THERMOCOAX rhodium SPND sensitivity dispersion and validation of thef the sensitivity calculation model

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THERMOCOAX rhodium SPND sensitivity dispersion and validation of thef the sensitivity calculation model. / Vermeeren, Ludo; Leysen, Willem; Pichon, Laurent; Salou, Vincent; Helleux, Georges.

In: EPJ Web of Conferences, Vol. 225, 04015, 20.01.2020, p. 1-5.

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Vermeeren, Ludo ; Leysen, Willem ; Pichon, Laurent ; Salou, Vincent ; Helleux, Georges. / THERMOCOAX rhodium SPND sensitivity dispersion and validation of thef the sensitivity calculation model. In: EPJ Web of Conferences. 2020 ; Vol. 225. pp. 1-5.

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@article{12f5f77592824d12afb304437aa87db7,
title = "THERMOCOAX rhodium SPND sensitivity dispersion and validation of thef the sensitivity calculation model",
abstract = "Metallic copper to combat bacterial proliferation in drinking water systems is being investigated as an attractive alternative to existing strategies. A potential obstacle to this approach is the induction of metal resistance mechanisms in contaminating bacteria, that could severely impact inactivation efficacy. Thus far, the role of these resistance mechanisms has not been studied in conditions relevant to drinking water systems. Therefore, we evaluated the inactivation kinetics of Cupriavidus metallidurans CH34 in contact with metallic copper in drinking water. Viability and membrane permeability were examined for 9 days through viable counts and flow cytometry. After an initial drop in viable count, a significant recovery was observed starting after 48 h. This behavior could be explained by either a recovery from an injured/viable-but-non-culturable state or regrowth of surviving cells metabolizing lysed cells. Either hypothesis would necessitate an induction of copper resistance mechanisms, since no recovery was seen in a CH34 mutant strain lacking metal resistance mechanisms, while being more pronounced when copper resistance mechanisms were pre-induced. Interestingly, no biofilms were formed on the copper surface, while extensive biofilm formation was observed on the stainless steel control plates. When CH34 cells in water were supplied with CuSO4, a similar initial decrease in viable counts was observed, but cells recovered fully after 7 days. In conclusion, we have shown that long-term bacterial survival in the presence of a copper surface is possible upon the induction of metal resistance mechanisms. This observation may have important consequences in the context of the increasing use of copper as an antimicrobial surface, especially in light of potential co-selection for metal and antimicrobial resistance.",
keywords = "Nuclear measurements, Neutron detectors, Pulse shape discrimination, Reactor instrumentation, Modelling",
author = "Ludo Vermeeren and Willem Leysen and Laurent Pichon and Vincent Salou and Georges Helleux",
note = "Score=10",
year = "2020",
month = "1",
day = "20",
doi = "10.1051/epjconf/202022504015",
language = "English",
volume = "225",
pages = "1--5",
journal = "EPJ Web of Conferences",
issn = "2100-014X",
publisher = "EDP Sciences",

}

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TY - JOUR

T1 - THERMOCOAX rhodium SPND sensitivity dispersion and validation of thef the sensitivity calculation model

AU - Vermeeren, Ludo

AU - Leysen, Willem

AU - Pichon, Laurent

AU - Salou, Vincent

AU - Helleux, Georges

N1 - Score=10

PY - 2020/1/20

Y1 - 2020/1/20

N2 - Metallic copper to combat bacterial proliferation in drinking water systems is being investigated as an attractive alternative to existing strategies. A potential obstacle to this approach is the induction of metal resistance mechanisms in contaminating bacteria, that could severely impact inactivation efficacy. Thus far, the role of these resistance mechanisms has not been studied in conditions relevant to drinking water systems. Therefore, we evaluated the inactivation kinetics of Cupriavidus metallidurans CH34 in contact with metallic copper in drinking water. Viability and membrane permeability were examined for 9 days through viable counts and flow cytometry. After an initial drop in viable count, a significant recovery was observed starting after 48 h. This behavior could be explained by either a recovery from an injured/viable-but-non-culturable state or regrowth of surviving cells metabolizing lysed cells. Either hypothesis would necessitate an induction of copper resistance mechanisms, since no recovery was seen in a CH34 mutant strain lacking metal resistance mechanisms, while being more pronounced when copper resistance mechanisms were pre-induced. Interestingly, no biofilms were formed on the copper surface, while extensive biofilm formation was observed on the stainless steel control plates. When CH34 cells in water were supplied with CuSO4, a similar initial decrease in viable counts was observed, but cells recovered fully after 7 days. In conclusion, we have shown that long-term bacterial survival in the presence of a copper surface is possible upon the induction of metal resistance mechanisms. This observation may have important consequences in the context of the increasing use of copper as an antimicrobial surface, especially in light of potential co-selection for metal and antimicrobial resistance.

AB - Metallic copper to combat bacterial proliferation in drinking water systems is being investigated as an attractive alternative to existing strategies. A potential obstacle to this approach is the induction of metal resistance mechanisms in contaminating bacteria, that could severely impact inactivation efficacy. Thus far, the role of these resistance mechanisms has not been studied in conditions relevant to drinking water systems. Therefore, we evaluated the inactivation kinetics of Cupriavidus metallidurans CH34 in contact with metallic copper in drinking water. Viability and membrane permeability were examined for 9 days through viable counts and flow cytometry. After an initial drop in viable count, a significant recovery was observed starting after 48 h. This behavior could be explained by either a recovery from an injured/viable-but-non-culturable state or regrowth of surviving cells metabolizing lysed cells. Either hypothesis would necessitate an induction of copper resistance mechanisms, since no recovery was seen in a CH34 mutant strain lacking metal resistance mechanisms, while being more pronounced when copper resistance mechanisms were pre-induced. Interestingly, no biofilms were formed on the copper surface, while extensive biofilm formation was observed on the stainless steel control plates. When CH34 cells in water were supplied with CuSO4, a similar initial decrease in viable counts was observed, but cells recovered fully after 7 days. In conclusion, we have shown that long-term bacterial survival in the presence of a copper surface is possible upon the induction of metal resistance mechanisms. This observation may have important consequences in the context of the increasing use of copper as an antimicrobial surface, especially in light of potential co-selection for metal and antimicrobial resistance.

KW - Nuclear measurements

KW - Neutron detectors

KW - Pulse shape discrimination

KW - Reactor instrumentation

KW - Modelling

UR - https://ecm.sckcen.be/OTCS/llisapi.dll/open/39033649

U2 - 10.1051/epjconf/202022504015

DO - 10.1051/epjconf/202022504015

M3 - Article

VL - 225

SP - 1

EP - 5

JO - EPJ Web of Conferences

JF - EPJ Web of Conferences

SN - 2100-014X

M1 - 04015

ER -

ID: 6834158