The Role of Trp53 in the Transcriptional Response to Ionizing Radiation in the Developing Brain

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The Role of Trp53 in the Transcriptional Response to Ionizing Radiation in the Developing Brain. / Verheyde, Joris; de Saint-Georges, Louis; Leyns, Luc; Benotmane, Rafi; Mergeay, Max (Peer reviewer).

In: DNA Research, Vol. 13, No. 2, 21.03.2006, p. 65-75.

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@article{f9b43f65b6064694a8219619ec8da94d,
title = "The Role of Trp53 in the Transcriptional Response to Ionizing Radiation in the Developing Brain",
abstract = "Brain formation results from a series of well-timed consecutive waves of cellular proliferation, migration and differentiation. Acute irradiation during pregnancy selectively interferes with these events to result in malformations such as microcephaly, reduced cortical thickness and mental retardation. In the present study we performed a straight-through cDNA-microarray analysis of the developing mouse brain at embryonic day E13, 3 h after in utero exposure to 50 cGy X-radiation. This dataset was used as an indication of genes involved in different pathways that are activated upon early radiation exposure, and for further evaluation using quantitative PCR (qPCR). Microarray and qPCR data revealed that the main activated pathways in irradiated wild-type embryos are involved in the regulation of a p53-mediated pathway that may lead to cell cycle delay/arrest and increased levels of apoptosis. To define whether the transcriptional radiation response was solely p53 mediated, we analysed the expression of cell cycle regulating genes in a Trp53 null mutant. The modulated expression of cell cycle regulating genes such as cyclins and Cdk genes indicated the induction of a cell cycle arrest, without evidence for the onset of apoptosis. ABSTRACT TRUNCATED AT 200 WORDS.",
keywords = "cDNA microarray, ionizing radiation, brain development, Trp53, p53",
author = "Joris Verheyde and {de Saint-Georges}, Louis and Luc Leyns and Rafi Benotmane and Max Mergeay",
note = "Score = 10",
year = "2006",
month = "3",
day = "21",
doi = "10.1093/dnares/dsi028",
language = "English",
volume = "13",
pages = "65--75",
journal = "DNA Research",
issn = "1340-2838",
publisher = "Oxford University Press",
number = "2",

}

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TY - JOUR

T1 - The Role of Trp53 in the Transcriptional Response to Ionizing Radiation in the Developing Brain

AU - Verheyde, Joris

AU - de Saint-Georges, Louis

AU - Leyns, Luc

AU - Benotmane, Rafi

A2 - Mergeay, Max

N1 - Score = 10

PY - 2006/3/21

Y1 - 2006/3/21

N2 - Brain formation results from a series of well-timed consecutive waves of cellular proliferation, migration and differentiation. Acute irradiation during pregnancy selectively interferes with these events to result in malformations such as microcephaly, reduced cortical thickness and mental retardation. In the present study we performed a straight-through cDNA-microarray analysis of the developing mouse brain at embryonic day E13, 3 h after in utero exposure to 50 cGy X-radiation. This dataset was used as an indication of genes involved in different pathways that are activated upon early radiation exposure, and for further evaluation using quantitative PCR (qPCR). Microarray and qPCR data revealed that the main activated pathways in irradiated wild-type embryos are involved in the regulation of a p53-mediated pathway that may lead to cell cycle delay/arrest and increased levels of apoptosis. To define whether the transcriptional radiation response was solely p53 mediated, we analysed the expression of cell cycle regulating genes in a Trp53 null mutant. The modulated expression of cell cycle regulating genes such as cyclins and Cdk genes indicated the induction of a cell cycle arrest, without evidence for the onset of apoptosis. ABSTRACT TRUNCATED AT 200 WORDS.

AB - Brain formation results from a series of well-timed consecutive waves of cellular proliferation, migration and differentiation. Acute irradiation during pregnancy selectively interferes with these events to result in malformations such as microcephaly, reduced cortical thickness and mental retardation. In the present study we performed a straight-through cDNA-microarray analysis of the developing mouse brain at embryonic day E13, 3 h after in utero exposure to 50 cGy X-radiation. This dataset was used as an indication of genes involved in different pathways that are activated upon early radiation exposure, and for further evaluation using quantitative PCR (qPCR). Microarray and qPCR data revealed that the main activated pathways in irradiated wild-type embryos are involved in the regulation of a p53-mediated pathway that may lead to cell cycle delay/arrest and increased levels of apoptosis. To define whether the transcriptional radiation response was solely p53 mediated, we analysed the expression of cell cycle regulating genes in a Trp53 null mutant. The modulated expression of cell cycle regulating genes such as cyclins and Cdk genes indicated the induction of a cell cycle arrest, without evidence for the onset of apoptosis. ABSTRACT TRUNCATED AT 200 WORDS.

KW - cDNA microarray

KW - ionizing radiation

KW - brain development

KW - Trp53

KW - p53

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_30511

UR - http://knowledgecentre.sckcen.be/so2/bibref/3521

U2 - 10.1093/dnares/dsi028

DO - 10.1093/dnares/dsi028

M3 - Article

VL - 13

SP - 65

EP - 75

JO - DNA Research

JF - DNA Research

SN - 1340-2838

IS - 2

ER -

ID: 315156