS-inducing nature of HsAFP1. We showed that HsAFP1 treatment of C. albicans resulted in ROS accumulation. As ROS accumulation is one of the phenotypic markers of apoptosis in yeast, we could further demonstrate that HsAFP1 induced apoptosis in C. albicans.",
keywords = "plant defensin, Candida albicans, Saccharomyces cerevisiae, mitochondria, apoptosis, mode of action",
author = "An Aerts and Leen Bammens and Gilmer Govaert and Didac Carmona-Gutierrez and Frank Madeo and Cammue, {Bruno P.A.} and Karin Thevissen and Sarah Baatout",
note = "Score = 0",
year = "2011",
month = mar,
doi = "10.3389/fmicb.2011.00047",
language = "English",
volume = "2",
pages = "1--9",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers",

}

RIS - Download

TY - JOUR

T1 - The antifungal plant defensin HsAFP1 from Heuchera sanguinea induces apoptosis in Candida albicans

AU - Aerts, An

AU - Bammens, Leen

AU - Govaert, Gilmer

AU - Carmona-Gutierrez, Didac

AU - Madeo, Frank

AU - Cammue, Bruno P.A.

AU - Thevissen, Karin

A2 - Baatout, Sarah

N1 - Score = 0

PY - 2011/3

Y1 - 2011/3

N2 - Plant defensins are active against plant and human pathogenic fungi (such as Candida albicans) and baker’s yeast. However, they are non-toxic to human cells, providing a possible source for treatment of fungal infections. In this study, we characterized the mode of action of the antifungal plant defensin HsAFP1 from coral bells by screening the Saccharomyces cerevisiae deletion mutant library for mutants with altered HsAFP1 sensitivity and verified the obtained genetic data by biochemical assays in S. cerevisiae and C. albicans. We identified 84 genes, which when deleted conferred at least fourfold hypersensitivity or resistance to HsAFP1. A considerable part of these genes were found to be implicated in mitochondrial functionality. In line, sodium azide, which blocks the respiratory electron transport chain, antagonized HsAFP1 antifungal activity, suggesting that a functional respiratory chain is indispensable for HsAFP1 antifungal action. Since mitochondria are the main source of cellular reactive oxygen species (ROS), we investigated the ROS-inducing nature of HsAFP1. We showed that HsAFP1 treatment of C. albicans resulted in ROS accumulation. As ROS accumulation is one of the phenotypic markers of apoptosis in yeast, we could further demonstrate that HsAFP1 induced apoptosis in C. albicans.

AB - Plant defensins are active against plant and human pathogenic fungi (such as Candida albicans) and baker’s yeast. However, they are non-toxic to human cells, providing a possible source for treatment of fungal infections. In this study, we characterized the mode of action of the antifungal plant defensin HsAFP1 from coral bells by screening the Saccharomyces cerevisiae deletion mutant library for mutants with altered HsAFP1 sensitivity and verified the obtained genetic data by biochemical assays in S. cerevisiae and C. albicans. We identified 84 genes, which when deleted conferred at least fourfold hypersensitivity or resistance to HsAFP1. A considerable part of these genes were found to be implicated in mitochondrial functionality. In line, sodium azide, which blocks the respiratory electron transport chain, antagonized HsAFP1 antifungal activity, suggesting that a functional respiratory chain is indispensable for HsAFP1 antifungal action. Since mitochondria are the main source of cellular reactive oxygen species (ROS), we investigated the ROS-inducing nature of HsAFP1. We showed that HsAFP1 treatment of C. albicans resulted in ROS accumulation. As ROS accumulation is one of the phenotypic markers of apoptosis in yeast, we could further demonstrate that HsAFP1 induced apoptosis in C. albicans.

KW - plant defensin

KW - Candida albicans

KW - Saccharomyces cerevisiae

KW - mitochondria

KW - apoptosis

KW - mode of action

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_113385

UR - http://knowledgecentre.sckcen.be/so2/bibref/7992

U2 - 10.3389/fmicb.2011.00047

DO - 10.3389/fmicb.2011.00047

M3 - Article

VL - 2

SP - 1

EP - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

ER -

ID: 384116