Abstract
A combined transcriptome and proteome analysis of mouse radiation-induced
AMLs using two primary AMLs, cell lines from these primaries, another cell line and
its in vivo passage is reported. Compared to haematopoietic progenitor and stem
cells (HPSC), over 5000 transcriptome alterations were identified, 2600 present in all
materials. 55 and 3 alterations were detected in the proteomes of the cell lines and
primary/in vivo passage material respectively, with one common to all materials. In
cell lines, approximately 50% of the transcriptome changes are related to adaptation
to cell culture, and in the proteome this proportion was higher. An AML ‘signature’ of
17 genes/proteins commonly deregulated in primary AMLs and cell lines compared to
HPSCs was identified and validated using human AML transcriptome data. This also
distinguishes primary AMLs from cell lines and includes proteins such as Coronin 1,
pontin/RUVBL1 and Myeloperoxidase commonly implicated in human AML. C-Myc was
identified as having a key role in radiation leukaemogenesis. These data identify novel
candidates relevant to mouse radiation AML pathogenesis, and confirm that pathways
of leukaemogenesis in the mouse and human share substantial commonality.
Details
Original language | English |
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Pages (from-to) | 40461-40480 |
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Journal | Oncotarget - Impact Journals |
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Volume | 7 |
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Issue number | 26 |
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DOIs | |
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Publication status | Published - 26 May 2016 |
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