The deletion of TonB-dependent receptor genes is part of the genome reduction process that occurs during adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung

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The deletion of TonB-dependent receptor genes is part of the genome reduction process that occurs during adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung. / Dingemans, Jozef; Ye, Lumeng; Hildebrand, Falk; Tontodonati, Francesca; Craggs, Michael; Bilocq, Florence; De Vos, Daniel; Crabbé, Aurélie; Van Houdt, Rob; Malfroot, Anne; Cornelis, Pierre.

In: Pathogens and Disease, Vol. 71, No. 3-4, 04.2014, p. 26-38.

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Harvard

Dingemans, J, Ye, L, Hildebrand, F, Tontodonati, F, Craggs, M, Bilocq, F, De Vos, D, Crabbé, A, Van Houdt, R, Malfroot, A & Cornelis, P 2014, 'The deletion of TonB-dependent receptor genes is part of the genome reduction process that occurs during adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung', Pathogens and Disease, vol. 71, no. 3-4, pp. 26-38. https://doi.org/10.1016/j.vetmic.2013.08.020

APA

Dingemans, J., Ye, L., Hildebrand, F., Tontodonati, F., Craggs, M., Bilocq, F., De Vos, D., Crabbé, A., Van Houdt, R., Malfroot, A., & Cornelis, P. (2014). The deletion of TonB-dependent receptor genes is part of the genome reduction process that occurs during adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung. Pathogens and Disease, 71(3-4), 26-38. https://doi.org/10.1016/j.vetmic.2013.08.020

Author

Dingemans, Jozef ; Ye, Lumeng ; Hildebrand, Falk ; Tontodonati, Francesca ; Craggs, Michael ; Bilocq, Florence ; De Vos, Daniel ; Crabbé, Aurélie ; Van Houdt, Rob ; Malfroot, Anne ; Cornelis, Pierre. / The deletion of TonB-dependent receptor genes is part of the genome reduction process that occurs during adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung. In: Pathogens and Disease. 2014 ; Vol. 71, No. 3-4. pp. 26-38.

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@article{23e8e2c866174337ac10e6a05293ab97,
title = "The deletion of TonB-dependent receptor genes is part of the genome reduction process that occurs during adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung",
abstract = "Chronic Pseudomonas aeruginosa infections are the main cause of morbidity among patients with cystic fibrosis (CF) due to persistent lung inflammation caused by interaction between this bacterium and the immune system. Longitudinal studies of clonally related isolates of a dominant CF clone have indicated that genome reduction frequently occurs during adaptation of P. aeruginosa in the CF lung. In this study, we have evaluated the P. aeruginosa population structure of patients attending the Universitair Ziekenhuis Brussel (UZ Brussel) CF reference center using a combination of genotyping methods. Although the UZ Brussel P. aeruginosa CF population is characterized by the absence of a dominant CF clone, some potential interpatient transmissions could be detected. Interestingly, one of these clones showed deletion of the alternative type I ferripyoverdine receptor gene fpvB. Furthermore, we found that several other TonB-dependent receptors are deleted as well. The genome of one potentially transmissible CF clone was sequenced, revealing large deleted regions including all type III secretion system genes and several virulence genes. Remarkably, a large number of deleted genes are shared between the P. aeruginosa CF clone described in this study and isolates belonging to the dominant Copenhagen CF DK2 clone, suggesting parallel evolution.",
keywords = "Pseudomonas, cystic fibrosis, iron",
author = "Jozef Dingemans and Lumeng Ye and Falk Hildebrand and Francesca Tontodonati and Michael Craggs and Florence Bilocq and {De Vos}, Daniel and Aur{\'e}lie Crabb{\'e} and {Van Houdt}, Rob and Anne Malfroot and Pierre Cornelis",
note = "Score = 10",
year = "2014",
month = apr,
doi = "10.1016/j.vetmic.2013.08.020",
language = "English",
volume = "71",
pages = "26--38",
journal = "Pathogens and Disease",
issn = "0378-1135",
publisher = "Elsevier",
number = "3-4",

}

RIS - Download

TY - JOUR

T1 - The deletion of TonB-dependent receptor genes is part of the genome reduction process that occurs during adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung

AU - Dingemans, Jozef

AU - Ye, Lumeng

AU - Hildebrand, Falk

AU - Tontodonati, Francesca

AU - Craggs, Michael

AU - Bilocq, Florence

AU - De Vos, Daniel

AU - Crabbé, Aurélie

AU - Van Houdt, Rob

AU - Malfroot, Anne

AU - Cornelis, Pierre

N1 - Score = 10

PY - 2014/4

Y1 - 2014/4

N2 - Chronic Pseudomonas aeruginosa infections are the main cause of morbidity among patients with cystic fibrosis (CF) due to persistent lung inflammation caused by interaction between this bacterium and the immune system. Longitudinal studies of clonally related isolates of a dominant CF clone have indicated that genome reduction frequently occurs during adaptation of P. aeruginosa in the CF lung. In this study, we have evaluated the P. aeruginosa population structure of patients attending the Universitair Ziekenhuis Brussel (UZ Brussel) CF reference center using a combination of genotyping methods. Although the UZ Brussel P. aeruginosa CF population is characterized by the absence of a dominant CF clone, some potential interpatient transmissions could be detected. Interestingly, one of these clones showed deletion of the alternative type I ferripyoverdine receptor gene fpvB. Furthermore, we found that several other TonB-dependent receptors are deleted as well. The genome of one potentially transmissible CF clone was sequenced, revealing large deleted regions including all type III secretion system genes and several virulence genes. Remarkably, a large number of deleted genes are shared between the P. aeruginosa CF clone described in this study and isolates belonging to the dominant Copenhagen CF DK2 clone, suggesting parallel evolution.

AB - Chronic Pseudomonas aeruginosa infections are the main cause of morbidity among patients with cystic fibrosis (CF) due to persistent lung inflammation caused by interaction between this bacterium and the immune system. Longitudinal studies of clonally related isolates of a dominant CF clone have indicated that genome reduction frequently occurs during adaptation of P. aeruginosa in the CF lung. In this study, we have evaluated the P. aeruginosa population structure of patients attending the Universitair Ziekenhuis Brussel (UZ Brussel) CF reference center using a combination of genotyping methods. Although the UZ Brussel P. aeruginosa CF population is characterized by the absence of a dominant CF clone, some potential interpatient transmissions could be detected. Interestingly, one of these clones showed deletion of the alternative type I ferripyoverdine receptor gene fpvB. Furthermore, we found that several other TonB-dependent receptors are deleted as well. The genome of one potentially transmissible CF clone was sequenced, revealing large deleted regions including all type III secretion system genes and several virulence genes. Remarkably, a large number of deleted genes are shared between the P. aeruginosa CF clone described in this study and isolates belonging to the dominant Copenhagen CF DK2 clone, suggesting parallel evolution.

KW - Pseudomonas

KW - cystic fibrosis

KW - iron

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_135660

UR - http://knowledgecentre.sckcen.be/so2/bibref/11581

U2 - 10.1016/j.vetmic.2013.08.020

DO - 10.1016/j.vetmic.2013.08.020

M3 - Article

VL - 71

SP - 26

EP - 38

JO - Pathogens and Disease

JF - Pathogens and Disease

SN - 0378-1135

IS - 3-4

ER -

ID: 257886