Transcriptional response to ionizing radiation in lymphocyte subsets

Research output: Contribution to journalArticle

Authors

  • Rafi Benotmane
  • Marcella Mori
  • Tirone Ilaria
  • Elizabeth Hooghe-Peters
  • Christian Desaintes

Documents & links

Abstract

Human lymphocyte subpopulations differ in their cellular responses to ionizing radiation. To shed light on the molecular basis of this effect, we characterized the transcriptional response to 1 Gy X-rays of CD4+ T lymphocytes. Of 18,433 genes tested, 102 were modulated more than 1.5-fold. The majority of the strongly activated genes were p53 targets involved in DNA repair and apoptosis. The expression of three of these genes was further tested by quantitative RT-PCR in lymphocyte subpopulations [CD4+ and CD8+ T, CD19+ B, CD56+ natural killer cells and peripheral blood lymphocytes (PBLs)] from ten adult donors. In contrast to DDB2, TNFRSF10B and BAX were differentially modulated among the subpopulations and the PBLs, being more activated in irradiated CD19+ B and CD8+ T lymphocytes. The level of BAX activation in the various subpopulations correlated with the sensitivity of the cells to radiation, suggesting its possible role in the differential radiosensitivity of hematopoietic cell subsets.

Details

Original languageEnglish
Pages (from-to)1489-501
JournalCellular and Molecular Life Science
Volume62(13)
Issue number2005 Jul
Publication statusPublished - Jul 2005

Keywords

  • Lymphocytes, microarray, p53

ID: 275762